Occurrence and reduction of antibiotic resistance genes in conventional and advanced drinking water treatment processes

Hu, Yaru; Zhang, Tianyang; Jiang, Lei; Luo, Yi; Yao, Shijie; Zhang, Dong; Lin, Kuangfei; Cui, Changzheng

doi:10.1016/j.scitotenv.2019.03.143

Cited by 79 publications

(34 citation statements)

References 48 publications

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“…It was also observed that chlorination had no signi cant ARG removal effect (Liu et al 2019). Several ARGs (e.g., qnrB, tetM and tetW) were also increased after disinfection with sodium hypochlorite (Hu et al 2019). The increases in ARGs may be attributed to an inadequate chlorine dosage inducing the formation of more pili for conjugative transfer (Guo et al 2015).…”

Section: Removal Of Args In Different Hwwtpsmentioning

confidence: 96%

“…All runs were performed with positive standards and negative controls. The positive standards were obtained by cloning target DNA into plasmids at different dilutions, and nuclease-free water was used as the negative control as described in a previous study (Hu et al 2019). The details of the primers, annealing temperatures, and amplicons for the target genes are listed in Table A.4.…”

Section: Characterization Of Bacteria and Quanti Cation Of Target Genesmentioning

confidence: 99%

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Occurrence and Removal of Antibiotics, Antibiotic-Resistance Genes and Bacterial Communities in Hospital Wastewater

Yao

Yang

et al. 2021

Preprint

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Hospital wastewater contains a variety of human-related antibiotics and pathogens, which makes the treatment of hospital wastewater essential. However, there is a lack of research on these pollutants in hospital wastewater treatment plants. In this study, the characteristics and removal of antibiotics and antibiotic resistance genes (ARGs) in independent treatment processes of hospitals of different scales (primary hospital H1, secondary hospital H2, and tertiary hospital H3) were investigated. The occurrence of antibiotics and ARGs in wastewater from three hospitals varied greatly. The first-generation cephalosporin cefradine was detected at a concentration of 2.38 μg/L in untreated wastewater of H1, while the fourth-generation cephalosporin cefepime had the highest concentration, 540.39 μg/L, in H3. Ofloxacin was detected at a frequency of 100% and had removal efficiencies of 44.2%, 51.5%, and 81.6% for H1, H2, and H3, respectively. The highest relative abundances of the β-lactam resistance gene blaGES-1 (1.77×10-3 copies/16S rRNA), quinolone resistance gene qnrA (8.81×10-6 copies/16S rRNA), and integron intI1 (1.86×10-4 copies/16S rRNA) were detected in the treated wastewaters. The concentrations of several ARGs were increased in the treated wastewater (e.g., blaOXA-1, blaOXA-10 and blaTEM-1). Several pathogenic or opportunistic bacteria (e.g., Acinetobacter, Klebsiella, Aeromonas, and Pseudomonas) were observed with high relative abundances in the treated wastewater. These results suggested the co-occurrence of novel antibiotics, ARGs, and antibiotic-resistant pathogens in hospital wastewater that may spread into the receiving water environment.

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Section: Removal Of Args In Different Hwwtpsmentioning

confidence: 96%

Section: Characterization Of Bacteria and Quanti Cation Of Target Genesmentioning

confidence: 99%

Occurrence and Removal of Antibiotics, Antibiotic-Resistance Genes and Bacterial Communities in Hospital Wastewater

Yao

Yang

et al. 2021

Preprint

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“…Adsorption techniques are broadly practiced around the globe to treat AB-contaminated liquid waste. There are a few mechanisms involved in adsorption processes for removing the AB from the WW using adsorbents; the mechanisms are ion exchange, pore filling, and pi-pi electron interaction 82 , the electrostatic mechanism 83 , surface complexation, and hydrogen bonding. Removal using the ion-exchange mechanism depends upon the pollutant size and surface functional group (SFG) of the adsorbent used 84 .…”

Section: Removal Processes and Mechanisms Adsorptionmentioning

confidence: 99%

Proclivities for prevalence and treatment of antibiotics in the ambient water: a review

et al. 2020

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In the intial two decades of the twenty-first century, antibiotic (AB) resistance in human pathogens has emerged as a major challenge for water, sanitation, and public health. Considering these challenges, we critically reviewed AB-related studies with particular emphasis on their (i) patterns of consumption, (ii) pathway prevalences and environmental implications in ambient waters, and (iii) benefits and limitations of existing AB removal/purging techniques. We found that lifestyle, land use, urbanization, the ease of availability, and the tendency of the medical practitioners to recommend ABs are the key factors governing the AB use pathway and enrichment in the environment. In the developing world, the most prevalent group of ABs is quinolone, whereas in the developed world, older-generation AB groups are most prevalent. Further, enormous variability in the consumption of ABs around the globe is explicitly highlighted in this study. Ciprofloxacin has been reported in the highest concentration among all the ABs with 28–31 mg L−1 in the raw wastewater of the Indian subcontinent. We found that adsorption may be one of the most efficient AB removal techniques, and NaOH-activated carbon prepared from Macadamia nut shells is the most effective adsorbent identified to date. The literature showed that the Langmuir isotherm and pseudo-second-order kinetic model explain the AB adsorption mechanism most effectively. The future challenge lies in developing advanced protocols and markers to prioritize the strategy and simulate the ecotoxicities of the individual and a mixture of ABs.

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“…However, in the presence of trace tetracycline, the relative abundances of efflux pumps, such as tetA and tetG, tended to remarkably increase in the UNITANK process (Liu et al 2019). Overall, the conventional and advanced processes resulted in 0.03-2.40 log reductions for most ARG subtypes, such as intI1, qepA, strA, and strB, but neither treatment method affected the reduction of tetM (Hu et al 2019). Traditionally, a combination of physical, chemical, and biological processes has been widely used in wastewater treatment plants to remove pollutants (Li et al 2017), such as BOD 5 , COD Cr , SS, TP, TN, etc.…”

Section: Introductionmentioning

confidence: 96%

Comparison of the elimination effectiveness of tetracycline and AmpC β-lactamase resistance genes in a municipal wastewater treatment plant using four parallel processes

et al. 2020

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Municipal wastewater treatment plants (mWWTPs), considered reservoirs of antibiotic resistance genes (ARGs), are selected to compare the contributions of technology and process to ARG removal. Fifteen ARGs (tetA, tetB, tetC, tetE, tetG, tetL, tetM, tetO, tetQ, tetS, tetX, MOX, CIT, EBC, and FOX) and two integron genes (intI1, intI2) were tracked and detected in wastewater samples from a large-scale mWWTP with four parallel processes, including three biological technologies of AAO (anaerobic-anoxic-oxic), AB (adsorption-biodegradation), and UNITANK, two different disinfection technologies, and two primary sedimentation steps. The results showed that ARGs were widely detected, among which tetA and tetM had the highest detection rate at 100%. AAO was the most effective process in removing ARGs, followed by the AB and UNITANK processes, where the separation step was critical: 37.5% AmpC β-lactamase genes were reduced by the secondary clarifier. UV disinfection was more efficient than chlorination disinfection by 47.0% in ARG removal. Both disinfection and primary sedimentation processes could effectively remove integrons, and the swirling flow grit chamber was a more effective primary settling facility in total ARG removal than the aerated grit chamber. The tet genes and AmpC β-lactamase genes were significantly correlated with the water quality indexes of BOD 5 , COD Cr , SS, TP, TOC, pH and NH 4 +-N (p < 0.05). In addition, the correlation between efflux pump genes and AmpC β-lactamase genes was strongly significant (r 2 = 0.717, p < 0.01). This study provides a more powerful guide for selecting and designing treatment processes in mWWTPs with additional consideration of ARG removal.

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Occurrence and reduction of antibiotic resistance genes in conventional and advanced drinking water treatment processes

Cited by 79 publications

References 48 publications

Occurrence and Removal of Antibiotics, Antibiotic-Resistance Genes and Bacterial Communities in Hospital Wastewater

Occurrence and Removal of Antibiotics, Antibiotic-Resistance Genes and Bacterial Communities in Hospital Wastewater

Proclivities for prevalence and treatment of antibiotics in the ambient water: a review

Comparison of the elimination effectiveness of tetracycline and AmpC β-lactamase resistance genes in a municipal wastewater treatment plant using four parallel processes

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