Next generation sequencing study on RNA viruses ofVespa velutinaandApis melliferasharing the same foraging area

Abstract

The predator Asian hornet (Vespa velutina) represents one of the major threats to honeybee survival. Viral spillover from bee to wasp has been supposed in several studies, and this work aims to identify and study the virome of both insect species living simultaneously in the same foraging area. Transcriptomic analysis was performed on V. velutina and Apis mellifera samples, and replicative form of detected viruses was carried out by strand-specific RT-PCR. Overall, 6 and 9 different viral types were reported in V. velutina and A. mellifera, respectively, and five of these viruses were recorded in both hosts. Varroa destructor virus-1 and Cripavirus NB-1/2011/HUN (now classified as Triato-like virus) were the most represented viruses detected in both hosts, also in replicative form. In this investigation, Triato-like virus, as well as Aphis gossypii virus and Nora virus, was detected for the first time in honeybees.Concerning V. velutina, we report for the first time the recently detected honeybee La Jolla virus. A general high homology rate between genomes of shared viruses between V. velutina and A. mellifera suggests the efficient transmission of the virus from bee to wasp. In conclusion, our findings highlight the presence of several known and newly reported RNA viruses infecting A. mellifera and V. velutina. This confirms the environment role as an important source of infection and indicates the possibility of spillover from prey to predator.

Evidence for multiple introductions of an invasive wild bee species currently under rapid range expansion in Europe

“…Bees are generally considered beneficial for the pollination services they provide, but they may have surprising negative impacts on local ecosystems where they do not naturally occur [1][2][3][4]. Negative impacts can take different forms, including through disrupting plant-pollinator networks, competition with native species for nest sites or other resources, pollinating invasive plants, the introduction of novel pathogens, and economic losses [5][6][7][8]. Among bees, those which nest in cavities opportunistically are disproportionately invasive, i.e.…”

Pathogens Spillover from Honey Bees to Other Arthropods

“…The following are available online at , Table S1: Bee pathogen spillover and prevalence identified in hymenopteran hosts, of which are reported condition, stage, geographical area and year, Table S2: Bee pathogen spillover and prevalence identified in arthropod hosts, of which are reported condition, stage, geographical are and year [ 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 ].…”

Metagenomic Analysis of Togaviridae in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes from Chikungunya and Ross River Viruses

“…Compared with the traditional method, the efficiency of metagenomic sequencing by Illumina sequencing combined with bioinformatics analysis is higher, and in this way, many viruses from different species have been identified in recent years (Zhang et al, 2018;Marzoli et al, 2021;Thannesberger et al, 2021). In addition, the component of mosquito virome and the high abundance of viruses in mosquitoes can be revealed using metagenomic sequencing (Metsky et al, 2019;Ramıŕez et al, 2020).…”