, et al.. One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions. Veterinary Microbiology, Elsevier, 2011, 149 (3-4) Please cite this article as: Martelli, P., Ferrari, L., Morganti, M., De Angelis, E., Bonilauri, P., Guazzetti, S., Caleffi, A., Borghetti, P., One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions, Veterinary Microbiology (2010Microbiology ( ), doi:10.1016Microbiology ( /j.vetmic.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 This study investigated the efficacy of a one-dose porcine circovirus 2 (PCV2) subunit 22 vaccine based on the PCV2 Cap protein expressed in a baculovirus system on two different farms at 23 which a history of porcine circovirus-associated disease (PCVD) was present. Morbidity, mortality, 24 average daily weight gain, carcass weight, PCV2 load in serum and vaccine immunogenicity were 25 assessed. Serology to porcine reproductive and respiratory syndrome virus (PRRSV) and 26Mycoplasma hyopneumoniae was performed. A double-blind, randomised, and controlled field trial 27 was performed distributing 818 piglets between two treatment groups. At inclusion (weaning at 21 28 ± 3 days of age), 408 animals (group B) received a 2-mL intramuscular dose of Porcilis PCV between 12 and 26 weeks of age when viremia and the specific disease occurred) as well as carcass 38 weight at slaughter age (+4.5 kg). These effects were associated with virologic and clinical 39 protection from the immunogenicity of the vaccine measured as activation of both a humoral and a 40 cellular immune response. 41 42

The first case of plasmid-mediated resistance to colistin due to the genemcr-1was reported in China in late 2015 (1).…

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The quantitative and qualitative patterns of environmental contamination by Listeria monocytogenes were investigated in the production chain of dry-cured Parma ham. Standard arrays of surfaces were sampled in processing facilities during a single visit per plant in the three compartments of the food chain, i.e., ham production (19 plants) and postproduction, which was divided into deboning (43 plants) and slicing (25 plants) steps. The numbers of sampled surfaces were 384 in ham production, with 25 positive for L. monocytogenes, and 1,084 in postproduction, with 83 positives. Statistical analysis of the prevalence of contaminated surfaces showed that in ham production, contamination was higher at the beginning of processing and declined significantly toward the end, while in postproduction, prevalence rose toward the end of processing. Prevalence was higher in the deboning facilities than in slicing facilities and was dependent on the type of surface (floor/drainage > clothing > equipment). The qualitative pattern of contamination was investigated through an analysis of the survey isolates and a set of isolates derived from routine monitoring, including longitudinal isolations. Pulsed-field gel electrophoresis (PFGE) and whole-genome singlenucleotide polymorphism (SNP) analysis revealed a remarkable clonality of L. monocytogenes within plants, with the detection of 16 plant-specific clones out of 17 establishments with multiple isolates. Repeated detections of clonal isolates >6 months apart were also observed. Six was the maximum number of between-isolate differences in core SNPs observed within these clones. Based on the same six-SNP threshold, three clusters of clonal isolates, shared by six establishments, were also identified. The spread of L. monocytogenes within and between plants, as indicated by its clonal behavior, is a matter of concern for the hygienic management of establishments.