Occurrence of Fusarium species and mycotoxins in Swiss oats—Impact of cropping factors

Schöneberg, Torsten; Jenny, Eveline; Wettstein, Felix E.; Bucheli, Thomas D.; Mascher, Fabio; Bertossa, Mario; Musa, Tomke; Seifert, Keith A.; Gräfenhan, Tom; Keller, Beat; Vogelgsang, Susanne

doi:10.1016/j.eja.2017.09.004

Cited by 61 publications

(94 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA was extracted from 50 ± 2 mg oat flour, weighed into 1.2 mL polypropylene cluster tubes (Corning), using the NucleoSpin 96 Plant II kit (Macherey‐Nagel) with the aid of one autoclaved tungsten bead per tube, as described in Schöneberg et al . (). The DNA concentration was determined by measuring the emission of the fluorescent Hoechst 33258 (Fluorescent DNA Quantitation kit; Bio‐Rad) bound to double‐stranded DNA, based on the emitted fluorescence of a known concentration of serially diluted DNA standard (calf thymus DNA; Bio‐Rad) after excitation at 460 nm in a Cary Eclipse UV/visible spectrophotometer (Agilent Technologies).…”

Section: Methodsmentioning

confidence: 97%

See 1 more Smart Citation

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

et al. 2018

View full text Add to dashboard Cite

High occurrence of Fusarium poae (FP) and Fusarium langsethiae (FL) and their mycotoxins nivalenol (NIV) and T‐2/HT‐2 have been observed in Swiss oats. Early prediction of mycotoxin levels is important for farmers and the cereal industry to minimize the risk of contaminated food and feed. Therefore, climate chamber experiments were conducted to investigate the influence of different temperatures (10, 15, 20 °C) and durations (4, 8, 12 h) at 99% relative humidity (RH) on the infection of oats with FP and FL. In addition, to discover the most susceptible period of oats, artificial FL inoculations were conducted at different growth stages. Field experiments were performed to observe the dispersal of these fungal species within the field and to investigate the weather conditions that influence the dispersal. The climate chamber experiments revealed higher contamination with NIV and T‐2/HT‐2 in the 10 °C treatments and with a prolonged humidity duration of 12 h 99% RH. Inoculations of oat plants at early (DC 61) and mid (DC 65) anthesis, led to higher FL infection and T‐2/HT‐2 accumulation in the grains compared with treatments at earlier growth stages, which might be due to an increased susceptibility during anthesis. No indication for spore dispersal was observed in the field experiments. The results obtained, together with the cropping factors that influence infection and mycotoxin production, could be used as a first step in developing forecasting models to predict the contamination of oats with the mycotoxins NIV and T‐2/HT‐2.

show abstract

Section: Methodsmentioning

confidence: 97%

“…Following amplification, the samples were cooled to 65 °C (instead of 55 °C) and then the temperature was raised to 95 °C at a ramp rate of 0.05 °C s −1 , as explained in Schöneberg et al . ().…”

Section: Methodsmentioning

confidence: 97%

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In Europe, Fusarium langsethiae Torp & Nirenberg (FL) is considered the primary producer of T-2 and HT-2 mycotoxins (T-2 + HT-2) in oat (Yli-Mattila et al 2008;Fredlund et al 2010;Edwards et al 2012;Opoku et al 2013;Imathiu et al 2013;Hietaniemi et al 2016;Hofgaard et al 2016a;Schöneberg et al 2018). F. sporotrichioides Sherb (FS) strains isolated from cereals in Europe are potent producers of T-2 + HT-2 in vitro (Langseth et al 1998;Jestoi et al 2008;Kokkonen et al 2012a;Nazari et al 2016), but they have not been shown to be important producers of T-2 + HT-2 in oat fields.…”

Section: Introductionmentioning

confidence: 99%

“…Nivalenol (NIV), currently less important, yet considered a notable mycotoxin, which is mainly produced by F. poae (Peck) Wollenw. (FP) (Pettersson et al 1995;Yli-Mattila et al 2008;Bernhoft et al 2010;Fredlund et al 2013;Martin et al 2018;Schöneberg et al 2018). The most comprehensive data on toxin contamination in cereals has been collected by the cereal industry, but is not publicly available.…”

Section: Introductionmentioning

confidence: 99%

Contrasting responses of T-2, HT-2 and DON mycotoxins and Fusarium species in oat to climate, weather, tillage and cereal intensity

et al. 2019

View full text Add to dashboard Cite

Analysis of survey data from 804 spring-oat fields divided into five climatic regions suggested that low inoculum level of Fusarium langsethiae was a major limiter of T-2 + HT-2. A 30-year climate with a cool and rainy 10-day period preceding estimated mid-anthesis and a warm 3-week period following anthesis were positively associated with T-2 + HT-2 contamination. In 12 survey years, warm weather from 4 weeks before GS65 until harvesting increased T-2 + HT-2, except in the 1-2 weeks preceding mid-anthesis, when the requirement for high humidity dominated. F. langsethiae and F. sporotrichioides were consistently promoted by high temperature from mid-anthesis onwards. A positive response of T-2 + HT-2 to high humidity peaked 5-10 days earlier and was shorter and weaker than the responses of DON and F. graminearum. The probability of high concentration and regional mean concentration of T-2 + HT-2 tended to be lower under ploughing than under nonploughing, and they tended to increase with increasing cereal intensity. T-2 + HT-2 was positively associated with zero tillage when compared with minimum tillage. The responses of F. langsethiae and F. sporotrichioides to ploughing and cereal intensity varied by region. In contrast to T-2 + HT-2, DON was the same or higher under ploughing than under non-ploughing, not consistently affected by cereal intensity and not associated with zero tillage. F. graminearum was consistently more common under ploughing than under non-ploughing, and decreased with increasing cereal intensity in four regions. Also, F. culmorum and F. poae tended to be more common under ploughing. F. culmorum increased with cereal intensity in three regions.

show abstract

“…The extraction was done using the NucleoSpin ® 96 Plant II Kit (Macherey-Nagel, Düren, Germany) and measurement of the DNA concentration was done by measuring the emission of the fluorescent PicoGreen ® (Promega, Madison, USA) as described in Schöneberg et al (2018). Due to the high amounts of FG DNA, the extracted samples were diluted 1:200 to be within the range of the standard curve.…”

Section: Quantification Of Fusarium Graminearum By Quantitative Pcrmentioning

confidence: 99%

Infection conditions of Fusarium graminearum in barley are variety specific and different from those in wheat

et al. 2018

Self Cite

View full text Add to dashboard Cite

Fusarium head blight is one of the most noxious cereal diseases. Worldwide, F. graminearum (FG) and the mycotoxin deoxynivalenol (DON) is the most dominant species/mycotoxin in barley and wheat. Barley is often produced as on farm feed and thus routine mycotoxin analyses similar to those of cereals for human consumption are not performed. Hence, an early prediction of mycotoxin levels is important for farmers to minimise the risk of contaminated feed but also of contaminated cereals entering the cereal supply chain. Therefore, climate chamber experiments with artificial FG infection of barley investigating the influence of different temperatures (10°C, 15°C, 20°C) and durations (4 h, 8 h, 12 h) at 99% relative humidity were conducted to accumulate data to develop a forecasting system. An up to three times higher DON contamination in the 15°C treatments for the feed barley variety Ascona was detected compared with the 10°C and 20°C treatments. For the malting barley variety Concerto, the prolonged humidity durations had a stronger effect under all tested temperatures and resulted in up to two times higher DON contaminations. In addition, field experiments where spore deposition during anthesis as well as disease incidence, fungal amount and mycotoxins were observed, showed that the overwintered straw treatment resulted, depending on the year, in a three times higher FG incidence and DON content compared with the control and freshly inoculated straw treatment.

show abstract

Occurrence of Fusarium species and mycotoxins in Swiss oats—Impact of cropping factors

Cited by 61 publications

References 38 publications

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

Contrasting responses of T-2, HT-2 and DON mycotoxins and Fusarium species in oat to climate, weather, tillage and cereal intensity

Infection conditions of Fusarium graminearum in barley are variety specific and different from those in wheat

Contact Info

Product

Resources

About