Occurrence of erythromycin residues in sheep milk. Validation of an analytical method

García-Mayor, M.A.; González, Gema Paniagua; Soledad-Rodríguez, Beatriz; Martínez, Rosa María Goig; Fernández-Hernando, P.; Durand-Alegría, J.S.

doi:10.1016/j.fct.2014.12.020

Cited by 18 publications

(5 citation statements)

References 13 publications

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“…Ueno Eiji et al [23] developed a method of detecting spinosad in vegetables and fruits by HPLC with UV. García-Mayor M. A. et al [24] developed a simple and fast method for the determination of erythromycin residues in sheep milk by HPLC and DAD. These methods required each target to have strong ultraviolet absorption.…”

Section: F I G U R Ementioning

confidence: 99%

Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry

Wang

Ling

Zhou

et al. 2020

J of Separation Science

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An analytical method for the determination of six emerging derivatives or metabolites together with 25 common macrolides antibiotics in milk by ultraperformance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry was established. The samples were purified with optimized Quick, Easy, Cheap, Effective, Rugged, Safe methods. The amounts of primarysecondary amine, C18, and sodium acetate adsorbent materials were optimized by response surface method to obtain the best purification effect. The chromatographic separation was carried out using the XBridge-C18 (2.1 × 100 mm, 3.5 µm, Waters) column with mobile phase of acetonitrile with 0.1% v/v formic acid-water solutions (containing 10 mmol/L ammonium acetate), separated by gradient elution. The instrument was operated in the detection mode of electrospray positive and negative ions with Full MS/data dependent MS 2 acquisition mode detection, external standard method was used for quantitative analysis. The limits of detection and limits of quantitation of 31 compounds were 0.1-0.5 µg/L and 0.5-2.0 µg/L, respectively. A total of 31 compounds performed a good linearity in the range of 1 to 200 µg/L, and the correlation coefficient was greater than 0.990. The spiked recoveries in milk samples were 81.07-110.1% and the relative standard deviation was less than 5.1%. The method was successful applied to actual sample testing in the market.

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Section: F I G U R Ementioning

confidence: 99%

Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry

Wang

Ling

Zhou

et al. 2020

J of Separation Science

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“…A literature review reveals numerous analytical methods for JOS analysis including microbiological assay (Bergan et al, 1972), thin layer chromatography (European Pharmacopoeia, 2010), spectrophotometry (Alarfaj et al, 2011), voltammetry (Belal et al, 2002), and capillary zone electrophoresis (Lalloo et al, 1997;Deng et al, 2007;Liu et al, 2010). High-performance liquid chromatography coupled to mass spectrometry (Govaerts et al, 2004;Dickson et al, 2014;Daidone et al, 2008;Van den Bossche et al, 2013), fluorescence detection (Tod et al, 1992), electrochemical detection (Gonzalez de la Huebra et al, 2007) and UV detection (Garcia-Mayor et al, 2015;Garcia-Mayor et al, 2006;Leal et al, 2001;Kanfer et al, 1998;Arsic et al, 2018) have been used to specifically investigate unchanged JOS. Nevertheless, the majority of these methods illustrate the investigation of JOS in biological fluids.…”

Section: Figurementioning

confidence: 99%

Validated Microbiological Assay for Josamycin Determination in Its Pharmaceutical Formulations

Mahmoudi

2020

J microb biotech food sci

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A simple, inexpensive and efficient agar diffusion bioassay, applying cylinder plate method, for determination of josamycin (JOS) in pure and pharmaceutical dosage forms was studied. Using a strain of Micrococcus luteus ATCC 9341 as the test organism, JOS at concentrations ranging from 0.1 to 0.5 µg/mL could be measured. The calibration graph was linear with good correlation coefficient (r = 0.999), and the method validation showed that it was specific and robust. The interference effects of degradation products and excipients were investigated in order to assess the method selectivity. The proposed method was successfully applied to estimate JOS content in tablets and suspension with good precision (intra-day RSD=0.87%; inter-day RSD=1.25%) and accuracy; the recovery values were between 97.74 and 101.13%. Bioassay was correlated with UV spectrophotometric and high performance liquid chromatographic techniques. The results obtained by these three methods were statistically analyzed by analysis of variance (ANOVA), which indicated that there was no significant difference among these methods. Therefore, we recommend this microbiological assay for drug analysis in routine quality control of josamycin in pharmaceuticals as an alternative to the physicochemical methods reported in the literature.

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“…In comparison with the respective NIP, binding specificity study between ERY, SPI, roxithromycin, josamycin, ivermectin and tylosin hemitartrate showed MIP selectivity over ERY with recoveries higher than 98% for ERY and between (15–35)% SPI, josamycin, ivermectin and tylosin hemitartrate. The developed MIPs were applied for more than 200 extraction rounds without any performance and stability drop [ 84 ].…”

Section: Mip Applications For the Extraction Of Antibiotics In Milmentioning

confidence: 99%

Molecularly Imprinted Polymers as Extracting Media for the Chromatographic Determination of Antibiotics in Milk

Bitas

Samanidou

2018

Molecules

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Milk-producing animals are typically kept stationary in overcrowded large-scale farms and in most cases under unsanitary conditions, which promotes the development of infections. In order to maintain sufficient health status among the herd or promote growth and increase production, farmers administer preventative antibiotic doses to the animals through their feed. However, many antibiotics used in cattle farms are intended for the treatment of bacterial infections in humans. This results in the development of antibiotic-resistant bacteria which pose a great risk for public health. Additionally, antibiotic residues are found in milk and dairy products, with potential toxic effects for the consumers. Hence the need of antibiotic residues monitoring in milk arises. Analytical methods were developed for the determination of antibiotics in milk, with key priority given to the analyte extraction and preconcentration step. Extraction can benefit from the production of molecularly imprinted polymers (MIPs) that can be applied as sorbents for the extraction of specific antibiotics. This review focuses on the principals of molecular imprinting technology and synthesis methods of MIPs, as well as the application of MIPs and MIPs composites for the chromatographic determination of various antibiotic categories in milk found in the recent literature.

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Occurrence of erythromycin residues in sheep milk. Validation of an analytical method

Cited by 18 publications

References 13 publications

Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry

Targeted analysis of six emerging derivatives or metabolites together with 25 common macrolides in milk using Quick, Easy, Cheap, Effective, Rugged and Safe extraction and ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry

Validated Microbiological Assay for Josamycin Determination in Its Pharmaceutical Formulations

Molecularly Imprinted Polymers as Extracting Media for the Chromatographic Determination of Antibiotics in Milk

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