Occurrence and analysis of irp2 virulence gene in isolates of Klebsiella pneumoniae and Enterobacter spp. from microbiota and hospital and community-acquired infections

Lópes, Ana Catarina de Souza; Rodrigues, Juliana Falcão; Cabral, Adriane Borges; Silva, Maíra Espíndola da; Leal, Nilma Cintra; Silveira, Vera Magalhães da; Júnior, Marcos Antônio de Morais

doi:10.1016/j.micpath.2016.04.018

Cited by 16 publications

(12 citation statements)

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“…This phenomenon characterizes the high pathogenicity of K. pneumoniae strains. These virulence genes were also predominant in the K. pneumoniae strains isolated from hospital-acquired infections in China,35 UK,36 France,37 and Brazil 38. Type 1 fimbriae is the most frequent adhesive factor in K. pneumoniae strains.…”

Section: Discussionmentioning

confidence: 92%

Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the Klebsiella pneumoniae strains isolated from different types of hospital-acquired infections

Ranjbar

Kelishadrokhi

Chehelgerdi

2019

IDR

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Purpose: Virulent and resistant Klebsiella pneumoniae strains are considered as one of the most significant causes of hospital-acquired infections. The present investigation was done to study the distribution of virulence factors, capsule serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the K. pneumoniae strains isolated from hospital-acquired infections. Patients Materials and methods: Two hundred and sixty different types of hospital-acquired infections were collected and cultured. Antibiotic resistance pattern of K. pneumoniae isolates and their molecular characterization were studied using disk diffusion and PCR, respectively. Results: One hundred and fifty out of 260 (44.22%) hospital-acquired infections harbored K. pneumoniae . Urine samples (63.75%) had the highest prevalence of K. pneumoniae , while wound (33.33%) had the lowest. K. pneumoniae strains harbored the highest prevalence of resistance against ampicillin (100%), cefuroxime (100%), amoxicillin/clavulanic acid (95.65%) and ceftazidime (95.52%). FimH-1 (93.04%), traT (92.17%), mrkD (84.34%), and entB (80.86%) were the most commonly detected virulence genes. AcrAB (96.52%) and tolC (85.21%) were the most commonly detected antibiotic resistance genes. Prevalence of ompK35 and ompK36 virulence genes were 75.65% and 79.13%, respectively. Prevalence of K1 and K2-positive serotypes were 27.82% and 6.96%, respectively. Conclusions: High prevalence of resistance against several types of antibiotics and simultaneous presence of some virulence factors and multi-drug resistance genes pose an important public health issue.

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Section: Discussionmentioning

confidence: 92%

Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the Klebsiella pneumoniae strains isolated from different types of hospital-acquired infections

Ranjbar

Kelishadrokhi

Chehelgerdi

2019

IDR

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“…Among these genes, the irp2 gene has been identified in Enterobacter spp. (147). Finally, E. cloacae complex strains can harbor curli-encoding genes involving host cell adhesion and invasion.…”

Section: Clinical Aspects Pathogenicitymentioning

confidence: 99%

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

2019

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SUMMARY The genus Enterobacter is a member of the ESKAPE group, which contains the major resistant bacterial pathogens. First described in 1960, this group member has proven to be more complex as a result of the exponential evolution of phenotypic and genotypic methods. Today, 22 species belong to the Enterobacter genus. These species are described in the environment and have been reported as opportunistic pathogens in plants, animals, and humans. The pathogenicity/virulence of this bacterium remains rather unclear due to the limited amount of work performed to date in this field. In contrast, its resistance against antibacterial agents has been extensively studied. In the face of antibiotic treatment, it is able to manage different mechanisms of resistance via various local and global regulator genes and the modulation of the expression of different proteins, including enzymes (β-lactamases, etc.) or membrane transporters, such as porins and efflux pumps. During various hospital outbreaks, the Enterobacter aerogenes and E. cloacae complex exhibited a multidrug-resistant phenotype, which has stimulated questions about the role of cascade regulation in the emergence of these well-adapted clones.

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“…In addition, the search for virulence factors detected genes related to the phenolate siderophore Yersiniabactin ( ybtA, ybtE, ybtP, ybtQ, ybtS, ybtT, ybtU, ybtX ), irp 1, irp 2 and fyuA . The irp2 gene is observed in strains derived from urinary infections, bacteremia and other sources, and is considered an important virulence factor for extraintestinal infection establishment ( 12 ). Furthermore, mannose-specific adhesin subunit of type 3 fimbriae mrk ABCDF and mrk HIJ loci, able to mediate bacterial adhesion and biofilm formation for several surface structures ( 13 ) were also detected (Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

A Fatal Bacteremia Caused by Hypermucousviscous KPC-2 Producing Extensively Drug-Resistant K64-ST11 Klebsiella pneumoniae in Brazil

et al. 2018

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We report a fatal bacteremia caused by Klebsiella pneumoniae in a 60–70-year-old patient from Brazil. The genomic analysis of three isolates (from blood culture, nasal and anal swabs) showed that the bacteremia was caused by a KPC-2 producing extensively drug-resistant K64-ST11 hypermucousviscous K. pneumoniae (hmKP) harboring several virulence and antimicrobial resistance genes. Although the isolates did not present virulence markers associated with hypervirulent K. pneumoniae (hvKP), they showed invasion and toxicity to epithelial Hep-2 cells; resistance to cell microbicidal mechanisms; and blood and human serum survival, evidencing their pathogenic potential. This study highlights the risk of infection caused by hmKp strains not characterized as hvKP as well as the clinical implications and difficulty of treatment, especially in elderly or immunocompromised patients.

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Occurrence and analysis of irp2 virulence gene in isolates of Klebsiella pneumoniae and Enterobacter spp. from microbiota and hospital and community-acquired infections

Cited by 16 publications

References 20 publications

<p>Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the<em> Klebsiella pneumoniae</em> strains isolated from different types of hospital-acquired infections</p>

<p>Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the<em> Klebsiella pneumoniae</em> strains isolated from different types of hospital-acquired infections</p>

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

A Fatal Bacteremia Caused by Hypermucousviscous KPC-2 Producing Extensively Drug-Resistant K64-ST11 Klebsiella pneumoniae in Brazil

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