Occurrence, genetic characterization and antimicrobial resistance ofSalmonellaisolated from chicken meat and giblets

Abstract

SUMMARY This study was undertaken to survey the presence of Salmonella in 200 chicken samples collected from Mansoura, Egypt. Salmonella was detected in 16% (8/50), 28% (14/50), 32% (16/50) and 60% (30/50) of whole chicken carcasses, drumsticks, livers and gizzards, respectively, with an overall prevalence of 34% (68/200) among all samples. One hundred and sixty-six isolates were identified biochemically as Salmonella, and confirmed genetically by PCR, based on the presence of invA and stn genes. The spvC gene, however, was detected in only 25.3% (42/166) of the isolates. Isolates were serotyped as Salmonella Enteritidis (37.3%), S. Typhimurium (30.1%), S. Kentucky (10.8%), S. Muenster (8.4%), S. Virchow (4.8%), S. Anatum (4.8%), S. Haifa (1.2%), and four were non-typable. Antimicrobial susceptibility tests of the Salmonella isolates revealed that 100% were resistant to each of erythromycin, penicillin, and amoxicillin, while 98.8%, 96.4%, 95.2%, and 91.6% were resistant to nalidixic acid, sulphamethoxazole, oxytetracycline, and ampicillin, respectively. Multidrug resistance was evident for 92.8% of the isolates. The high contamination level of chicken meat with multidrug-resistant Salmonella can constitute a problem for public health.

Tavuk Karkaslarından İzole Edilen Salmonella spp. ve Listeria monocytogenes Prevalansı, Antimikrobiyal Direnci ve Moleküler Karakterizasyonu

“…In the current study, 93.75% of the Salmonella isolates was classified as multidrug resistant. Our results were relatively high according to some studies [15,16] while showed similarities to others [4,6] .…”

“…ranged from 0% to 100% was reported in poultry [2,4,5] . The authors emphasize that differences of Salmonella prevalence in chicken meat could be based on the geographical differences, sampling techniques, detection methods, slaughterhouse hygiene and cross-contamination of products [5,6] . Regarding previous studies in Turkey, Salmonella spp.…”

“…Several reports and books have been published about antibiotic resistance problem and the reasons behind the increasing rates. It was highlighted that poultry meat may play a major role in transmission [4][5][6] . The inappropriate, uncontrolled and excessive use of antibiotics in the treatment of infections in humans and veterinary medicine may be the reason for high rates of resistance, in poultry [7] .…”

Isolation, identification, and antibiogram studies of Salmonella species and Escherichia coli from boiler meat in some selected areas of Bangladesh

“…Both developed and developing countries are largely affected by foodborne infections. Foodborne diseases not only affect people's health and well-being but also have an economic impact on individuals and the countries 1 while the impact in case of developing countries like Bangladesh is higher. It reduces markedly social and economic productivity of the countries.…”

DNA Profiles of Salmonella Spp. Isolated from Chicken Products and From Broiler and Human Feces

“…The serotypes Enteritidis and Typhimurium have been reported in other research studies (Abd-Elghany et al, 2015;Suresh et al, 2011;Thakur et al, 2013) as the most common in chickens. This study, however, did not detect any isolates of these serotypes in chicken product or fecal samples.…”