Occurrence and characterization of Campylobacter spp.isolates in dogs, cats and children

Abstract

RESUMO.-[Ocorrência e caracterização de isolados de Campylobacter spp. em cães, gatos e crianças.] Com o objetivo de melhorar o entendimento das infecções porCampylobacter spp. em cães, gatos e crianças no Brasil, foram avaliadas 160 amostras fecais de crianças e 120 swabs retais de pets (103 cães e 17 gatos). Do total das amostras das crianças, 6,87% foram positivas para Campylobacter spp. e em cães e gatos a positividade foi de 18,3%. Das 33 amostras positivas para Campylobacter spp., 57,6% foram identificadas como C. jejuni e 33,4% foram identificadas como C. coli. Mais de 50% das amostras isoladas de pets foram resistentes a ceftiofur, sulphazotrim, norfloxacina e tetraciclina. Em crianças, a maioria das amostras foi resistente a amoxilina, cefazolina, ceftiofur, eritromicina e norfloxacina. De 19 isolados de C. jejuni, 11 isolados de crianças e cinco (5) de cães tinham dois (2) dos quatro (4) genes de virulência flaA, pldA, cadF or ciaB. Associação positiva entre a presença de Campylobacter spp. e diarreia em cães e gatos foi observada em animais desverminados e com hemograma sugestivo de infecção bacteriana. Também houve associação positiva entre a presença dos genes de virulên-cia e a ocorrência de diarreia, e entre o uso de antibióticos e a positividade para Campylobacter spp. em suabes fecais de pets. To improve the understanding of implications of Campylobacter spp. infections in pets and children of different environments were analysed 160 faecal samples from children and 120 from pets (103 dogs and 17 cats). Campylobacter spp. were detected in 6.87% of the children and in 18.3% of the dogs and cats. From 33 stool samples positive for Campylobacter spp., 57.6% were identified as C. jejuni, and 33.4% were identified as C. coli. More than 50% of the isolates in pets were resistant to ceftiofur, sulphazotrim, norfloxacin and tetracycline. In humans, most of the isolates were resistant to amoxicillin, cefazolin, ceftiofur, erythromycin and norfloxacin. From 19 isolates of C. jejuni, 11 isolates from children and 5 from dogs contained two to four of the virulence genes flaA, pldA, cadF or ciaB. We found an association between the presence of virulence genes and diarrhoea. Furthermore, an association was observed between the presence of Campylobacter spp. and diarrhoea in dewormed pets with blood picture suggestive of bacterial infection, and the therapeutic use of antibiotics was associated with more positive detection of Campylobacter spp. in the faeces of pets. Our data indicate that virulent strains of Campylobacter spp. can be risk factor to diarrhoea in animals, and that high resistance to antimicrobial agents is common in pets. de Campylobacter spp. são fatores de risco para diarreia em cães e a resistência antimicrobiana é comum em isolados de cães.

Isolation, Molecular Detection, and Risk Factors of Campylobacter Infection From Companion Dogs

“…The other studies reported Campylobacter infection rates of 4.81-51.1%. 5,[22][23][24][25][26][27] The variation in the rate of infection may rely on the sample size, age, diet, and gastrointestinal status (diarrheal or healthy) of the studied dogs, different diagnosis methods, and geographical location, as well as the season of sampling. The most prevalent species of Campylobacter among the dogs were C. coli (38.89%) and C. jejuni (33.33%).…”

The prevalence of virulence genes and multidrug resistance in thermophilic Campylobacter spp. isolated from dogs

“…Our study showed a high percentage of the cadF , flaA genes and iam sequence in both groups of dogs. Rodrigues et al [ 33 ] observed a high incidence of the cadF and flaA genes in dogs and noted that these genes may act as reporters in research on the mechanisms of C. jejuni pathogenicity.…”

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

“…Moreover, CJ multidrug resistance to different antibiotics used in poultry and humans has been currently noticed. It was described that children and pets with CJ infections were resistant to ceftiofur, sulphazotrim, norfloxacin, tetracycline, amoxicillin, ciprofloxacin, cefazolin, and erythromycin antibiotics ( Rodrigues et al., 2015 ). Furthermore, another work showed that CJ isolated from chicken carcasses were highly resistant to norfloxacin, erythromycin, and amoxicillin, the most used drugs in traditional antibiotic therapy ( Fonseca et al., 2016 ).…”