Occurrence of anti-Neospora caninum antibodies in sheep from farms located in northern Parana, Brazil

Munhoz, K.; Neto, Mario de Luca; Santos, Sabrina Martins dos; Garcia, João Luís; Guimarães, José da Silva; Vidotto, Odilon; Headley, Selwyn Arlington; Yamamura, Milton Hissashi

doi:10.5433/1679-0359.2010v31n4p1031

Cited by 10 publications

(9 citation statements)

References 22 publications

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“…Otero et al (2005) Munhóz et al (2010), no Paraná (81,82%), no entanto esses autores trabalharam com um número inferior de propriedades que a do presente estudo, sendo seis, trinta e onze respectivamente. Entre os ovinos de Sergipe pesquisados, os títulos de anticorpos variaram de 50 a 6400, sendo o título de 50 observado em 96,02% (459/478) dos animais positivos (Quadro 2).…”

Section: Resultsunclassified

“…Em todas as lâminas foram usados controles positivos e negativos previamente conhecidos e para detecção de anticorpos para N. caninum utilizou-se títulos com ponto de corte 50. As amostras positivas foram tituladas em diluições seriadas na base 2 (Figliuolo et al 2004, Souza Neto et al 2009, Munhóz et al 2010, Rossi et al 2011, Tembue et al 2011.…”

Section: Methodsunclassified

“…Levantamentos sorológicos para a detecção de anticorpos para N. caninum em ovinos no Brasil demonstram ampla ocorrência do agente, sendo registrado nas regiões Sul (Vogel et al 2006, Romanelli et al 2007, Pappen et al 2008, Munhóz et al 2010, Rosa et al 2011, Moura et al 2014, Paiz et al 2015, Sudeste (Figliuolo et al 2004, Rossi et al 2011, Langoni et al 2010, Salaberry et al 2010, Rizzo et al 2011, Paiz et al 2015, Centro Oeste (Oshiro et al 2008, Ueno et al 2009) e Norte (Aguiar et al 2004), com ocorrência variando de 2,93 a 59,22%.…”

Section: Introductionunclassified

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Pesquisa de anticorpos IgG para Neospora caninum e avaliação dos fatores de risco em ovinos do Estado de Sergipe

et al. 2017

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The aim of this study was to determine the occurrence of antibodies IgG against Neospora caninum and evaluate the risk factors associated with the infection in ovine herds, State of Sergipe, Brazil. Blood samples (n=1200) were collected from sheep raised in 60 sheep run located in the three mesoregions of the State of Sergipe, between 2011 e 2012. Antibodies were investigated by Indirect Immunofluorescence Antibody Test of which cutoff point was 50 and positive samples were diluted in base 2 until the last positive titer. Data from 15 variables was obtained from questionnaires given to farmers. Absolute and relative frequencies were determined and the risk factors were analyzed by Pearson's Qui-Square test (p≤0,05). The occurrences of serum reactive sheep were 39.83% (478/1200). The occurrences of positive sheep and sheep run were 55.88% (380/680) and 88.24% (30/34) in the Eastern region; 21.42% (60/280) e 42.85% (6/14) in dry region and 15.83% (38/240) e 41.67% (5/12) in the backwoods respectively. Antibody titers ranged from 50 (n=459), represented 96.02% (459/478) of seropositive samples to 6400 (1/478). Among the significant variables in the multivariate analysis were considered risk factors for infection with N. caninum were, sheep run located in Eastern region (p=0.000, OR=4.64, CI95%=3.36-6.41); standing and running water sources (p=0.000 OR=2.03, CI95%=1.41-2.92), absence of quarantine (p=0.000, OR=2.71, CI95%=2.08-3.53), absence of dunghill (p=0.000, OR=3.14, CI95%=2.45-4.02), presence of dogs (p=0.000, OR=2.74, CI95%=1.73-433), presence of wild animals (p=0.000, OR=3.45, CI95%=2.44-4.87) and subsistence (p=0.000, OR=4.99, CI95%=3.15-7.92) or reproduction (p=0.002, OR=1.74, CI95%=1.22-2.49) livestock were important risk factors. Our results highlight the occurrence of N. caninum in the ovine herds from State of Sergipe. Management and location of sheep runs were important risk factors associated to the infection.INDEX TERMS: Neospora caninum, infection, parasite, sheep, risk factors, indirect fluorescent antibody test.

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Section: Resultsunclassified

Section: Methodsunclassified

Section: Introductionunclassified

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Pesquisa de anticorpos IgG para Neospora caninum e avaliação dos fatores de risco em ovinos do Estado de Sergipe

et al. 2017

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“…Some studies in Brazil have reported low percentages of seropositive sheep with titers ≥ 50, as 8.1% (SALABERRY et al, 2010) in Minas Gerais, 9.2% (FIGLIUOLO et al, 2004) and 13.9% (MUNHÓZ et al, 2010) in São Paulo and 9.5% (ROMANELLI et al, 2007) in Paraná. In this study, considering as cut-off the titer of 50, the seropositivity decrease to 37.58% (224/596; 95%CI 33.79-41.45%), but stills remains higher than the other studies cited above.…”

Section: Discussionmentioning

confidence: 99%

Antibodies to Neospora caninum in sheep from slaughterhouses in the state of São Paulo, Brazil

Paiz

Silva

Menozzi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Neosporosis is an emergent disease responsible for considerable economic impact due to reproductive losses. Its zoonotic potential remains unknown. This study involved a survey of antibodies to Neospora caninum in slaughtered sheep and their association with epidemiological variables. Serum samples from 596 sheep from the states of São Paulo and Rio Grande do Sul, Brazil, were collected in two slaughterhouses located in São Paulo and evaluated by indirect fluorescence antibody test (IFAT), using cut-off titers of 25. Among these samples, 353/596 (59.23%; 95%CI 55.23-63.10) were positive and 263/353 (74.50%; 95%CI 69.71-78.77%) were from Rio Grande do Sul. Statistical associations were determined in the univariate analysis between the serological results and sex, breed and municipality of origin. Sheep that came from extensive breeding system showed higher chance (OR=2.09) of presenting antibodies to N. caninum in relation to those from semi-intensive system. Higher chance was also observed for the different studied breeds, except Bergamácia, in relation to Hampshire Down. The results revealed the presence of infection by N. caninum in sheep from slaughterhouses.Keywords: Neospora caninum, serology, sheep, slaughterhouses. ResumoA neosporose é uma doença emergente responsável por considerável impacto econômico devido a perdas reprodutivas e seu potencial zoonótico permanece desconhecido. Este estudo envolveu a pesquisa de anticorpos para Neospora caninum em ovinos abatidos e sua associação com variáveis epidemiológicas. Amostras de soro sanguíneo de 596 ovinos, procedentes dos estados de São Paulo e Rio Grande do Sul, Brasil, foram colhidas em dois abatedouros localizados em São Paulo, e avaliadas pelo teste de reação de imunofluorescência indireta (RIFI), utilizando-se como ponto de corte o título de 25. Dentre essas amostras, 353/596 (59,23%; IC95% 55,23-63,10) foram positivas e dentre os ovinos positivos 263/353 (74,50%; IC95% 69,[71][72][73][74][75][76][77][78]77%) eram procedentes do Rio Grande do Sul. Associações estatísticas foram determinadas na análise univariada entre os resultados sorológicos e o sexo, raça e município de origem. Ovinos oriundos de sistema de criação extensivo demonstraram maior chance (OR=2.09) de apresentarem anticorpos para N. caninum em relação aos de sistema semi-intensivo. Uma maior chance também foi observada para as diferentes raças estudadas, exceto Bergamácia, em relação à raça Hampshire Down. Os resultados revelaram a presença de infecção por N. caninum em ovinos de abatedouros.

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“…The risk factors for this species are not widely known, but some studies have identified a relationship between seropositivity and property size, ingestion of backwater, animal slaughter on the property, viscera exposed to dogs and the presence of reproductive problems (Faria et al, 2010;Machado et al, 2011;Munhóz et al, 2010). Considering the relevance of this disease and the scarcity of epidemiological information in Sergipe, the aim of this study was to identify the main factors related to the frequency of antibodies against N. caninum in a sheep flock from the State of Sergipe.…”

Section: Introductionmentioning

confidence: 99%

Factors associated with the seroprevalence of Neospora caninum (Apicomplexa: Toxoplasmatinae) in sheep from the State of Sergipe, Brazil

et al. 2019

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The aim of this study was to determine the prevalence and factors associated with the occurrence of antibodies against Neospora caninum in sheep from Sergipe, northeastern Brazil. A total of 932 sheep serum samples from 54 properties in 19 municipalities from the State of Sergipe, Brazil were collected and assayed using an indirect fluorescent antibody test (IFAT) to assess antibodies against N. caninum. A cutoff point of 1:50 was adopted and results showed that 12.45% (116/932) of sheep were serum-reactive. Based on an unconditional logistic regression, the presence of dogs on the property was associated with protection (OR= 0.323), whereas the use of exchanged or borrowed breeding males was associated with infection (OR= 22.287). These results indicate that the occurrence of antibodies against N. caninum is endemic in the State municipalities.

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Occurrence of anti-Neospora caninum antibodies in sheep from farms located in northern Parana, Brazil

Cited by 10 publications

References 22 publications

Pesquisa de anticorpos IgG para Neospora caninum e avaliação dos fatores de risco em ovinos do Estado de Sergipe

Pesquisa de anticorpos IgG para Neospora caninum e avaliação dos fatores de risco em ovinos do Estado de Sergipe

Antibodies to Neospora caninum in sheep from slaughterhouses in the state of São Paulo, Brazil

Factors associated with the seroprevalence of Neospora caninum (Apicomplexa: Toxoplasmatinae) in sheep from the State of Sergipe, Brazil

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