Occurrence of&lt;i&gt; bla&lt;/i&gt;&lt;sub&gt;NDM-1&lt;/sub&gt; in a Clinical Isolate of &lt;i&gt;Acinetobacter lwoffii&lt;/i&gt; in Japan: Comparison of&lt;i&gt; bla&lt;/i&gt;&lt;sub&gt;NDM-1&lt;/sub&gt;-Harboring Plasmids between &lt;i&gt;A. lwoffii&lt;/i&gt; and &lt;i&gt;A. pittii&lt;/i&gt; Originated from a Hospital Sink

Hayashi, Wataru; Iimura, Masaki; Horiuchi, Kazuki; Arai, Eriko; Natori, Tatsuya; Suzuki, Shin; Matsumoto, Go; Izumi, Katsutoshi; Yoshida, Satoshi; Nagano, Yukiko; Nagano, Noriyuki

doi:10.7883/yoken.jjid.2020.806

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…A recent review listed 33 rep groups (GRs) (Salgado-Camargo et al ., 2020), and we have since described an additional group, GR34 (Liu et al ., 2021). Plasmids carrying clinically-significant antibiotic resistance genes (ARGs) have been reported in A. baumannii (Blackwell and Hall, 2017; Hamidian et al ., 2016; Hamidian and Hall, 2014; Liu et al ., 2015; Nigro and Hall, 2014) and in other Acinetobacter species (Alattraqchi et al ., 2021; Hayashi et al ., 2021; Li et al ., 2021; Silva et al ., 2018; Yang et al ., 2021), clearly indicating their important role in the emergence and transmission of antimicrobial resistance in this genus. Few plasmid groups have been the subject of comparative analyses, so how the remaining types evolve or are distributed, geographically and throughout the Acinetobacter genus, is poorly understood and their genetic structures remain largely undescribed.…”

Section: Introductionmentioning

confidence: 99%

“…baumannii (Blackwell and Hall, 2017;Hamidian et al, 2016;Hamidian and Hall, 2014;Liu et al, 2015; and in other Acinetobacter species (Alattraqchi et al, 2021;Hayashi et al, 2021;Li et al, 2021;Silva et al, 2018;Yang et al, 2021), clearly indicating their important role in the emergence and transmission of antimicrobial resistance in this genus. Few plasmid groups have been the subject of comparative analyses, so how the remaining types evolve or are distributed, geographically and throughout the Acinetobacter genus, is poorly understood and their genetic structures remain largely undescribed.…”

Section: Introductionmentioning

confidence: 99%

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GR13-type plasmids in Acinetobacter potentiate the accumulation and horizontal transfer of diverse accessory genes

Moran

Liu

Doughty

et al. 2022

Preprint

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Carbapenem resistance and other antibiotic resistance genes (ARGs) can be found in plasmids in Acinetobacter, but many plasmid types in this genus have not been well-characterised. Here we describe the distribution, diversity and evolutionary capacity of rep group 13 (GR13) plasmids that are found in Acinetobacter species from diverse environments. Our investigation was prompted by the discovery of two GR13 plasmids in A. baumannii isolated in an intensive care unit (ICU). The plasmids harbour distinct accessory genes: pDETAB5 contains blaNDM-1 and genes that confer resistance to four further antibiotic classes, while pDETAB13 carries putative alcohol tolerance determinants. Both plasmids contain multiple dif modules, which are flanked by pdif sites recognised by XerC/XerD tyrosine recombinases. The ARG-containing dif modules in pDETAB5 are almost identical to those found in pDETAB2, a GR34 plasmid from an unrelated A. baumannii isolated in the same ICU a month prior. Examination of a further 41 complete, publicly available plasmid sequences revealed that the GR13 pangenome consists of just four core but 1086 accessory genes, 123 in the shell and 1063 in the cloud, reflecting substantial capacity for diversification. The GR13 core genome includes genes for replication and partitioning, and for a putative tyrosine recombinase. Accessory segments encode proteins with diverse putative functions, including for metabolism, antibiotic/heavy metal/alcohol tolerance, restriction-modification, an anti-phage system and multiple toxin-antitoxin systems. The movement of dif modules and actions of insertion sequences play an important role in generating diversity in GR13 plasmids. Discrete GR13 plasmid lineages are internationally disseminated and found in multiple Acinetobacter species, which suggests they are important platforms for the accumulation, horizontal transmission and persistence of accessory genes in this genus.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GR13-type plasmids in Acinetobacter potentiate the accumulation and horizontal transfer of diverse accessory genes

Moran

Liu

Doughty

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…A recent review listed 33 rep groups [10], and we have since described an additional group, GR34 [11]. Plasmids carrying clinically significant antibiotic resistance genes (ARGs) have been reported in A. baumannii [12][13][14][15][16] and in other Acinetobacter species [7,8,[17][18][19], clearly indicating their important role in the emergence and transmission of antimicrobial resistance in this genus. Few plasmid groups have been the subject of comparative analyses, so how the remaining types evolve or are distributed, geographically and throughout the Acinetobacter genus, is poorly understood and their genetic structures remain largely undescribed.…”

Section: Introductionmentioning

confidence: 99%

GR13-type plasmids in Acinetobacter potentiate the accumulation and horizontal transfer of diverse accessory genes

et al. 2022

View full text Add to dashboard Cite

Carbapenem and other antibiotic resistance genes (ARGs) can be found in plasmids in Acinetobacter , but many plasmid types in this genus have not been well-characterized. Here we describe the distribution, diversity and evolutionary capacity of rep group 13 (GR13) plasmids that are found in Acinetobacter species from diverse environments. Our investigation was prompted by the discovery of two GR13 plasmids in A. baumannii isolated in an intensive care unit (ICU). The plasmids harbour distinct accessory genes: pDETAB5 contains bla NDM-1 and genes that confer resistance to four further antibiotic classes, while pDETAB13 carries putative alcohol tolerance determinants. Both plasmids contain multiple dif modules, which are flanked by pdif sites recognized by XerC/XerD tyrosine recombinases. The ARG-containing dif modules in pDETAB5 are almost identical to those found in pDETAB2, a GR34 plasmid from an unrelated A. baumannii isolated in the same ICU a month prior. Examination of a further 41 complete, publicly available plasmid sequences revealed that the GR13 pangenome consists of just four core but 1186 accessory genes, 123 in the shell and 1063 in the cloud, reflecting substantial capacity for diversification. The GR13 core genome includes genes for replication and partitioning, and for a putative tyrosine recombinase. Accessory segments encode proteins with diverse putative functions, including for metabolism, antibiotic/heavy metal/alcohol tolerance, restriction-modification, an anti-phage system and multiple toxin–antitoxin systems. The movement of dif modules and actions of insertion sequences play an important role in generating diversity in GR13 plasmids. Discrete GR13 plasmid lineages are internationally disseminated and found in multiple Acinetobacter species, which suggests they are important platforms for the accumulation, horizontal transmission and persistence of accessory genes in this genus.

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“…14,16 In Africa, several investigators have reported the occurrence of NDM-1 amongst members of enteric bacteria, including Klebsiella species, Enterobacter cloacae, Citrobacter freundii, Serratia marcescens, Acinetobacter baumannii etc. [17][18][19][20][21] in South Africa, Kenya, Ethiopia and other eastern/southern African countries. In Nigeria, NDM-1 has been reported amongst Gram-negative enteric bacteria in various geopolitical zone, including the Southwestern region (Lagos), Northeastern region (Maiduguri), South-southern region (Bayelsa), North-western region (Kano), and South-eastern region (Enugu) of the country [22][23][24][25][26][27][28][29] however, non has reported NDM-1 amongst Gram-positive strains.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of New Delhi Metallo-Beta-Lactamase 1 ProducingEnterococcusSpecies in Oghara Water Nexus: An Emerging Environmental Implications of Resistance Dynamics

Esegbuyota

Onohuean

Oyama³

2022

Microbiol�Insights

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Various members of the enteric bacteria in recent times are evolving diverse survival mechanisms for antibiotic therapy resulting in failure of treatment in infection and disease cases. The Enterococcus species are potential strains implicated in gastrointestinal tract infection and are recently evolving in the resistance mechanism. The study evaluates the occurrence of New Delhi Metallo-beta-lactamase 1 (NDM-1) amongst Enterococcus species using the phenotypic and genomic characterization of environmental strains in the Oghara water nexus. Presumptive isolates of Enterococcus species were retrieved from various sampled water sources and confirmed using polymerase chain reaction (PCR). Antibiotic susceptibility testing was conducted on confirmed isolates using Kirby-Bauer disk diffusion methods. The result reveals 63 genus isolates confirmed Enterococcus species, of which 42 (67%) were Enterococcus faecium, 15 (23%) were Enterococcus faecalis, and 6 (10%) were other Enterococcus species. Fourteen among the E. faecalis isolates show resistance to Ertapenem-EDTA, while 17 (44.8%) of the E. faecium show resistance to Ertapenem-EDTA to presumptively reveal their NDM-1 phenotype. The PCR detection of the NDM-1 gene further confirmed 23 (36.5%) isolates as positive genotypes amongst the isolates that previously showed presumptive NDM-1 phenotype. It was also observed that 10 (15.9%) of Enterococcus faecium members harbored the NDM-1 genotype, whereas 8 (12.7%) members of the Enterococcus faecalis harbored the NDM-1 genotype. The observation of such resistance determinants necessitates a call for the adroit application of relevant therapeutics in the management of related infections and an environmental health caution to prevent the spread of such resistance potential enteric bacteria pathogens.

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Occurrence of<i> bla</i><sub>NDM-1</sub> in a Clinical Isolate of <i>Acinetobacter lwoffii</i> in Japan: Comparison of<i> bla</i><sub>NDM-1</sub>-Harboring Plasmids between <i>A. lwoffii</i> and <i>A. pittii</i> Originated from a Hospital Sink

Abstract: Occurrence of blaNDM-1 in a clinical isolate of Acinetobacter lwoffii in Japan: comparison of blaNDM-1-harboring plasmids between A. lwoffii and A. pittii originated from the hospital sink

Cited by 4 publications

References 13 publications

GR13-type plasmids in Acinetobacter potentiate the accumulation and horizontal transfer of diverse accessory genes

GR13-type plasmids in Acinetobacter potentiate the accumulation and horizontal transfer of diverse accessory genes

GR13-type plasmids in Acinetobacter potentiate the accumulation and horizontal transfer of diverse accessory genes

Occurrence of New Delhi Metallo-Beta-Lactamase 1 ProducingEnterococcusSpecies in Oghara Water Nexus: An Emerging Environmental Implications of Resistance Dynamics

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