Occurrence of Enterocytozoon bieneusi in Chinese Tan sheep in the Ningxia Hui Autonomous Region, China

Peng, Jun-Jie; Zou, Yang; Li, Zhixin; Liang, Qin‐Li; Song, Huwei; Li, Tao-Shan; Ma, Yuanyuan; Zhu, Xing‐Quan; Zhou, Dong‐Hui

doi:10.1007/s00436-019-06398-4

Cited by 11 publications

(4 citation statements)

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“…The PCR reaction mixture (25 µL) contained 2 µL of template, 2 µL of dNTP mixture, 2.5 µL of 10 × PCR buffer (Mg 2+ free), 1.5 mM of MgCl 2 , 0.2 µM of each primer, and 0.625 U of r- Taq DNA polymerase (TaRaKa Bio Inc., Tokyo, Japan). The conditions and cycling parameters of the primary PCR and nested PCR were performed as follows: initial denaturation for 1 min at 94 °C, followed by 35 cycles of 45 s at 94 °C for denaturation, 45 s at 55 °C for annealing, 1 min at 72 °C for extension, and a final extension at 72 °C for 10 min [ 12 ]. Positive and negative control samples were added to each PCR amplification to ensure the reliability of the result.…”

Section: Methodsmentioning

confidence: 99%

“…Group 1 and group 2 contain the zoonotic genotypes of E. bieneusi [ 6 ]. At present, BEB6 is the major genotype of E. bieneusi that has been identified from goats in Henan Province [ 8 , 9 ], Yunnan Province [ 9 , 10 ], Heilongjiang Province [ 11 ], and Ningxia Hui Autonomous Region [ 12 , 13 ]. CHG1 is the prevalent genotype of E. bieneusi in goats in Shaanxi Province [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection and Genotyping of Enterocytozoon bieneusi in Black Goats (Capra hircus) in Yunnan Province, Southwestern China

Xie

Zou

Zhao

et al. 2021

Animals

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Enterocytozoon bieneusi is a fungus-like protist that can parasitize in the intestines of humans and various animals causing a threat to public health. However, there has been no data for E. bieneusi prevalence and genotypes in black goats in Yunnan Province, Southwestern China. In this study, 907 fecal samples were collected from black goats in 5 counties from Yunnan Province. The prevalence and genotypes of E. bieneusi were examined by nested PCR amplification targeting the nuclear internal transcribed spacer (ITS). Multi-locus sequence typing (MLST) was used to further examine the potential occurrence of genetic segregation. The overall prevalence of E. bieneusi in black goats in Yunnan Province was 10.3% (93/907). Statistical analysis revealed that E. bieneusi prevalence was significantly associated with the region, age and gender of black goats (p < 0.001). Four new genotypes (CYG-1, CYG-2, CYG-3, CYG-4) and 11 known genotypes (CHG1, CHG2, CHG3, CHG5, CHG28, J, D, BEB6, Wildboar3, CD6, SDD1) of E. bieneusi were identified. At the microsatellite and minisatellite loci, 15, 2, 17, and 33 sequences were obtained, respectively, forming one new multi-locus genotype (MLG27). Phylogenetic analysis revealed that all 15 genotypes were clustered into group 1 and group 2, with zoonotic potential. This is the first report of E. bieneusi prevalence and genotypes in black goats in Yunnan Province, China. Effective control strategies and measures should be taken to reduce the risk of E. bieneusi transmission between black goats, other animals, and humans.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Genotyping of Enterocytozoon bieneusi in Black Goats (Capra hircus) in Yunnan Province, Southwestern China

Xie

Zou

Zhao

et al. 2021

Animals

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“…The phylum microsporidia consists of more than 200 microsporidian genera with more than 1500 species [ 2 , 3 ]. E. bieneusi is the most prevalent pathogen in human beings and various mammals [ 4 , 5 , 6 , 7 ], causing symptomatic and asymptomatic intestinal infections through accidental ingestion of food or water that has been contaminated with the viable spores of E. bieneusi . E. bieneusi is also deemed as one of the riskiest opportunistic pathogens for patients with HIV/AIDS [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Novel Genotypes Identification of Enterocytozoon bieneusi in Dairy Cattle in Yunnan Province, China

Song

Wang

Yang

et al. 2021

Animals

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Enterocytozoon bieneusi is a fungus-like protist parasite that can cause diarrhea and enteric diseases. The infection of E. bieneusi has been reported in many host species, including cattle and humans. However, information on prevalence and genotype distribution of E. bieneusi in dairy cattle in Yunnan province in China is still absent. In this study, 490 Holstein Cows and 351 dairy buffalo fecal samples were collected from three regions in Yunnan province, China. By using nest-PCR that targets the internal transcribed spacer (ITS), we found that the prevalence of E. bieneusi was 0.59% (5/841). DNA sequence analysis showed that five E. bieneusi genotypes were identified in this study, including two novel genotypes, YNDCEB-90 and YNDCEB-174, and three known genotypes (I, J, BEB4). Phylogenetic analysis revealed that two novel genotypes, YNDCEB-90 and YNDCEB-174, were clustered into Group 1, representing the zoonotic potential. The remaining genotypes I, J, and BEB4, which are the most frequent genotypes of E. bieneusi infection in cattle and lead to E. bieneusi infection in humans, belonged to Group 2. Although the lower prevalence of E. bieneusi was detected in dairy cattle in Yunnan province, it indicates that dairy cattle should be considered to be one of the potential hosts for transmitting E. bieneusi to humans. These findings are important for the development of effective prevention strategies for microsporidiosis.

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“…Genotyping of E. bieneusi using ITS gene sequence has been the most preferred and the gold standard method in recent decades, offering adequate information on pathogenicity and source of the organism [ 53 ]. Reportedly, BEB6, COS-1, and CHG3 of E. bieneusi have been the most prevalent genotypes among ruminants, in particular sheep and goats [ 39 , 42 , 53 ]. Of note, other less common zoonotic genotypes (Peru 6 and I), were also found in the present review, mostly isolated from humans and small ruminants [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

The global molecular epidemiology of microsporidia infection in sheep and goats with focus on Enterocytozoon bieneusi: a systematic review and meta-analysis

2021

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Background Microsporidia is a zoonotic pathogen with health consequences in immunocompromised patients. Small ruminants are a potential reservoir of microsporidia for humans in their vicinity. Hence, we aimed to evaluate the molecular prevalence of microsporidian infections with emphasis on Enterocytozoon bieneusi genotypes among sheep and goats at a global scale through systematic review and meta-analysis approach. Methods The standard protocol of preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines were followed. Eligible prevalence studies on small ruminant microsporidiosis, published from 1 January 2000 until 15 April 2021 were gathered using systematic literature search in PubMed, Scopus, Web of Science and Google Scholar databases. Inclusion and exclusion criteria were applied. The point estimates and 95% confidence intervals were calculated using a random-effects model. The variance between studies (heterogeneity) was quantified by I2 index. Results In total, 25 articles (including 34 datasets) were included for final meta-analysis. The pooled molecular prevalence of microsporidia in sheep and goats was estimated to be 17.4% (95% CI: 11.8–25%) and 16% (95% CI: 11.2–22.4%), respectively. Likewise, the overall prevalence of E. bieneusi was estimated to be 17.4% (95% CI: 11.8–25%) for sheep and 16.3% (95% CI: 11.3–22.8%) for goats. According to internal transcribed spacer (ITS) gene analysis, E. bieneusi with genotypes BEB6 (15 studies) and COS-1 (nine studies) in sheep, and CHG3 (six studies) and BEB6 (five studies) in goats were the highest reported genotypes. Conclusion The present results highlight the role of sheep and goats as reservoir hosts for human-infecting microsporidia. Therefore, this global estimate could be beneficial on preventive and control measures.

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Occurrence of Enterocytozoon bieneusi in Chinese Tan sheep in the Ningxia Hui Autonomous Region, China

Cited by 11 publications

References 40 publications

Molecular Detection and Genotyping of Enterocytozoon bieneusi in Black Goats (Capra hircus) in Yunnan Province, Southwestern China

Molecular Detection and Genotyping of Enterocytozoon bieneusi in Black Goats (Capra hircus) in Yunnan Province, Southwestern China

Prevalence and Novel Genotypes Identification of Enterocytozoon bieneusi in Dairy Cattle in Yunnan Province, China

The global molecular epidemiology of microsporidia infection in sheep and goats with focus on Enterocytozoon bieneusi: a systematic review and meta-analysis

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