Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk

Geser, Nadine; Stephan, Roger; Hächler, Herbert

doi:10.1186/1746-6148-8-21

Cited by 300 publications

(281 citation statements)

References 39 publications

(49 reference statements)

Supporting

Mentioning

227

Contrasting

Unclassified

Order By: Relevance

“…This is at variance with the results of studies by Hiroi et al and Geser et al, in which layers are recognized as the major reservoir of ESBL-producing E. coli [14,15].…”

Section: Discussioncontrasting

confidence: 91%

Antimicrobial Resistance in ESBL-Producing Escherichia coli Isolated from Layer and Pig Farms in Thailand

Nuangmek

Rojanasthien²,

Chotinun³

et al. 2018

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Study of drug resistance of commensal bacteria in both humans and animals can determine the scale of the drug resistance problem. Usage of antimicrobials to treat infections in humans and animals has generated extensive antimicrobial pressure not only on targeted pathogens but also on commensal bacteria. Commensal Escherichia coli appears to be the major reservoir for resistant genes implicated in the transmission of genetic traits from one bacterium to another. Antimicrobial resistance in Enterobacteriaceae has increased dramatically worldwide in the last decade. An increasing number of community-onset extended-spectrum beta-lactamase (ESBL)-producing bacterial infections, especially those caused by ESBL-producing E. coli, have been reported in many countries, including Thailand. Moreover, ESBL-producing E. coli have been widely detected in food-producing animals and the environment. The increased use of ESBLs in food animals is a serious public health problem. The objective of the study was to determine the prevalence and antimicrobial resistance pattern of ESBL-producing E. coli isolated from pigs, layers, farm workers and stagnant water, in order to increase awareness about antimicrobial usage on farms and to minimize the expansion of the antimicrobial resistance phenomenon in farm settings. Materials, Methods & Results:A total of 588 samples were collected from 107 pig farms and 89 layer farms in Chiang Mai-Lamphun and Chon Buri provinces during May 2015-April 2016. Double-disk diffusion method according to EUCAST (European Committee on Antimicrobial Susceptibility Testing) guidelines was used for detection. The results demonstrated that 36.7% (216/588) of samples were ESBL-producing E. coli-positive, including rectal swabs 74.8% (80/107), pig farm worker stool swabs 57.0% (61/107), stagnant water on pig farms 21.5% (23/107), healthy layer rectal swabs 6.7% (6/89) and layer farm worker stool swabs 51.7% (46/89). Most of the isolates were resistant against ampicillin (99.5%), followed by erythromycin (98.6%) and ceftriaxone (96.3%). All of them were classified as multidrug-resistant strains. Moreover, AMP-CRO-E-TE-C-SXT-CN was the most frequent phenotype pattern detected in animals, humans and the environment, followed by AMP-CRO-E-TE-C-SXT-NA-CN. Discussion: The present study offers clear evidence that the prevalence of ESBL-producing E. coli in healthy pigs is higher than in layers. One possible explanation is that a large amount and variety of antimicrobials are used on pig farms, resulting in a common and significant source of drug-resistant ESBL-producing E. coli. The lower incidence of ESBL-producing E. coli in samples from a pig farm environment than in samples of animal origin indicate that pigs are a reservoir of a reservoir for resistant bacteria and a source of environmental contamination. Antimicrobial resistance patterns of ESBLproducing E. coli detected in all sample types and study locations were quite similar. In almost all ESBL-producing E. coli isolates, resistance was s...

show abstract

“…This is at variance with the results of studies by Hiroi et al and Geser et al, in which layers are recognized as the major reservoir of ESBL-producing E. coli [14,15].…”

Section: Discussioncontrasting

confidence: 91%

Antimicrobial Resistance in ESBL-Producing Escherichia coli Isolated from Layer and Pig Farms in Thailand

Nuangmek

Rojanasthien²,

Chotinun³

et al. 2018

Acta Scientiae. Vet.

View full text Add to dashboard Cite

show abstract

“…β-lactam antibiotics resistance have low toxicity, a factor that has resulted in overuse of these drugs within animal husbandry and the medical fraternity (Moyane et al, 2013). Few studies have noted Enterobacteriaceae resistance to β-lactam antibiotic in milk samples, however, a study by (Geser et al, 2012) reported resistance in E. coli from milk provinces. This may be as a result of the E. coli from different provinces in SA being exposed to similar antibiotics despite the geographical difference.…”

Section: Figmentioning

confidence: 99%

Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk

Ntuli

Njage

Buys

2016

Journal of Dairy Science

View full text Add to dashboard Cite

INTERPRETIVE SUMMARYCharacterization of E. coli and other Enterobacteriaceae in producer-distributor bulk milk, Ntuli, The direct sale of milk from producer-distributor (PD) to consumers is a common practice in many countries. We characterized E. coli and other Enterobacteriaceae in PD bulk milk sold at retail points in South Africa (SA). The study highlighted that there is a potential risk of transferring drug resistant pathogenic E. coli to consumers through the food chain.Information obtained from the study will be used to model the risk of pathogenic E. coli in PD milk and the proposal of most potentially effective mitigation efforts towards enhancing food security. ABSTRACTThe current study was undertaken to characterize Escherichia coli and other Enterobacteriaceae in raw and pasteurized producer-distributor bulk milk (PDBM). A total of 258 samples were collected from purchase points in 8 provinces in South Africa. The samples were tested for 1 antibiotic residues, phosphatase, total aerobic bacteria, coliforms and E. coli counts. Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used for identification of isolates. E. coli isolates were characterized for virulence factors, antimicrobial resistance, serotypes and presumptive E. coli O157:H7. Antibiotic residues and alkaline phosphatase were detected in 2 % (n=258) and 21 % (n=104) of the samples respectively. A total of 729 isolates belonging to 21 genera and 59 species were identified. E. coli, Enterobacter cloacae, Klebsiella oxytoca and Raoultella ornithinolytica were the most abundant species. Spoilage Enterobacteriaceae species exceeded 50 % of the total isolates. E. coli was detected and isolated from 36 % of the milk samples. Thirty one E. coli isolates harbored virulence genes stx1/stx2 and 38 % (n=121) were presumptive O157:H7. Prevalence of samples with presumptive shigatoxin producing E. coli (STEC) was 10 %. Antimicrobial resistant E. coli isolates were detected in 70 % of the milk samples with 36 % of stx1/stx2 positive E. coli showing multi-drug resistance. Information obtained from the study will be used for modelling the public health risk posed by milkborne pathogens in PDBM, which in many cases is consumed by the poor and vulnerable population.

show abstract

“…They are not only detected in humans (Pitout et al, 2009), but also in a wide range of animal species ranging from companion animals (Carattoli et al, 2005) to food animals (Horton et al, 2011). The emergence of ESBLs in the food-producing animals and in food products like meat, fish and raw milk have been reported from different parts of globe (Geser et al, 2012). The poor hygienic practices further enhances the possibility of transfer ESBL producer strains to the GIT of consumers (Overdevest et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characterization of extended-spectrum b-Lactamases (blaTEM) producing Escherichia coli isolated from humans and foods of animal origin in Chhattisgarh, India

Dewangan

Shakya

Patyal

et al. 2016

IJAR

View full text Add to dashboard Cite

The present study describes the prevalence of extended spectrum b-lactamases (ESBL) producing E. coli in raw chevon, milk and human samples in different districts of Chhattisgarh state. A total of 330 samples comprising of chevon (n=126), raw milk (n=104), human urine and stool (n=100) were collected from Bilaspur, Durg, Raipur, Rajnandgaon and Dhamtari districts of Chhattisgarh and processed for isolation of E. coli. The biochemically confirmed E. coli isolates were further screened of for the presence of bla TEM gene by PCR amplification. Analysis of samples indicated an overall prevalence of 31.52%. The highest prevalence of E. coli was recorded in fresh chevon samples (38.09%) followed by human urine samples (37.14%), human stool samples (30%) followed by milk samples (20.19%). In -vitro antibiotic sensitivity test of E. coli isolates revealed that all isolates to be highly sensitive towards imipenem, gentamicin, ciprofloxacin, amoxyclav, ampicillin, oxytetracyclin. The highest numbers of E. coli isolates were found resistant to erythromycin, cefotaxim, ceftazidime, cephalexin and cifixime. The 49 E. coli isolates were found to have Multiple Antibiotic Resistance (MAR) index more than 0.2, thus indicating indiscriminate use of antimicrobials. The 44 (42.3%) isolates were identified as presumptive ESBL producers and out of them 39.4% isolates were found to harbour the bla TEM gene on their plasmid DNA indicating the presence of multidrug resistant ESBL producing E. coli in foods of animal origin and human samples.

show abstract

Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk

Cited by 300 publications

References 39 publications

Antimicrobial Resistance in ESBL-Producing Escherichia coli Isolated from Layer and Pig Farms in Thailand

Antimicrobial Resistance in ESBL-Producing Escherichia coli Isolated from Layer and Pig Farms in Thailand

Characterization of Escherichia coli and other Enterobacteriaceae in producer-distributor bulk milk

Prevalence and molecular characterization of extended-spectrum b-Lactamases (blaTEM) producing Escherichia coli isolated from humans and foods of animal origin in Chhattisgarh, India

Contact Info

Product

Resources

About