Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria

Inwezerua, C; Mendonça, Nuno; Calhau, Vera; Domingues, Sara; Adeleke, O E; Silva, Gabriela

doi:10.3855/jidc.3430

Cited by 35 publications

(38 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This prevalence rate was slightly higher than a study in Switzerland which reported 8.4% among calves in a slaughter house, but in contrast to a study in South-West Nigeria which reported an absence of ESBL genes among isolates from bovine fecal specimens from slaughter houses (Reist et al, 2013;Inwezerua et al, 2014). The observation shows that the prevalence of ESBL producers in animals is on the rise and may pose risk to humans (Chantziaras et al, 2014).…”

Section: Discussioncontrasting

confidence: 54%

See 1 more Smart Citation

Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Ogefere¹,

Agbe²,

Ibadin³

2017

Not Sci Biol

View full text Add to dashboard Cite

This study was carried out to determine the prevalence of extended spectrum beta-lactamase (ESBL) among Gram negative bacteria isolated from cattle feces in Benin City, Nigeria. A total of 250 Gram negative bacteria isolates were recovered from cattle feces and were processed microbiologically using standard techniques. Emergent colonies were identified and antibacterial susceptibility tests were determined using Kirby-Bauer disk diffusion method. All bacterial isolates were screened for the presence of ESBL using the double-disc synergy method. A total of 37 (14.8%) isolates were positive for ESBL, with 33 (13.2%) indicated by ceftazidime, while only 4 (1.6%) were indicated by both ceftazidime and cefotaxime (P < 0.0001). Of the Gram negative bacterial isolates recovered, Salmonella species was the most prevalent ESBL-producer with 55.0% prevalence (P = 0.0092), while no isolate of Pseudomonas aeruginosa produced ESBL. ESBL-positive isolates showed poor susceptibility to the tested antibacterial agents in comparison with non-ESBL-producers and imipenem was the most active antibiotic. The prevalence of ESBL among Gram negative bacilli recovered from cattle feces was 14.8%. The study advises prudent use of antibiotics in the treatment of cattle and harps on improved hygiene in managing cattle, as they are potential reservoirs of ESBL-producing organisms.

show abstract

Section: Discussioncontrasting

confidence: 54%

“…In Nigeria, a previous study in Ibadan, South-Western Nigeria, detected no ESBL-producing isolate in the feces of healthy cattle (Inwezerua et al, 2014). ESBL-producing isolates have however been isolated from pigs (24.2%) and chicken…”

Section: Introductionmentioning

confidence: 99%

Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Ogefere¹,

Agbe²,

Ibadin³

2017

Not Sci Biol

View full text Add to dashboard Cite

show abstract

“…E. coli ST131 virulenceassociated clonal group was detected in the present study with similar MDR patterns, suggesting the possibility of clonal spread of these MDR E. coli. The presence of E. coli ST131 isolates in West Africa was reported by Aibinu et al [21] from hospital infections in Lagos, while one other E. coli ST131 was identified from a study of inpatients from Oyo State, Nigeria [36]. In a report from another African country, approximately 45% of extended-spectrum β-lactamase (ESBL)-producing E. coli isolates were ST131, which suggested a high rate of virulence-associated E. coli in circulation in Cape Town [75].…”

Section: Discussionmentioning

confidence: 87%

“…Earlier studies from clinical samples on antimicrobial resistance profiles of E. coli from Nigeria recorded a high prevalence to commonly administered antibiotics such as amoxicillin, amoxicillin-clavulanic acid, tetracycline, erythromycin, and trimethoprim/sulfamethoxazole [32][33][34][35]. However, few studies have assessed the antimicrobial and MDR profile of E. coli from community isolates in West Africa [36,37]. Most available data are specific to pathogenic E. coli isolates [13,38].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and multidrug resistance of Escherichia coli from community-acquired infections in Lagos, Nigeria

Adenipekun

Jackson

Ramadan

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The emergence of multidrug resistance (MDR; resistance to ≥ 2 more antimicrobials) in Escherichia coli is of concern due to complications encountered in treatment. Methodology: In this study, prevalence, antimicrobial resistance, and genetic characteristics of MDR community isolates of E. coli from Lagos, Nigeria were determined. Urine and stool samples were obtained from outpatients attending Lagos State hospitals and from animal handlers in abattoirs, poultries, and open markets, from December 2012 to July 2013.Results: Approximately 50% of urine (200/394) and 88% of stool samples (120/136) were positive for E. coli. Based upon β-lactamase production, a subset of those isolates was selected for further study. Of the 22 antimicrobials tested, E. coli exhibited resistance to all antimicrobials except amikacin and piperacillin/tazobactam. The highest levels of resistance were to tetracycline (182/247; 73.7%), trimethoprim/sulfamethoxazole (152/247; 61.5%), and ampicillin (147/247; 59.1%). Resistance to the cephalosporins ranged from 1.6%-15% including the third-and fourth-generation cephalosporins, cefpodoxime (20/247; 8.1%) and cefepime (4/247; 1.6%), respectively. MDR was observed in 69.6% (172/247) of the isolates. Forty-eight E. coli resistant to at least five antimicrobials were selected for further analysis using pulsed-field gel electrophoresis; seven distinct clusters were observed among the diverse patterns. Of the 48 MDR E. coli, 30 different sequence types (ST) were detected using multilocus sequence typing, including four ST131. Conclusions: This study demonstrated circulating MDR E. coli in the Nigerian community. Monitoring of antimicrobial resistance in developing countries is necessary to optimize empiric treatment and the prudent use of antimicrobials.

show abstract

“…No single report regarding the presence of ESBL-resistant enterobacteria in companion animals in Nigeria, exist in the literature whereas most Nigerian households keep dogs as pets, guard and/or hunting dogs. Reports in the literature which assessed animals as reservoirs of ESBL-resistant organisms in Nigeria, were those from food animals (Chah and Oboegbulem, 2007;Eze et al, 2013;Duru et al, 2013;Inwezerua et al, 2014;Olowe et al, 2015;Torres et al, 2015). This may paint a picture that only food animals are reservoirs of ESBL-resistant organisms in Nigeria.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Anyanwu¹,

Ugwu²,

EZEKWELU³

et al. 2017

Not Sci Biol

View full text Add to dashboard Cite

This study was conducted to isolate generic extended-spectrum β-lactam-resistant enterobacteria from household dogs in Nigeria, and to determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 100, randomly selected, apparently healthy household dogs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 4 µg/ml of ceftazidime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 27 ESBL-resistant enterobacterial isolates, 40.7% were Escherichia coli, 37% were Klebsiella species, 18.5% were Salmonella species, while 3.7% was Proteus species. Resistance of the E. coli isolates was 81.8% to ampicillin, 27.2% to streptomycin, 54.5% to ciprofloxacin and tetracycline, 45.4% to enrofloxacin, 90.9% to sulphamethoxazole/trimethoprim, 9.1% to amoxicillin/clavulanic acid and 0% resistant to gentamicin. Resistance of the Klebsiella isolates was 80% to ampicillin, 20% to streptomycin and amoxicillin/clavulanic acid, 30% to ciprofloxacin and enrofloxacin, 60% to tetracycline, 90% to sulphamethoxazole/trimethoprim and 10% to gentamicin. Resistance of the Salmonella isolates was 100% to sulphamethoxazole/trimethoprim, 80% to gentamicin and ampicillin, 60% to streptomycin and tetracycline, 20% to amoxicillin/clavulanic acid, and 0% to ciprofloxacin and enrofloxacin. The Proteus isolate was resistant to streptomycin and gentamycin. All the isolates were resistant to ceftazidime and cefotaxime. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 81.8% for E. coli, 70% for Klebsiella and 100% for Salmonella, respectively. This study has shown that household dogs in Nigeria, are colonized by ESBL-resistant Enterobacteriaceae and are potential reservoirs and disseminators of these organisms.

show abstract

Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria

Cited by 35 publications

References 19 publications

Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

Prevalence and multidrug resistance of Escherichia coli from community-acquired infections in Lagos, Nigeria

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Contact Info

Product

Resources

About