Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia

Wardhana, April Hari; Sawitri, Dyah Haryuningtyas; Ekawasti, Fitrine; Martindah, Eny; Apritadewi, Dias; Shibahara, Tomoyuki; Kusumoto, Masahiro; Tokoro, Masaharu; Sasai, Kazumi; Matsubayashi, Makoto

doi:10.1007/s00436-020-06806-0

Cited by 8 publications

(22 citation statements)

References 27 publications

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“…pada babi di Indonesia lebih banyak dilakukan berdasarkan uji parasitologis (morfologi) (Suryawan et al, 2014;Yuliari et al, 2013), tetapi identifikasi secara molekuler masih terbatas. Beberapa peneliti yang telah melaporkan penelitian molekuler Entamoeba di antaranya Wardhana et al, (2020) yang melaporkan prevalensi E. suis di Tangerang, Jawa Barat sebesar 81,1%. Agustina et al, (2016) juga melaporkan prevalensi Entamoeba spp.…”

Section: Pendahuluanunclassified

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Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya

et al. 2021

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Penelitian ini bertujuan untuk mendeteksi E. suis pada babi yang dipotong di Rumah Potong Hewan (RPH) Pegirian, Surabaya. Sebanyak 63 sampel feses babi dikoleksi dari RPH Pegirian Surabaya. Deteksi morfologis Entamoeba spp. dilakukan dengan metode apung (analisis parasitologi). Selain itu, penentuan spesies E. suis dilakukan dengan PCR menggunakan primer Esuis764 (5’-ATC AAA TCA ATT AGG CAT AAC TA-3’) dan Esuis765 (5’-AAT TAA AAC CTT ACG GCT TTA AA-3’) dan prodak PCR yang diharapkan adalah 320 bp. DNA dari sampel positif diekstraksi dengan DNAzol. PCR dilakukan pada beberapa sampel positif yang dipilih secara acak dari analisis parasitologi. Hasil penelitian menunjukkan 57,14% (36/63) sampel secara morfologis positif Entamoeba sp. Hasil penentuan spesies berdasarkan analisis molekuler menemukan bahwa protozoa yang menginveksi babi di Rumah Potong Hewan Pegirian, Surabaya adalah E. suis.

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Section: Pendahuluanunclassified

“…Salah satu studi terbaru tentang Entamoeba spp. pada babi secara molekuler dilaporkan oleh Wardhana et al (2020). Kasus infeksi E. suis pada peternakan babi di Tangerang, Jawa Barat dilaporkan mencapai 81,1%.…”

Section: Hasil Dan Pembahasanunclassified

Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya

et al. 2021

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“…Of note, there was no evidence of Entamoeba suis-specific DNA in the samples. E. suis was observed in 81.1% samples from pigs sampled in West Java, Indonesia [66], while Ji et al observed a considerable lower positive rate (13.0%) for E. suis in pigs sampled in south-eastern China [65]. To our knowledge, E. suis is yet to be reported and confirmed at DNA-level in Europe.…”

Section: Discussionmentioning

confidence: 77%

“…polecki was divided into four specific subtypes by Verweij et al in 2001 [64], all of which have been isolated from human stool, but which appear to differ in terms of host reservoir specificity. Of these four subtypes, ST1 and ST3 appear to be common in pigs [16,63,65,66]. Meanwhile ST4 has only been found in humans so far, whereas ST2 has been found in non-human primates as well.…”

Section: Discussionmentioning

confidence: 99%

Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR

et al. 2021

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Several parasite species are shared between humans and pigs. We explored the application of next-generation sequencing-based metabarcoding supplemented with real-time PCR to fecal DNAs from 259 samples from 116 pigs in Denmark to detect and differentiate single-celled intestinal parasites of zoonotic relevance. Enterocytozoon bieneusi, Balantioides coli, and Giardia duodenalis were observed in 34/37 (92%), 148/259 (57%), and 86/259 (33%) samples, respectively. Entamoeba polecki ST1, E. polecki ST3, and Entamoeba hartmanni were detected in 104/259 (40%), 161/259 (62%), and 8/259 (3%) samples, respectively. Metabarcoding and real-time PCR detected Cryptosporidium in 90/259 (35%) and 239/259 (92%) of the samples, respectively, with Cryptosporidium suis and Cryptosporidium scrofarum observed in nearly equal proportions. Blastocystis subtypes 1, 3, 5, and 15 were found in 72 (28%), 6 (2%), 176 (68%), and 36 (14%) of 259 samples, respectively. Iodamoeba was identified in 1/259 samples (<1%), while none of 37 tested samples was positive for Dientamoeba fragilis. Our results illustrate how metabarcoding exemplifies a ‘one-fits-many’ approach to detecting intestinal single-celled parasites in feces supplemented with real-time PCR for selected parasites. Using metabarcoding with pathogen-specific assays may help detect emerging and previously underdetected pathogens and further elucidate the role of micro-eukaryotic parasites in human and animal health and disease.

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“…with other studies in healthy pigs, it was higher than in Korea (3.7%) [ 32 ], India (50%) [ 33 ], Greece (8.3%) [ 34 ], Ireland (8% for E. suis and 17% for E. polecki ) [ 29 ], Cambodia (31.6%) [ 35 ], Germany (52%) [ 36 ] and the UK (0%) [ 37 ]. It also was higher than that in Vietnam (91.7%) [ 37 ], Anhui Province (45.8%) [ 14 ] and Fujian Province (55.4%) [ 25 ], but lower than that in Tibet (37.9%) [ 38 ] and Indonesia (81.1% for E. suis and 35.7% for E. polecki ) [ 39 ]. Large differences in the infection of Entamoeba spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Entamoeba spp. in Pigs with Diarrhea in Southern China

Wang

Zou

et al. 2022

Animals

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Entamoeba spp. is a common zoonotic intestinal protozoan that can parasitize most vertebrates, including humans and pigs, causing severe intestinal diseases and posing a serious threat to public health. However, the available data on Entamoeba spp. infection in pigs are relatively limited in China. To characterize the infection of Entamoeba spp. within pigs in southern China, 1254 fecal samples of diarrheic pigs were collected from 37 intensive pig farms in Hunan, Jiangxi and Fujian provinces and the infection of Entamoeba spp. was investigated based on the small subunit rRNA (SSU rRNA) gene. The overall infection rate of Entamoeba spp. was 58.4% (732/1254), including 38.4% (118/307) in suckling piglets, 51.2% (153/299) in weaned piglets, 57.9% (55/95) in fattening pigs and 73.4% (406/553) in sows, respectively. Moreover, age and the sampling cities in Jiangxi and Fujian provinces were found to be the key factors influencing the infection of Entamoeba spp. (p < 0.05). Two subtypes (ST1 and ST3) with a zoonotic potential of Entamoeba polecki and Entamoeba suis were detected in all age groups of pigs and all sampling areas, with the predominant species and predominant subtype being E. polecki (91.3%, 668/732) and E. polecki ST1 (573/668), respectively, and E. polecki ST1 + E. polecki ST3 (78.6%, 239/304) being the most frequently detected form of mixed infection. Severe Entamoeba spp. infection and zoonotic subtypes were found in this study, exposing a large public health problem in the study area, and strategies need to be implemented to eliminate the risk in the future.

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Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia

Cited by 8 publications

References 27 publications

Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya

Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya

Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR

Molecular Characterization of Entamoeba spp. in Pigs with Diarrhea in Southern China

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