Occurrence and Molecular Phylogeny of Honey Bee Viruses in Vespids

Yang, Sa; Gayral, Philippe; Zhao, Hongxia; Wu, Yaojun; Jiang, Xuejian; Wu, Yanyan; Bigot, Diane; Wang, Xinling; Yang, Dahe; Herniou, Elisabeth A.; Deng, Shuai; Li, Fēi; Diao, Qingyun; Darrouzet, Éric; Hou, Chunsheng

doi:10.3390/v12010006

Cited by 25 publications

(46 citation statements)

References 35 publications

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“…Moreover, consuming bees by carnivors may be another route of transmission. Indeed, several wasps have been detected as positive for many viruses, and active replication of DWV, AKI-complex and BQCV has been shown [ 45 , 68 , 69 , 70 , 71 ]. When infected, other horizontal routes of infection related to the social structure and life history traits of the wild bees (trophallaxis, larval food) may increase intra-specific transmission (see Yañez et al [ 50 ] for review).…”

Section: Introductionmentioning

confidence: 99%

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Dalmon

Diévart

Thomasson

et al. 2021

Insects

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Viruses are known to contribute to bee population decline. Possible spillover is suspected from the co-occurrence of viruses in wild bees and honey bees. In order to study the risk of virus transmission between wild and managed bee species sharing the same floral resource, we tried to maximize the possible cross-infections using Phacelia tanacetifolia, which is highly attractive to honey bees and a broad range of wild bee species. Virus prevalence was compared over two years in Southern France. A total of 1137 wild bees from 29 wild bee species (based on COI barcoding) and 920 honey bees (Apis mellifera) were checked for the seven most common honey bee RNA viruses. Halictid bees were the most abundant. Co-infections were frequent, and Sacbrood virus (SBV), Black queen cell virus (BQCV), Acute bee paralysis virus (ABPV) and Israeli acute paralysis virus (IAPV) were widespread in the hymenopteran pollinator community. Conversely, Deformed wing virus (DWV) was detected at low levels in wild bees, whereas it was highly prevalent in honey bees (78.3% of the samples). Both wild bee and honey bee virus isolates were sequenced to look for possible host-specificity or geographical structuring. ABPV phylogeny suggested a specific cluster for Eucera bees, while isolates of DWV from bumble bees (Bombus spp.) clustered together with honey bee isolates, suggesting a possible spillover.

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Section: Introductionmentioning

confidence: 99%

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Dalmon

Diévart

Thomasson

et al. 2021

Insects

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“…Concerning DWV-B, it was detected with a high alignment rate in all samples (Accession numbers: MT747986; MT747989; MT747988; MT747909; MT747987), demonstrating the high diffusion of the virus worldwide (Wilfert et al, 2016). In recent studies conducted on V. velutina, DWV-A was found in adult hornets sampled in France (Yang et al, 2020), while in another study performed on hornets from the same country, DWV-B was found in F I G U R E 2 2Coverage and position of consensus sequences aligned against the reference whole genome. Accession number of the reference genome is indicated on the box referred to each detected virus.…”

Section: Discussionmentioning

confidence: 85%

“…Moreover, in V. velutina sampled in South West of France the replicative form of Sacbrood virus (SBV), Moku virus and Israeli Acute Paralysis Virus (IAPV) were also detected (Garigliany et al, 2017;Mordecai et al, 2016;Yañez et al, 2012). Yang et al (2020) detected DWV and SBV in V. velutina sampled in France as well, while in such hornet species sampled in China, only IAPV was reported. Recently, both Kashmir bee Virus (KBV) and Black Queen Cell Virus (BQCV) were detected in V. velutina in Italy, although in an asymptomatic form (Mazzei et al, 2019).…”

Section: Introductionmentioning

confidence: 97%

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Next generation sequencing study on RNA viruses ofVespa velutinaandApis melliferasharing the same foraging area

Marzoli

Forzan

Bortolotti

et al. 2020

Transbound Emerg Dis

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The predator Asian hornet (Vespa velutina) represents one of the major threats to honeybee survival. Viral spillover from bee to wasp has been supposed in several studies, and this work aims to identify and study the virome of both insect species living simultaneously in the same foraging area. Transcriptomic analysis was performed on V. velutina and Apis mellifera samples, and replicative form of detected viruses was carried out by strand-specific RT-PCR. Overall, 6 and 9 different viral types were reported in V. velutina and A. mellifera, respectively, and five of these viruses were recorded in both hosts. Varroa destructor virus-1 and Cripavirus NB-1/2011/HUN (now classified as Triato-like virus) were the most represented viruses detected in both hosts, also in replicative form. In this investigation, Triato-like virus, as well as Aphis gossypii virus and Nora virus, was detected for the first time in honeybees.Concerning V. velutina, we report for the first time the recently detected honeybee La Jolla virus. A general high homology rate between genomes of shared viruses between V. velutina and A. mellifera suggests the efficient transmission of the virus from bee to wasp. In conclusion, our findings highlight the presence of several known and newly reported RNA viruses infecting A. mellifera and V. velutina. This confirms the environment role as an important source of infection and indicates the possibility of spillover from prey to predator.

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“…Newly emerged bees were obtained from brood frames taken from the experimental honey bee hives and kept in an incubator at 30 • C and a 60% relative humidity (RH) for approximately 12 h until use for injecting RNA transcripts. We confirmed the absence of the eight common viruses before the experiment was performed as previously described [44].…”

Section: Honey Bee Samplesmentioning

confidence: 85%

A Reverse Genetics System for the Israeli Acute Paralysis Virus and Chronic Bee Paralysis Virus

Yang

Zhao²,

Deng

et al. 2020

IJMS

Self Cite

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Honey bee viruses are associated with honey bee colony decline. Israeli acute paralysis virus (IAPV) is considered to have a strong impact on honey bee survival. Phylogenetic analysis of the viral genomes from several regions of the world showed that various IAPV lineages had substantial differences in virulence. Chronic bee paralysis virus (CBPV), another important honey bee virus, can induce two significantly different symptoms. However, the infection characteristics and pathogenesis of IAPV and CBPV have not been completely elucidated. Here, we constructed infectious clones of IAPV and CBPV using a universal vector to provide a basis for studying their replication and pathogenesis. Infectious IAPV and CBPV were rescued from molecular clones of IAPV and CBPV genomes, respectively, that induced typical paralysis symptoms. The replication levels and expression proteins of IAPV and CBPV in progeny virus production were confirmed by qPCR and Western blot. Our results will allow further dissection of the role of each gene in the context of viral infection while helping to study viral pathogenesis and develop antiviral drugs using reverse genetics systems.

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Occurrence and Molecular Phylogeny of Honey Bee Viruses in Vespids

Cited by 25 publications

References 35 publications

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource

Next generation sequencing study on RNA viruses ofVespa velutinaandApis melliferasharing the same foraging area

A Reverse Genetics System for the Israeli Acute Paralysis Virus and Chronic Bee Paralysis Virus

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