Occurrence of Beko Disease Caused by Microsporidium seriolae (Microspora) in Hatchery-Reared Juvenile Yellowtail.

Sano, Motohiko; Sato, Jun; Yokoyama, Hiroshi

doi:10.3147/jsfp.33.11

Cited by 13 publications

(10 citation statements)

References 6 publications

(4 reference statements)

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“…challenges to newly stocked fish in the Kyushu region have continued yearly. Monitoring over an extended period has revealed no other microsporidian infections in these fish (Yokoyama et al 1996, Sano et al 1998. Under such conditions the highly sensitive nested PCR may be used in preference to the single PCR assay for infection screening.…”

Section: Discussionmentioning

confidence: 99%

“…The causative pathogen could not be classified into any established genus and Egusa (1982) placed it provisionally into the collective genus A4icrosporidium Balbiani, 1884, as M. seriolae. This parasite has continued to cause high mortalities among cultured yellowtail and severely affects seed production in the Kyushu region (Yokoyama et al 1996, Sano et al 1998.…”

Section: Introductionmentioning

confidence: 99%

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Single and nested polymerase chain reaction assays for the detection of Microsporidium seriolae (Microspora), the causative agent of 'Beko' disease in yellowtail Seriola quinqueradiata

Bell¹,

Yokoyama²,

Aoki³

et al. 1999

Dis. Aquat. Org.

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Single and nested polymerase chain reaction (PCR) assays were developed for the detection of the microsporidian parasite Microsporidium senolae, which is responsible for emaciation and even death in farmed Japanese~yellowtall. Extremely high rDNA identities exist between this parasite and other members of the as yet unclassified genus, necessitating the design of generic, rather than specles-specific primer sets. The nested PCK was several orders of magnitude more sensitive than the standard single PCRs, with visible target product ampMied from as little as 0.01 pg of parasite DNA (equivalent to that extracted from a single spore). The specificity of the assays was tested against a range of potential host fishes and 6 other microsporidians infecting either fish or the musculature of their hosts. Single PCRs were found to be specific to the target genus, but the nested PCR replicated rDNA from several different microsporidian genera, limiting its utility. This study highlights problems associated with the use of the rRNA gene for PCR assays of certain microsporidians, but nevertheless provides a rapid and sensitive means for the detection of pre-spore forms not possible by current staining methods. Consequently, these assays may be employed for further studies on the portals of entry. migration to the musculature and transmission of this economically important pathogen.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single and nested polymerase chain reaction assays for the detection of Microsporidium seriolae (Microspora), the causative agent of 'Beko' disease in yellowtail Seriola quinqueradiata

Bell¹,

Yokoyama²,

Aoki³

et al. 1999

Dis. Aquat. Org.

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“…Until recently, all of the few known Kabatana species had been reported from eastern Asia (Lom 2002). Kabatana arthuri (Lom, Dyková & Shaharom, 1990) is recorded from the trunk musculature of the freshwater catfish Pangasius sutchi in Thailand (Dyková & Lom 2000), K. seriolae (Egusa, 1982) from the trunk muscles of yellowtail Seriola quinqueradiata in Japan (Sano et al 1998), andK. takedai (Awakura, 1974) from the heart, trunk and other muscles of freshwater salmonids in Japan and far eastern Russia (Lom et al 2001).…”

Section: Introductionmentioning

confidence: 99%

First record of a Kabatana sp. microsporidium infecting fish in the Atlantic Ocean

Barber

Davies²,

Ironside³

et al. 2009

Dis. Aquat. Org.

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Two-spotted goby Gobiusculus flavescens from the Swedish Gullmarsfjord regularly present subcutaneous creamy-white patches in the body musculature, associated with Kabatana sp. infection. Analysis of the 16S rRNA gene of the microsporidium showed 98.54% homology with Kabatana newberryi infecting a marine goby from California, indicating that the Swedish microsporidium is either a different strain of K. newberryi or a closely related species. This represents the first record of a Kabatana species in the Atlantic Ocean. The genetic similarity of the 2 microsporidia was paralleled by close infection phenotypes. Infected muscle fibres were swollen compared to adjacent non-infected fibres, and mature spore masses were found throughout the skeletal musculature. No xenoma formation was detected. Since G. flavescens is an established model species in behavioural ecology, the host -parasite system is ideally suited for testing how microsporidian infections affect host behaviour and fitness.

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“…RSB, and M. seriolae) are conspecific, experimental infection trials will lead to a decisive results. However, artificial transmission using M. seriolae spores has failed, suggesting involvement of the intermediate host in the life cycle (Sano et al, 1998). Instead, exposure of uninfected fish to the enzootic waters for each microsporidian may be designed as an alternative experimental method.…”

Section: Discussionmentioning

confidence: 99%

“…in red sea bream Pagrus major (Egusa, 1982;Egusa et al, 1988). It has been known that epizootics of 'beko' disease frequently occurred in cultured yellowtail in Kyushu (Sano et al, 1998). Although the host range for M. seriolae and Microsporidium sp.…”

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confidence: 99%

Microsporidian Infection in the Trunk Muscle of Hatchery-bred Juvenile Spotted Halibut Verasper variegatus

Yokoyama

Zhang

et al. 2008

Fish Pathol.

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ABSTRACT-Recently, a microsporidian infection was found in the trunk muscle of hatchery-bred juvenile spotted halibut Verasper variegatus. The disease occurred from mid-July to the end of September 2007 and the cumulative mortality reached approximately 20%. Infected fish showed the external sign of a concave body surface on the eyed side, and the microsporidian parasite formed numerous 'cysts' in the muscle. These characteristics resembled 'beko' disease caused by Microsporidium seriolae from cultured yellowtail Seriola quinqueradiata, Microsporidium sp. from red sea bream Pagrus major (RSB) and Microsporidium sp. from gilthead sea bream Sparus aurata (GSB), but several differences were observed in associated pathological findings such as internal hemorrhage around the 'cysts'. Average dimensions of spores from spotted halibut (SH) were 3.07 × 2.13 µm, which were relatively smaller than those of M. seriolae, Microsporidium sp. RSB and Microsporidium sp. GSB, but the ranges of measurements overlapped among them. Molecular analysis of rDNA sequences suggested that the present parasite, provisionally named as Microsporidium sp. SH, was distinct from the other known species.

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Occurrence of Beko Disease Caused by Microsporidium seriolae (Microspora) in Hatchery-Reared Juvenile Yellowtail.

Cited by 13 publications

References 6 publications

Single and nested polymerase chain reaction assays for the detection of Microsporidium seriolae (Microspora), the causative agent of 'Beko' disease in yellowtail Seriola quinqueradiata

Single and nested polymerase chain reaction assays for the detection of Microsporidium seriolae (Microspora), the causative agent of 'Beko' disease in yellowtail Seriola quinqueradiata

First record of a Kabatana sp. microsporidium infecting fish in the Atlantic Ocean

Microsporidian Infection in the Trunk Muscle of Hatchery-bred Juvenile Spotted Halibut Verasper variegatus

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