Occurrence and genotypes of Giardia isolated from lambs in Spain

Gómez-Muñoz, María Teresa; Navarro, Cristina; Garijo-Toledo, María Magdalena; Dea-Ayuela, M. Auxiliadora; Fernández-Barredo, Salceda; Pérez‐Gracia, María Teresa; Domínguez-Márquez, M. Victoria; Borrás, Rafael

doi:10.1016/j.parint.2009.06.005

Cited by 24 publications

(20 citation statements)

References 19 publications

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“…The lambs and ewes were predominantly infected with G. duodenalis genotype E, which is not infectious for humans. This genotype is the most prevalent genotype in sheep worldwide (Santín et al 2007;Geurden et al 2008;Wang et al 2010;Robertson et al 2010), although the zoonotic genotypes A (Giangaspero et al 2005;Ryan et al 2005;Gómez-Muñoz et al 2009) and B (Aloisio et al 2006) have also been detected in sheep in other studies.…”

mentioning

confidence: 76%

“…The prevalence of G. duodenalis in sheep ranged from 1.5% to 43% in surveys from North America and Europe (Giangaspero et al 2005;Santín et al 2007;Geurden et al 2008;Gómez-Muñoz et al 2009). Cryptosporidium-infected sheep have been detected worldwide (Wang et al 2010;Robertson et al 2010;Amer et al 2010), and specifically in Belgium (Geurden et al 2008), the United Kingdom (Elwin and Chalmers 2008), the USA (Santín et al 2007), Australia (Ryan et al 2005;Yang et al 2009).…”

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confidence: 99%

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High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

Silva¹,

Lopes

Bresciani

et al. 2014

Acta Parasitologica

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In this paper we report the occurrence of zoonotic species of Cryptosporidium (C. ubiquitum and C. parvum subtype IIaA15G2R1) and the host-specific Giardia duodenalis genotype E in a sheep farm from Brazil. Fecal specimens were collected from 100 animals and screened for the presence of G. duodenalis and Cryptosporidium spp. by enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequence analysis of selected samples was used to confirm the species and to characterize the isolates. Of 100 specimens, 34 were positive for Giardia (34%) and 25 were positive for Cryptosporidium (25%). The prevalence of Giardia and Cryptosporidium was higher in lambs than in ewes. Co-infection was observed in 14% of animals. All G. duodenalis isolates belonged to genotype E. The genetic characterization performed in 22 Cryptosporidium-positive samples revealed the presence of zoonotic C. ubiquitum and C. parvum in 76% (19/25) and 12% (3/25) of infected animals, respectively. The presence of zoonotic Cryptosporidium subtypes in lambs was of epidemiological and public health relevance.

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confidence: 76%

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confidence: 99%

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

Silva¹,

Lopes

Bresciani

et al. 2014

Acta Parasitologica

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“…Similarly, isolates C1, C3, C4, C14 and C16 showed 100% identity to the published sequences from genotype E also obtained from goats in Spain (Ruiz et al 2008). Moreover, a BLAST analysis showed 100% similarity between these isolates and genotype E samples obtained from sheep in Mexico (Di Giovanni et al 2006) and in Spain (Gomez-Munoz et al 2009). …”

Section: Resultsmentioning

confidence: 90%

“…The tpi gene target was more variable, which was also reported by Gomez-Munoz et al (2012) and Fava et al (2013). However, because SNPs could represent sequencing artefacts, sequence heterozygosis, or mixed infections, these findings should be carefully evaluated and more studies are needed to determine the real value of these data.…”

Section: Discussionmentioning

confidence: 91%

“…A large number of Giardia duodenalis genotyping studies in ruminants report a higher occurrence of genotype E in these animals, with genotypes A and B being less frequent (Castro-Hermida et al 2007;Geurden et al 2008;Gomez-Munoz et al 2009;Zhang et al 2012). However, Geurden et al (2008) and Berrilli et al (2012) reported that zoonotic genotype A infected goats in Belgium and Côte d´Ivoire, respectively, and Lim et al (2013) reported that genotypes A and B infected goats in Malaysia.…”

Section: Discussionmentioning

confidence: 99%

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First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

Sudré¹,

Leles²,

Lima³

et al. 2014

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to perform the first molecular genotyping of Giardia duodenalis from goats in Brazil, in order to assess the risk for zoonotic transmission. Samples were collected from two dairy goat farms (Saanen breed) located in the city of Niteroi in the state of Rio de Janeiro, Brazil. Goat faecal samples (n = 58) were collected directly from the rectums of all animals up to one year of age and were subjected to centrifuge-flotation in sugar saturate solution. Nested-PCR and sequencing using β-giardin and tpi gene targets was performed on positive samples. Seventeen out of fifty-eight (29.31%) faecal samples were positive for Giardia duodenalis cysts, all belonging to the same farm. Only eight isolates were successfully sequenced (eight samples for β-giardin and four samples for tpi), all belonging to genotype E. Two types of sequences were identified for each locus within isolates from the current study, which exhibited sequence heterogeneity with variable numbers of single nucleotide polymorphisms. The present study contributes to a better understanding of the molecular epidemiology of this parasite.

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Giardiasis: A Zoonotic Infection or Not?

Cacciò

2014

Zoonoses - Infections Affecting Humans and Animals

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Occurrence and genotypes of Giardia isolated from lambs in Spain

Cited by 24 publications

References 19 publications

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

First molecular characterisation of Giardia duodenalis infection in dairy goats in Brazil

Giardiasis: A Zoonotic Infection or Not?

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