Occurrence of human astrovirus associated with gastroenteritis among Congolese children in Brazzaville, Republic of Congo

Tsague, Boris Nguekeng; Louya, Vivaldie Mikounou; Ntoumi, Francine; Adedoja, Ayodele; Vouvoungui, Christevy; Peko, Simon Marie; Abena, Ange Antoine

doi:10.1016/j.ijid.2020.02.056

Cited by 12 publications

(9 citation statements)

References 23 publications

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“…In the current study, astrovirus was detected in 11% of children with acute gastroenteritis. This result matches recent reports in the Republic of Congo (10.3%) 13 and India (12.5%). 14 However, our finding is higher than previous results in Kenya (6.3%), 15 Northwest Ethiopia (3.6%), 8 lower than others obtained in Egypt by El Taweel et al (2020): 28%, 16 and Nigeria: 40.4%.…”

Section: Discussionsupporting

confidence: 92%

“…Astrovirus diarrhea is a common infection both in developed and developing countries, which suggests that the improvement of the hygiene and living conditions alone cannot prevent such infection. 8,[13][14][15][16][17][18] Development of an astrovirus vaccine and its administration in early life may have a role in reducing this infection in early life and even decrease the incidence of astrovirus diarrhea with increasing age. 19 Regarding the clinical features associated with diarrhea in HAstV positive patients, the degrees of dehydration (mild, moderate, and severe) were detected in astrovirus positive cases with no significant association of classic HAstV with any of them (p=0.463).…”

Section: Discussionmentioning

confidence: 99%

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Molecular study of human astrovirus in Egyptian children with acute gastroenteritis

Zaki¹,

Mashaly²,

Alsayed³

et al. 2020

Germs

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Introduction Human astrovirus (HAstV) has been increasingly identified as an important cause of acute gastroenteritis in young children. Limited information is available about the prevalence and genotype distribution of classic HAstV causing acute gastroenteritis in Egyptian children.Methods Stool samples were collected from 100 infants and children attending the gastroenterology outpatient clinic in Mansoura University Children Hospital and suffering from acute gastroenteritis during the period extending from January 2018 to January 2019. Samples were tested for HAstV using reverse transcription PCR. Genotyping was performed using type-specific reverse transcription nested PCR.Results Among 100 children included in this study, the detection rate of HAstV was 11% (11 patients). There was a significant difference regarding age between cases positive and negative for HAstV (p=0.005). There was a higher prevalence of HAstV in children aged one year or younger. Significant association was detected between HAstV positive cases and rural residence (p=0.002), summer season (p=0.025) and fever (p=0.017). The HAstV genotypes detected were HAstV-8 (8/11, 72.7%), HAstV-3 (2/11, 18.2%) and HAstV-2 (1/11, 9.1%).Conclusions This study suggests that HAstV is a common pathogen causing gastroenteritis in Egyptian children especially in rural areas. The most frequent HAstV genotype in our study was HAstV-8. Keywords Astrovirus, gastroenteritis, diarrhea, children, genotypes. Introduction1Acute gastroenteritis or infectious diarrhea is a common cause of morbidity and mortality in children below five years. The reported mortality rates in 2016 were 446,000 for children below 5 years. Most of these deaths are in developing

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Molecular study of human astrovirus in Egyptian children with acute gastroenteritis

Zaki¹,

Mashaly²,

Alsayed³

et al. 2020

Germs

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“…Among the 100 studied children with AGE, norovirus and astrovirus were detected in 27% and 12%, respectively. These prevalence rates were similar to previous studies from Egypt [ 32 ], the Republic of Congo (10.3%) [ 33 ], and India (12.5%) [ 34 ]. Nevertheless, the rates were higher than reported in a previous study from Kenya (6.3%) [ 35 ].…”

Section: Discussionsupporting

confidence: 91%

Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus

et al. 2022

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Background and aim Human parechovirus (HPeV) has emerged as a pathogen associated with acute gastroenteritis (AGE). Aim To detect the presence of HPeV in the stool samples from Egyptian children with AGE seeking care and the possibility of its co-infection with other enteric viruses. Methodology One hundred stool samples were collected from children attending Mansoura University Children's Hospital with AGE. HPeV and astrovirus were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). At the same time, detection of rotavirus antigen and norovirus was achieved by enzyme-linked immunosorbent assay and rapid immunochromatographic method, respectively. Results The most frequently detected virus was rotavirus (39%), followed by norovirus (27%), HPeV (19%), and astrovirus (12%). Interestingly, the single infection with HPeV was 5%. Among the 19 HPeV positive samples, the co-infection of HPeV with other enteric viruses was detected in 9(43.9%) for rotavirus, 7(36.8%) for norovirus, 2(10.5%) for astrovirus, in 3(15.8%) for rotavirus and norovirus and 1(5.3%) for norovirus and astrovirus. Regarding the clinical presentation, there was no significant difference between children infected with HPeV alone and those infected with viruses other than HPeV alone; fever (p = 0.3), vomiting (p = 0.12), abdominal pain (p = 0.12), and grades of severity (P = 0.82). HPeV alone infected children were of mild severity (60%), and their main presenting symptom was fever (60%). Conclusions Detection of HPeV as a single viral pathogen in the stool of some children with AGE showed that this virus could be a causative agent of AGE in Egyptian children. Therefore, HPeV could be included as one of the viruses screened for AGE diagnosis in children in Egypt.

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“…1,2 Among the astroviruses, human astrovirus (HAstV) was first identified in the mid-1970s, 3 and is a major cause of acute watery diarrhea and fever in vulnerable populations such as the elderly, children, and individuals with compromised immunity. 2,4 HAstV infections typically manifest as watery diarrhea, fever, and vomiting in some cases, 3 and have become a frequent and leading cause of public diarrheal illness in recent decades. 3 Therefore, understanding the genetic characteristics of HAstV is crucial for improving surveillance efforts.…”

Section: Introductionmentioning

confidence: 99%

“…Astroviruses are a family of enteric, nonenveloped, spherical, single‐stranded, positive‐sense RNA viruses that typically cause gastroenteritis in both mammals and birds 1,2 . Among the astroviruses, human astrovirus (HAstV) was first identified in the mid‐1970s, 3 and is a major cause of acute watery diarrhea and fever in vulnerable populations such as the elderly, children, and individuals with compromised immunity 2,4 . HAstV infections typically manifest as watery diarrhea, fever, and vomiting in some cases, 3 and have become a frequent and leading cause of public diarrheal illness in recent decades 3 .…”

Section: Introductionmentioning

confidence: 99%

Genetic characteristics of classical astroviruses in Shenzhen, China, 2016–2019

Hu,

Wang,

et al. 2023

Journal of Medical Virology

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Human astrovirus (HAstV) is a single‐stranded, positive‐sense RNA virus and is the leading cause of viral gastroenteritis. However, despite its prevalence, astroviruses still remain one of the least studied enteroviruses. In this study, we sequenced 11 classical astrovirus strains from clinical samples collected in Shenzhen, China from 2016 to 2019, analyzed their genetic characteristics, and deposited them into GenBank. We conducted phylogenetic analysis using IQ‐TREE software, with references to astrovirus sequences worldwide. The phylogeographic analysis was performed using the Bayesian Evolutionary Analysis Sampling Trees program, through Bayesian Markov Chain Monte Carlo sampling. We also conducted recombination analysis with the Recombination Detection Program. The newly sequenced strains were categorized as HAstV genotype 1, which is the predominant genotype in Shenzhen. Phylogeographic reconstruction indicated that HAstV‐1 may have migrated from the United States to China, followed by frequent transmission between China and Japan. The recombination analysis revealed recombination events within and across genotypes, and identified a recombination‐prone region that produced relatively uniform recombination breakpoints and fragment lengths. The genetic analysis of HAstV strains in Shenzhen addresses the current lack of astrovirus data in the region of Shenzhen and provides key insights to the evolution and transmission of astroviruses worldwide. These findings highlight the importance of improving surveillance of astroviruses.

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Occurrence of human astrovirus associated with gastroenteritis among Congolese children in Brazzaville, Republic of Congo

Cited by 12 publications

References 23 publications

Molecular study of human astrovirus in Egyptian children with acute gastroenteritis

Molecular study of human astrovirus in Egyptian children with acute gastroenteritis

Viral pathogens of acute gastroenteritis in Egyptian children: role of the parechovirus

Genetic characteristics of classical astroviruses in Shenzhen, China, 2016–2019

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