Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil

Alves, Daniella de Sousa Mendes Moreira; Moraes, Aline Silva; Nitz, Nadjar; Oliveira, Márcia Rozenfeld Gomes de; Hecht, Markus; Gurgel-Gonçalves, Rodrigo; Cuba, César Augusto Cuba

doi:10.1016/j.exppara.2012.04.011

Cited by 33 publications

(21 citation statements)

References 37 publications

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“…Based on rRNA gene sequences, the genus Acanthamoeba is divided into 20 different genotypes to date (T1-T20). Each genotype exhibits 5% or more sequence divergence [9,20,21].…”

Section: Discussionmentioning

confidence: 99%

First report of Acanthamoeba genotype T8 human keratitis

Orosz

Szentmáry

Kiss

et al. 2018

AMicr

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Acanthamoeba has a worldwide distribution in the environment and it is capable of causing a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). Nowadays, the cases of AK have surged all over the world along with its disease burden due to increasing use of contact lenses used not only for optical correction but also for cosmetic purposes. In our present work, epithelial abrasion of a 27-year-old female soft contact lens wearer with keratitis was examined. Genotype identification was carried out with a real-time fluorescence resonance energy transfer polymerase chain reaction (PCR) assay based on sequence analysis of the 18S rRNA gene. Genotyping allowed the identification of a T8 group isolate. The analysis confirmed the importance of a complete diagnostic protocol, including a PCR assay, for the clinical diagnosis of AK from human samples. Acanthamoeba T8 should be considered as potential causative organism in keratitis in human.

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“…Based on rRNA gene sequences, the genus Acanthamoeba is divided into 20 different genotypes to date (T1-T20). Each genotype exhibits 5% or more sequence divergence [9,20,21].…”

Section: Discussionmentioning

confidence: 99%

First report of Acanthamoeba genotype T8 human keratitis

Orosz

Szentmáry

Kiss

et al. 2018

AMicr

View full text Add to dashboard Cite

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“…The culture-confirmed detection method is slow (96 h) and can In conclusion, AK has emerged as a clinical problem only during the last 45 years and has been one of the most difficult corneal diseases. The lack of appropriate hand washing and the use of non-sterile home-made saline solutions for CLs are also linked to the infection [15,16]. Recently, treatment has become more effective and diagnosis is often made earlier than in the past, due to increased familiarity with the condition.…”

Section: Discussionmentioning

confidence: 99%

Laboratory diagnosis of Acanthamoeba keratitis in Hungary

Orosz

Farkas

Kucsera

2016

Acta Microbiologica et Immunologica Hungarica

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Acanthamoeba species are free-living amebae that can be found in almost every range of environments. Within this genus, numerous species are recognized as human pathogens, potentially causing Acanthamoeba keratitis (AK). AK is a corneal disease that is predominantly associated with contact lens use, the epidemiology of which is related to the specific genotype of Acanthamoeba. This study reports seven (7/16; 43.75%) positive cases. Detection of Acanthamoeba in corneal scrapings is based on cultivation and polymerase chain reaction (PCR) combined with the molecular taxonomic identification method. By PCR, seven samples were positive; cultivation was successful for five samples, probably because of the low quantity of samples. Genotype identification was carried out with a real-time fluorescence resonance energy transfer PCR assay based on sequence analysis of the 18S rRNA gene, and sensitivity and specificity were evaluated in comparison with traditional parasitological techniques. All seven detected Acanthamoeba strains belonged to the T4 genotype, the main AK-related genotype worldwide. These results confirmed the importance of a complete diagnostic protocol, including a PCR assay, for the clinical diagnosis of AK from human samples. Genotyping allowed the identification of all isolates in the T4 group, thus demonstrating the prevalence of this genotype in Hungary.

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“…was 42.4% in water samples from Zabol, Iran (10) and 84.6% and 58.6% in water and soil samples from Pakistan, respectively (11). Also, in Brazil, 55% of stagnant water samples were infected with Acanthamoeba (12).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran

Golestani

Rasti

Hooshyar

et al. 2018

Jundishapur J Microbiol

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Background: Acanthamoeba is one of the most common opportunistic free-living amoebae, with ubiquitous presence in various environmental sources. Pathogenic strains of Acanthamoeba are the causative agents of amoebic keratitis and granulomatous amoebic encephalitis. Objectives: The aim of the present study was to identify Acanthamoeba genotypes in soil, hospital dust, and stagnant water samples from Kashan, Central Iran. Methods: In this cross sectional study, a total of 122 samples from soil (n, 32), hospital dust (n, 40), and stagnant water (n, 50) were collected and examined for the presence of free-living amoebae and Acanthamoeba species. All the samples were cultured onto non-nutrient agar plates for detection of free-living amoebae. Acanthamoeba species was identified by polymerase chain reaction (PCR) assay, using specific primers. A total of 29 Acanthamoeba isolates were sequenced, and different genotypes were detected via sequence analysis. Results:The results showed that 82.8% (101/122) of samples were positive for free-living amoebae. The PCR assay revealed that 62.5%, 52.5%, and 50% of soil, hospital dust, and stagnant water samples were positive for Acanthamoeba species, respectively. Moreover, T4, T5, T2, T7, and T11 genotypes were identified. The most common genotype was T4 (76%), isolated from stagnant water. Conclusions: Acanthamoeba is a prevalent species in the soil, hospital dust, and stagnant water of Kashan. As this protozoon can cause severe infections, health education and improvement of sanitation services are recommended for prevention of infection.

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Occurrence and characterization of Acanthamoeba similar to genotypes T4, T5, and T2/T6 isolated from environmental sources in Brasília, Federal District, Brazil

Cited by 33 publications

References 37 publications

First report of Acanthamoeba genotype T8 human keratitis

First report of Acanthamoeba genotype T8 human keratitis

Laboratory diagnosis of Acanthamoeba keratitis in Hungary

Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran

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