Occurrence of Pseudomonas syringae pv. coryli on Hazelnut Orchards in Sicily, Italy and Characterization by Fluorescent Amplified Fragment Length Polymorphism

Abstract

Field surveys for ascertaining the sanitary status of hazelnut (Corylus avellana L.) orchards located in the provinces of Catania and Messina, Sicily, were carried out. Twig and branch diebacks were frequently observed on the local hazelnut cultivars. Identification of the bacterial pathogen associated with these symptoms was performed by means of biochemical, pathogenicity and molecular tests as well as by using fluorescent amplified fragment length polymorphic (fAFLP) analysis. The study revealed, for the first time, the presence of Pseudomonas syringae pv. coryli in the Sicilian hazelnut orchards. All the strains obtained did not show the presence of syrB gene. The fAFLP analysis pointed out the presence of three different lineages within the strains of this pathogen.

A pith necrosis caused by Xanthomonas perforans on tomato plants

“…fAFLP analysis was carried out as previously reported by Vos et al (1995) and modified by Cirvilleri et al (2007). DNA was digested with FastDigest EcoRI and MseI (Fermentas, Thermo Fisher Scientific Inc., Waltham, MA, USA) and EcoRI and MseI adaptors ligated C using two units of T4 DNA ligase.…”

Disease and Frost Damage of Woody Plants Caused by Pseudomonas syringae

“…Firstly, in planta pathogenicity tests on the original host of isolation, for the completion of Koch's postulate, is lacking in some cases (Gilbert et al, 2009;Ivanovic et al, 2012;Kaluzna et al, 2010). Secondly, host range tests have been performed only in a limited number of studies and on a very low number of host species (1-5) (Cirvilleri et al, 2007;Eltlbany et al, 2012;Golzar and Cother, 2008;Hall et al, 2004;Psallidas, 1993;Samavatian, 2006;Scortichini, 2006;Scortichini et al, 2005;Taghavi and Hasani, 2012;Vicente et al, 2004) making it difficult to know if the described pathovar is really different from previously described pathovars. On Prunus spp., where different pathovars cause the disease, cross-infection tests within the genus are lacking.…”