Occurrence of carbapenemase-producing Enterobacteriaceae in a Portuguese river: blaNDM, blaKPC and blaGES among the detected genes

Teixeira, Pedro; Tacão, Marta; Pureza, Leide; Gonçalves, Joana; Silva, Artur M. S.; Cruz-Schneider, Maria Paula; Henriques, Isabel

doi:10.1016/j.envpol.2020.113913

Cited by 50 publications

(29 citation statements)

References 76 publications

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“…They can also promote genetic exchange between environmental and commensal bacteria in water and other environments (Ojer-Usoz et al, 2014). Different Enterobacteriaceae genera and species were found in this study, close to those found in the WWTPs of other nations, including Portugal, Colombia, and Spain (Ojer-Usoz et al, 2017;Aristizábal-Hoyos et al, 2019;Teixeira et al, 2020). These studies reported that members of the Enterobacteriaceae could survive the biological treatment processes employed in the management of municipal WWTPs.…”

Section: Total Quantification and Identification Of Enterobacteriaceae In The Wastewater Treatment Plantssupporting

confidence: 73%

The Abundance of Genes Encoding ESBL, pAmpC and Non-β-Lactam Resistance in Multidrug-Resistant Enterobacteriaceae Recovered From Wastewater Effluents

Fadare

Okoh

2021

Front. Environ. Sci.

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The aquatic environments play a critical function in the widespread of antibiotic-resistant bacteria, ultimately impacting human health. We evaluated the abundance of Enterobacteriaceae and the various resistance gene determinants in final effluents of wastewater treatment plants (WWTPs) in the Eastern Cape Province, Republic of South Africa. A total of 44 presumptive Enterobacteriaceae was recovered following standard isolation methods. Upon molecular confirmation through Matrix-Assisted Laser Desorption/Ionization Time of flight (MALDI TOF), a high predominance of Klebsiella pneumoniae (24%) was noted. All the confirmed isolates (n = 29) subjected to a panel of eighteen antibiotics categorized into eleven different classes were multidrug-resistant (MDR). They displayed resistance against antibiotics in more than three different classes. Notably, one E. cloacae exhibited resistance against all the antibiotics assayed. The multiple antibiotic resistance indices (MARI) ranged from 0.22 to 1.0, indicating an environment with high pressure of antibiotics. Conventional Polymerase Chain Reaction (PCR) analysis showed that 72.4% of the isolates harboured at least one β-lactamase genetic determinant. The most predominant extended-spectrum β-lactamases were blaCTX-M-group (21.5%), blaTEM (20%), and blaSHV (16.9%) while the most predominant plasmid-mediated AmpC-type β-lactamases were blaCIT and blaACC (25.9%) and blaEBC (22.2%). A total of 86.2% of the Enterobacteriaceae harboured a minimum of one non-β-lactam resistance gene determinant with predominance observed in catII (25%), sulII (15.8%), and sulI (14.5%). These results demonstrate an abundance of multidrug-resistant (MDR) Enterobacteriaceae in WWTPs’ effluents. The study confirms the need to optimize current WWTPs’ processes to improve the quality of effluents and ultimately reduce the potential risks of using such water when discharged into the environment.

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Section: Total Quantification and Identification Of Enterobacteriaceae In The Wastewater Treatment Plantssupporting

confidence: 73%

The Abundance of Genes Encoding ESBL, pAmpC and Non-β-Lactam Resistance in Multidrug-Resistant Enterobacteriaceae Recovered From Wastewater Effluents

Fadare

Okoh

2021

Front. Environ. Sci.

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“…Aspergillus affinis led to the largest inhibition halos, inhibiting the growth of the three-Gram negative bacteria, including the multiresistant strains K. pneumoniae and P. aeruginosa. These two multiresistant strains were isolated from the Lis River (Central Portugal) [37]. These authors highlighted that the presence of antibiotic-resistant bacteria and resistance genes in this particular environment is a major public health risk.…”

Section: Discussionmentioning

confidence: 99%

“…Three Gram-positive bacteria, Staphylococcus aureus (ATCC 6538), Kocuria rhizophila (ATCC 9341) and Enterococcus faecalis (ATCC 29212), and three Gram-negative bacteria, Escherichia coli (ATCC 25922), multiresistant Klebsiella pneumoniae (P9.5) and Pseudomonas aeruginosa (4P6.2), obtained from MicroLab from Department of Biology, University of Aveiro, Aveiro, Portugal, were used. These two multiresistant strains were isolated from the Lis River (Central Portugal) and were resistant to β-lactams and other classes of antibiotics [37]. Cultivation was done on Plate Count Agar (VWR Chemicals, Alfragide, Portugal) overnight at 37 • C. Cell suspensions were prepared using fresh bacterial colonies and turbidity adjusted to 0.5 McFarland.…”

Section: Detection Of Antibacterial Activity Based On Dual Culture Test-variantmentioning

confidence: 99%

Unveiling Biological Activities of Marine Fungi: The Effect of Sea Salt

et al. 2021

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There is an urgent need for new substances to overcome current challenges in the health sciences. Marine fungi are known producers of numerous compounds, but the manipulation of growth conditions for optimal compound production can be laborious and time-consuming. In Portugal, despite its very long coastline, there are only a few studies on marine fungi. From a collection of Portuguese marine fungi, we screened for antimicrobial, antioxidant, enzymatic, and cytotoxic activities. Mycelia aqueous extracts, obtained by high pressure-assisted extraction, and methanolic extracts of culture media showed high antioxidant, antimicrobial, and cytotoxic activities. The mycelium extracts of Cladosporium rubrum showed higher antioxidant potential compared to extracts from other fungi. Mycelia and culture media extracts of Aspergillus affinis and Penicillium lusitanum inhibited the growth of Staphylococcus aureus, Kocuria rhizophila, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, including multiresistant strains. Penicillium lusitanum and Trichoderma aestuarinum inhibited the growth of clinical strains of Candida albicans, C. glabrata, C. parapsilosis, and C. tropicalis. All extracts from culture media were cytotoxic to Vero cells. Sea salt induced alterations in the mycelium’s chemical composition, leading to different activity profiles.

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“…The presence of these key ESBL genes in clinical and environmental contexts have been previously described among Klebsiella spp. in South African and international isolates [89][90][91][92]. Since the isolates did not display full resistance to carbapenems and consequently did not harbour any of the carbapenemases genes, it was assumed that these could be kept as reserve drugs to treat infections caused by Klebsiella spp.…”

Section: Plos Onementioning

confidence: 99%

Occurrence, identification, and antibiogram signatures of selected Enterobacteriaceae from Tsomo and Tyhume rivers in the Eastern Cape Province, Republic of South Africa

2020

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The increasing occurrence of multidrug-resistant Enterobacteriaceae in clinical and environmental settings has been seen globally as a complex public health challenge, mostly in the developing nations where they significantly impact freshwater used for a variety of domestic purposes and irrigation. This paper detail the occurrence and antibiogram signatures of the Enterobacteriaceae community in Tsomo and Tyhume rivers within the Eastern Cape Province, the Republic of South Africa, using standard methods. The average distribution of the presumptive Enterobacteriaceae in the rivers ranged from 1 × 102 CFU/100ml to 1.95 × 104 CFU/100ml. We confirmed 56 (70.8%) out of 79 presumptive Enterobacteriaceae isolated being species within the family Enterobacteriaceae through the Matrix-Assisted Laser Desorption Ionization Time of Flight technique. Citrobacter-, Enterobacter-, Klebsiella species, and Escherichia coli were selected (n = 40) due to their pathogenic potentials for antibiogram profiling. The results of the antibiotic susceptibility testing gave a revelation that all the isolates were phenotypically multidrug-resistant. The resistance against ampicillin (95%), tetracycline and doxycycline (88%), and trimethoprim-sulfamethoxazole (85%) antibiotics were most prevalent. The Multiple Antibiotic Resistance indices stretched from 0.22 to 0.94, with the highest index observed in a C. freundii isolate. Molecular characterisation using the PCR technique revealed the dominance of blaTEM (30%; 12/40) among the eight groups of β-lactams resistance determinants assayed. The prevalence of others was blaCTX-M genes including group 1, 2 and 9 (27.5%), blaSHV (20%), blaOXA-1-like (10%), blaPER (2.5%), and blaVEB (0%). The frequencies of the resistance determinants for the carbapenems were blaKPC (17.6%), blaGES (11.8%), blaIMP (11.8%), blaVIM (11.8%), and blaOXA-48-like (5.9%). Out of the six plasmid-mediated AmpC (pAmpC) genes investigated blaACC, blaEBC, blaFOX, blaCIT, blaDHA, and blaMOX, only the first four were detected. In this category, the most dominant was blaEBC, with 18.4% (7/38). The prevalence of the non-β-lactamases include tetA (33.3%), tetB (30.5%), tetC (2.8%), tetD (11.1%), tetK (0%), tetM (13.9%), catI (12%), catII (68%), sulI (14.3%), sulII (22.9%) and aadA (8.3%). Notably, a C. koseri harboured 42.8% (12/28) of the genes assayed for which includes five of the ESBL genes (including the only blaPER detected in this study), two of the pAmpC resistance genes (blaACC and blaCIT), and five of the non-β-lactamase genes. This study gives the first report on C. koseri exhibiting the co-occurrence of ESBL/AmpC β-lactamase genes from the environment to the best of our knowledge. The detection of a blaPER producing Citrobacter spp. in this study is remarkable. These findings provide evidence that freshwater serves as reservoirs of antimicrobial resistance determinants, which can then be easily transferred to human beings via the food chain and water.

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Occurrence of carbapenemase-producing Enterobacteriaceae in a Portuguese river: blaNDM, blaKPC and blaGES among the detected genes

Cited by 50 publications

References 76 publications

The Abundance of Genes Encoding ESBL, pAmpC and Non-β-Lactam Resistance in Multidrug-Resistant Enterobacteriaceae Recovered From Wastewater Effluents

The Abundance of Genes Encoding ESBL, pAmpC and Non-β-Lactam Resistance in Multidrug-Resistant Enterobacteriaceae Recovered From Wastewater Effluents

Unveiling Biological Activities of Marine Fungi: The Effect of Sea Salt

Occurrence, identification, and antibiogram signatures of selected Enterobacteriaceae from Tsomo and Tyhume rivers in the Eastern Cape Province, Republic of South Africa

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