Occurrence and ecotoxicity of cytostatic drugs 5-fluorouracil and methotrexate in the freshwater unionid Elliptio complanata

Kleinert, Christine; Poirier-Larabie, Sylvie; Gagnon, Christian; André, C.; Gagné, François

doi:10.1016/j.cbpc.2021.109027

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The main objective of the MTX exposure experiment with mussels was to investigate short-term uptake and provide insights on the chronic exposure potential as a part of a broader investigation of ecotoxicological impacts. The toxicological effects of MTX were demonstrated by the increase in dehydrofolate reductase (DHFR) activity in the gonad and correlated with GST activity (Kleinert et al 2021). Given that MTX is known inhibitor of DHFR activity, the observed induction represents an adaptive response of these mussels to maintain folate for purine (DNA) biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of uptake of the cytostatic methotrexate in Elliptio complanata mussels by LC–MS/MS

Larabie

Jutras

Leclair

et al. 2022

Environ Sci Pollut Res

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Aquatic organisms are continuously exposed to emerging contaminants coming from urban effluents of wastewater treatment plants. The contamination of surface water by those effluents poses a number of environmental risks, and pharmaceuticals are part of this class of effluent contaminants. Various classes of pharmaceuticals are not treated by wastewater treatment plants and anticancer drugs are part of them. The chemotherapy drug methotrexate (MTX) is an emerging contaminant and its growing use with the increase in cancer cases worldwide raises potential risk to aquatic organisms exposed to effluent discharges. However, chemical analyses in exposed freshwater aquatic organisms for ecotoxicological studies are rarely available and no studies have been done yet to accompany ecotoxicological data of exposed filter-feeding organisms. The purpose of this study was to develop a specific and sensitive analytical LC–MS/MS method for the quantification of methotrexate uptake in mussels exposed at different concentrations of the drug. A solid/liquid extraction followed by solid phase extraction (SPE) using an MCX phase purification scheme was optimized. The optimal recovery of 65% and matrix effect of 38% allowed to achieve a limit of quantification of 0.25 ng g−1, with an accuracy of 99–106%, a precision of no more than 3% RSD, and linearity ranging from 0.25 to 25 ng g−1. This methodology was tested with mussels exposed for 96 h at different concentrations (4 to 100 µg L−1) of MTX. The data revealed tissue uptake at concentrations ranging from 0 to 2.53 ng g−1. This suggests that this drug has low uptake potential and this methodology could be used to examine tissue levels of this drug in organisms continuously exposed to urban pollution.

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Section: Discussionmentioning

confidence: 99%

Evaluation of uptake of the cytostatic methotrexate in Elliptio complanata mussels by LC–MS/MS

Larabie

Jutras

Leclair

et al. 2022

Environ Sci Pollut Res

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“…Anticancer drugs or cytostatic pharmaceuticals, as dangerous compounds, cause irreparable damage to the environment because their effective removal has not been possible from wastewater; they are present in high concentrations (up to 2.12 × 10 –4 mg L −1 ) in the aqueous media (Jureczko and Kalka 2020 ). Cytostatic contaminants, due to their mode of action on DNA damage by disturbing the repair activity and inhibiting the de novo synthesis, can endanger the organisms’ health (Kleinert et al 2021 ).…”

Section: Pharmaceuticalsmentioning

confidence: 99%

“…Exposure to environmental concentrations of 5-fluorouracil and cyclophosphamide pollutants (0.2–123 μg L −1 ) for Lithobates catesbeianus (tadpoles) can generate morphological changes and mutagenic effects in the gastrointestinal tract via formation of binuclei and micronuclei as well as melanocytes development (Araújo et al 2019 ). The toxicity results for 5-fluorouracil and methotrexate to endemic Elliptio complanata (freshwater mussels) at the highest concentration (100 μg L −1 ) for 96 h indicate that methotrexate and 5-fluorouracil with a larger spectrum of effects can increase the dihydrofolate reductase and can decrease the DNA repair activity and lipid peroxidation (Kleinert et al 2021 ).…”

Section: Pharmaceuticalsmentioning

confidence: 99%

Remediation of pharmaceuticals from contaminated water by molecularly imprinted polymers: a review

Zare‬

Fallah

et al. 2022

Environ Chem Lett

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The release of pharmaceuticals into the environment induces adverse effects on the metabolism of humans and other living species, calling for advanced remediation methods. Conventional removal methods are often non-selective and cause secondary contamination. These issues may be partly solved by the use of recently-developped adsorbents such as molecularly imprinted polymers. Here we review the synthesis and application of molecularly imprinted polymers for removing pharmaceuticals in water. Molecularly imprinted polymers are synthesized via several multiple-step polymerization methods. Molecularly imprinted polymers are potent adsorbents at the laboratory scale, yet their efficiency is limited by template leakage and polymer quality. Adsorption performance of multi-templated molecularly imprinted polymers depends on the design of wastewater treatment plants, pharmaceutical consumption patterns and the population serviced by these wastewater treatment plants.

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Assessing the effects of the cytostatic drug 5-Fluorouracil alone and in a mixture of emerging contaminants on the mussel Mytilus galloprovincialis

2022

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Occurrence and ecotoxicity of cytostatic drugs 5-fluorouracil and methotrexate in the freshwater unionid Elliptio complanata

Cited by 4 publications

References 25 publications

Evaluation of uptake of the cytostatic methotrexate in Elliptio complanata mussels by LC–MS/MS

Evaluation of uptake of the cytostatic methotrexate in Elliptio complanata mussels by LC–MS/MS

Remediation of pharmaceuticals from contaminated water by molecularly imprinted polymers: a review

Assessing the effects of the cytostatic drug 5-Fluorouracil alone and in a mixture of emerging contaminants on the mussel Mytilus galloprovincialis

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