Occurrence and control of
            <i>Naegleria fowleri</i>
            in drinking water wells.

Gerba, Charles P.; Blair, Barbara; Sarkar, Payal; Bright, Kelly R.; MacLean, R. Craig; Marciano-Cabral, Francine

doi:10.1079/9781845933913.0238

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent findings have reported Ct values of 6 mg Cl 2 min l −1 for N. fowleri trophozoites and 31 mg Cl 2 min l −1 for cysts, leading to 3‐log inactivation of the pathogenic amoeba with chlorine (Gerba et al . ; Sarkar and Gerba ). Operational control and management of Naegleria based on the application of contact times greater than 30 mg Cl 2 min l −1 and maintenance of a free chlorine residual of 0·2 mg l −1 in the end section of water distribution systems was tried in Australia and has proved to be effective (Trolio et al .…”

Section: Discussionmentioning

confidence: 96%

Biocidal efficacy of monochloramine against planktonic and biofilm-associated Naegleria fowleri cells

Goudot

Herbelin²,

Mathieu

et al. 2014

J Appl Microbiol

View full text Add to dashboard Cite

Aims: Free-living amoebae (FLA) in aqueous systems are a problem for water network managers and health authorities because some are pathogenic, such as Naegleria fowleri, and they have also been reported to operate as reservoirs and vectors of several pathogenic bacteria. Therefore, to better control the occurrence of such amoebae, we evaluate the efficacy of monochloramine against planktonic forms (trophozoites and cysts) and also biofilm-associated cells of N. fowleri as FLA are often associated with biofilms. Methods and Results: From a freshwater biofilm growing in a pilot reactor and inoculated with N. fowleri, we obtained Ct values ranging from 4 to 17 mg Cl 2 min l À1 at 25°C and pH 8Á2 on both planktonic and biofilm associated cells. In addition, the inactivation pattern of biofilm associated was intermediate between those of trophozo€ ıtes and cysts. Conclusions:The monochloramine efficiency varies with the life stage of N. fowleri (trophozo€ ıte, cyst, and biofilm-associated). The sensitivity to disinfectant of amoeba, that is, trophozo€ ıtes and cysts, in the biofilm life stage is as high as that of their planktonic cyst form. Significance and Impact of the Study: This study gives Ct values for cysts and biofilm-associated N. fowleri. This may impact on water treatment strategies against amoebae and should be considered when controlling N. fowleri in manmade water systems such as cooling towers or hot water systems.

show abstract

Section: Discussionmentioning

confidence: 96%