Occurrence of Campylobacter and Listeria monocytogenes in a Poultry Processing Plant

Abstract

The occurrence of Campylobacter and Listeria monocytogenes was studied in 645 samples from surfaces, water, and poultry products (chicken carcasses, chicken parts, viscera, and spoils) in a poultry processing plant in southern Brazil. The automated mini-VIDAS system was used to detect the presence of Campylobacter and L. monocytogenes on the samples. The positive samples were confirmed by conventional methods. Campylobacter and L. monocytogenes were found in 16.6 and 35.6% of the analyzed samples, respectively. The sampling points with the highest Campylobacter incidence were intestine (63.3%, 19 of 30 samples), gallbladder (46.7%, 14 of 30), carcasses before evisceration (33.33%, 10 of 30), and carcasses after plucking (30%, 9 of 30). For L. monocytogenes, the majority of positive samples were from frozen breast (100%, 15 of 15 samples), frozen wing (93.3%, 14 of 15), fresh breast (83.3%, 25 of 30), fresh wing (80%, 24 of 30), skin of breast and leg (76.7%, 23 of 30), frozen leg (60%, 9 of 15), and fresh leg (50%, 15 of 30).

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

“…The MiniVIDAS CAM method has been successful for the detection of Campylobacter spp. in tissue samples (tonsils and lymph nodes) and fecal material from pigs during processing (Nesbakken et al, 2003), in a variety of chicken parts with positive results confirmed by culture methods (Reiter et al, 2005), and in artificiallycontaminated ground beef and fresh cut vegetables (Chon et al, 2011). Our experiences (OAO) with the MiniVIDAS for screening poultry meat are in accordance with the majority of reports mentioned above.…”

Phenotypic and Genotypic Characterization of Listeria Species Isolated From Chicken and Milk Products.

“…The most important aspect in food hygiene is the ability of the bacteria to survive in a wide range of temperatures and to make biofilms on various environmental surfaces, which serve as natural habitats or reservoirs (Duggan and Phillips, 1998). Listeria monocytogenes wasn't found in Frozen wings of poultry, frozen breast of poultry that totally disagreed with (Reiter et al, 2005) The result of in vitro sensitivity test showed that ,the isolated L.monocytogenes were highly sensitive to Sulphamethoxazole /trimethoprim (100%), so we can use it in its medication (Swaminathan and Gerner-Smidt, 2007),followed by Gentamycin Ceftazidime and Cephazoline (71.4%), followed by Sulphamethoxazole, ceftriaxone (42.8%), weak sensitivity to Penicillin (14.2%), while the isolated strains were resistant to Erythromycin, Ampicillin and Clindamycin and these results came in accordance with those recorded by (Zeinali et al, 2017) and disagreed with (Altuntas et al,2012). All L.monocytogenes isolates were positive to CAMP test and showed narrow zone of β-heamolysis in sheep agar, the similar results were reported by (Marrouf et al ., 2007).…”

Listeria monocytogenes: monitoramento desse perigo biológico na cadeia produtiva de frangos do sul do Rio Grande do Sul

“…O mesmo se verifica quando se compara os dados obtidos neste estudo com outras pesquisas realizadas no Brasil. Chiarini (2007) avaliou duas plantas de processamento de aves no interior de São Paulo e encontrou ocorrência de 20,1% em abatedouros com evisceração automática e 16,4% naqueles com evisceração manual, e Reiter et al (2005) encontraram o patógeno em 35,6% das amostras provenientes de uma linha de abatedouro de aves no Rio Grande do Sul. É interessante frisar que embora as coletas tenham sido realizadas em três fases do processo de abate (início, fase intermediária e fase final do abate), não houve diferença na taxa de isolamento de L. monocytogenes entre as distintas fases (dados não mostrados), contrapondo ao descrito por Whyte et al (2004), que encontraram grande interferência do horário de amostragem durante o abate de frangos sobre a frequência de amostras positivas para esse microrganismo.…”

“…O fato de não ter havido isolamento de L. monocytogenes nas aves que entraram na planta de processamento (plataforma de desembarque e sangria), com posterior presença do patógeno no produto final, demonstra a dispersão desse microrganismo e, portanto, a importância da contaminação cruzada dentro dessa indústria, fato corroborado pelo frequente número de pontos em que esse microrganismo foi isolado e pela prevalência de apenas dois sorotipos (1/2b e 4b) nas amostras provenientes do abatedouro. Pode-se observar pela Tabela 1, que à medida que o processamento progride, aumenta a taxa de contaminação por L. monocytogenes, o que também foi relatado por Reiter et al (2005), os quais descrevem que a contaminação das carcaças ocorre principalmente durante ou após as etapas de evisceração e resfriamento, e por Barbalho et al (2005), que avaliaram um abatedouro de frangos na Bahia e encontraram L. monocytogenes em 14,3% das carcaças já embaladas, as quais não estavam contaminadas nas etapas anteriores.…”