Occurance ofStaphylococcus nepalensisstrains in different sources including human clinical material

Abstract

Five isolates of coagulase-negative staphylococci were obtained from human urine, the gastrointestinal tract of squirrel monkeys, pig skin and from the environment. All key biochemical characteristics of the tested strains corresponded with the description of Staphylococcus xylosus species. However, partial 16S rRNA gene sequences obtained from analysed strains corresponded with those of Staphylococcus nepalensis reference strains, except for two strains which differed in one residue. Ribotyping with EcoRI and HindIII restriction enzymes, whole cell protein profile analysis performed by SDS-PAGE and SmaI macrorestriction analysis were used for more precise characterization and identification of the analysed strains. Obtained results showed that EcoRI and HindIII ribotyping and whole cell protein fingerprinting are suitable and reliable methods for the differentiation of S. nepalensis strains from the other novobiocin resistant staphylococci, whereas macrorestriction analysis was found to be a good tool for strain typing. The isolation of S. nepalensis is sporadic, and according to our best knowledge this study is the first report of the occurrence of this species in human clinical material as well as in other sources.

Impacts of Poultry House Environment on Poultry Litter Bacterial Community Composition

“…BLAST analysis of this sequence found 94% homology to an uncultured Firmicute isolate from a DGGE band produced from a chicken litter sample [76]. There were also a number of BLAST hits to Staphylococcus nepalensis isolated from the GI tracts of monkey and pigs [4]. In the same study, Novakova et al [4] found that it was impossible to differentiate between S. xylosus and S. nepalensis by biological tests alone and additional 16S rRNA gene sequencing was required to differentiate these two species.…”

“…There were also a number of BLAST hits to Staphylococcus nepalensis isolated from the GI tracts of monkey and pigs [4]. In the same study, Novakova et al [4] found that it was impossible to differentiate between S. xylosus and S. nepalensis by biological tests alone and additional 16S rRNA gene sequencing was required to differentiate these two species. This difficulty in strain identification calls into question previous cultivation-based findings showing S. xylosus to be the dominant Staphylococcus species in poultry litter [30].…”

“…Previous molecular genetic studies detected a diverse range of antibiotic resistance genes [4] and both human and avian pathogens [5] within poultry litter samples. However, none of these studies has investigated possible links between the composition of litter microbial communities and the incidence of disease within a poultry house.…”

Microbiome analysis reveals the abundance of bacterial pathogens in Rousettus leschenaultii guano

“…The main genus among these is Staphylococcus . It included Staphylococcus aureus (a RG-2 organism), Staphylococcus nepalensis ( 13 ) and Staphylococcus lentus ( 49 ), which are well-known to cause various infections in humans as well as in other animals5758. Therefore, it may pose a threat to the herds of sheep and goats grazing nearby bats’ roosting sites.…”

“…Barcode specific fastq files were processed using mothur pipeline57 to obtain fasta and quality file. These two types of the files were quality filtered using following conditions: Size 150–200 bp, homo-polymer max.…”