Naegleria genus belongs to the flagellate free-living amoebae, which is ubiquitous in the environment (De Jonckheere, 2011). They have been found in various water resources (rivers, lakes and hot springs) and soil environments all around the world (De Jonckheere, 2011). Naegleria genus is classified in the Vahlkampfiidae family consisting of 47 species. There are three morphological forms to its life cycle: feeding trophozoite stage, transitional flagellate stage and dormant cyst stage (Siddiqui, Ali, Cope, & Khan, 2016). Naegleria fowleri is the only pathogen species of the genus Naegleria that is commonly known as brain-eating amoebae. It can cause a rare and devastating