Occurrence of
            <i>mcr-1</i>
            in Colistin-Resistant Salmonella enterica Isolates Recovered from Humans and Animals in Italy, 2012 to 2015

Carnevali, Chiara; Morganti, Marina; Scaltriti, Erika; Bolzoni, Luca; Pongolini, Stefano; Casadei, Gabriele

doi:10.1128/aac.01803-16

Cited by 58 publications

(65 citation statements)

References 6 publications

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“…These resistant bacteria might contaminate meat during slaughtering procedures and transfer to humans by food chain or to workers via direct animal contact as indicated in some previous studies (Angulo et al, 2004; Liebana et al, 2013). Recently, several studies suggested the possibility of the transference MCR-1-producing Enterobacteriaceae to humans via food chain (Campos et al, 2016; Carnevali et al, 2016; Figueiredo et al, 2016; Wang et al, 2017b). Hence it is urgent to limit the usage of polymyxins (colistin) in veterinary medicine especially as feed additives in China.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Colistin Resistance and mcr-1 Gene in Escherichia coli Isolated from Food Animals in China

Huang

Chen

et al. 2017

Front. Microbiol.

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The objective of this study was to determine the minimal inhibitory concentration of colistin for Escherichia coli from food animals and the possible underlying colistin resistance mechanisms. During 2007–2014, 4,438 E. coli isolates of food animal origins were collected. The susceptibility of colistin was tested by the agar dilution method. Mutations in pmrA, pmrB, and mgrB and the presence of mcr-1 gene were determined by PCR and DNA sequencing. Complementation experiments were carried out to evaluate the contribution of the mutations to colistin resistance. There was a high frequency of colistin resistance in E. coli from pigs on farm (24.1%) and at slaughter (24.3%) in 2013–2014, followed by chickens on farm (14.0%) and at slaughter (9.5%). The resistance frequency of E. coli in cow isolates was the lowest (0.9%). MIC distribution for colistin showed that most isolates (75.2%) were distributed at 0.25 mg/L–0.5 mg/L, followed by 4 mg/L–8 mg/L (16.8%). Compared with the isolates from pigs and chickens recovered during 2013–2014, E. coli isolates collected during 2007–2008 (5.5%) and 2010–2011 (12.4%) showed significantly lower frequency of colistin resistance (P < 0.05). DNA sequencing and complementation experiments failed to detect any insertion inactivation or mutation in pmrA, pmrB, and mgrB associated with colistin resistance. However, 91.0% colistin-resistant isolates were positive for mcr-1. The high frequency of colistin resistance and mcr-1 gene among E. coli isolates from food animals in China urged the need to minimize potential risks of colistin resistance development and the spread of mcr-1 gene.

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Section: Discussionmentioning

confidence: 99%

High Prevalence of Colistin Resistance and mcr-1 Gene in Escherichia coli Isolated from Food Animals in China

Huang

Chen

et al. 2017

Front. Microbiol.

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“…In 2015, a colistin-resistant E s cherichia coli isolate from a pig farm in Shanghai was found to contain mcr1 — a plasmid-borne gene that encodes a phosphoethanolamine transferase 50 . This finding, and the subsequent discovery of mcr1 on plasmids worldwide 51–59 (see the figure), foreshadows the fall of colistin as a last-resort antibiotic that lacks transferable plasmid-encoded resistance. With the high levels of antibiotic prescription in medicine and unregulated antibiotic use in agriculture around the world it is likely that it is only a matter of time before completely pan-resistant plasmids are found that combine the above resistance mechanisms.…”

Section: Figurementioning

confidence: 96%

“…Agricultural resistomes are of particular interest because the selective pressures of heavy antibiotic use and extensive anthropogenic interaction make this a high-risk habitat for antibiotic resistance selection and dissemination. Indeed, the mcr1 plasmid-mediated colistin resistance determinant was first discovered on a Chinese pig farm 50 , although it has since been detected in new and banked clinical and environmental samples across the globe 51–59 (BOX 2). …”

Section: High-throughput Resistance Discoverymentioning

confidence: 99%

Next-generation approaches to understand and combat the antibiotic resistome

2017

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Antibiotic resistance is a natural feature of diverse microbial ecosystems. Although recent studies of the antibiotic resistome have highlighted barriers to the horizontal transfer of antibiotic resistance genes between habitats, the rapid global spread of genes that confer resistance to carbapenem, colistin and quinolone antibiotics illustrates the dire clinical and societal consequences of such events. Over time, the study of antibiotic resistance has grown from focusing on single pathogenic organisms in axenic culture to studying antibiotic resistance in pathogenic, commensal and environmental bacteria at the level of microbial communities. As the study of antibiotic resistance advances, it is important to incorporate this comprehensive approach to better inform global antibiotic resistance surveillance and antibiotic development. It is increasingly becoming apparent that although not all resistance genes are likely to geographically and phylogenetically disseminate, the threat presented by those that are is serious and warrants an interdisciplinary research focus. In this Review, we highlight seminal work in the resistome field, discuss recent advances in the studies of resistomes, and propose a resistome paradigm that can pave the way for the improved proactive identification and mitigation of emerging antibiotic resistance threats.

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“…best of our knowledge, this is the first detection of mcr-1 gene in S. Newport in humans in China, even though mcr-1 has been previously reported in S. Newport isolates from pigs and chicken in Italy [14,15], and China [16,17].…”

mentioning

confidence: 64%

Emerging colistin resistance in Salmonella enterica serovar Newport isolates from human infections

Elbediwi

Pan

Biswas

et al. 2020

Emerging Microbes & Infections

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Worldwide emergence of Salmonella enterica serovar Newport (S. Newport) infection in humans, in parallel with a significant increasing prevalence of antimicrobial resistance (AR), is a serious public health concern. However, the prevalence of S. Newport resistance in China remains largely unknown. A retrospective study of 287 S. Newport clinical isolates collected during 1997-2018 was undertaken for characterization of AR profiles using the micro-dilution assay. We found a recent emergence of colistin resistance in four Chinese clinical isolates, including mcr-1-positive isolates. Importantly, phylogenomic and microbiological investigations indicate multiple independent clonal transmission of colistin-resistant S. Newport isolates of different seafood origins. Our study highlights potential reservoirs for transmission of colistin resistance and suggests that the global food supply chain may facilitate this dissemination.

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Occurrence of mcr-1 in Colistin-Resistant Salmonella enterica Isolates Recovered from Humans and Animals in Italy, 2012 to 2015

Abstract: The first case of plasmid-mediated resistance to colistin due to the genemcr-1was reported in China in late 2015 (1).…

Cited by 58 publications

References 6 publications

High Prevalence of Colistin Resistance and mcr-1 Gene in Escherichia coli Isolated from Food Animals in China

High Prevalence of Colistin Resistance and mcr-1 Gene in Escherichia coli Isolated from Food Animals in China

Next-generation approaches to understand and combat the antibiotic resistome

Emerging colistin resistance in Salmonella enterica serovar Newport isolates from human infections

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