Viral and bacterial pathogens are a significant economic concern to the US broiler industry and the ecological epicenter for poultry pathogens is the mixture of bedding material, chicken excrement and feathers that comprises the litter of a poultry house. This study used high-throughput sequencing to assess the richness and diversity of poultry litter bacterial communities, and to look for connections between these communities and the environmental characteristics of a poultry house including its history of gangrenous dermatitis (GD). Cluster analysis of 16S rRNA gene sequences revealed differences in the distribution of bacterial phylotypes between Wet and Dry litter samples and between houses. Wet litter contained greater diversity with 90% of total bacterial abundance occurring within the top 214 OTU clusters. In contrast, only 50 clusters accounted for 90% of Dry litter bacterial abundance. The sixth largest OTU cluster across all samples classified as an Arcobacter sp., an emerging human pathogen, occurring in only the Wet litter samples of a house with a modern evaporative cooling system. Ironically, the primary pathogenic clostridial and staphylococcal species associated with GD were not found in any house; however, there were thirteen 16S rRNA gene phylotypes of mostly Gram-positive phyla that were unique to GD-affected houses and primarily occurred in Wet litter samples. Overall, the poultry house environment appeared to substantially impact the composition of litter bacterial communities and may play a key role in the emergence of food-borne pathogens.
Panelists Myron Krueger Independent Consultant Ed Tannenbaum Independent Artist Don Ritter Concordia University This panel focuses on and addresses the distinctions made between computer art, interactive art, and artificial and virtual realities. Panelists consider how the computer, as a symbol-manipulating, all-purpose machine, is a tool that changes the way art is created and experienced. Panelists argue that this view implies interactivity and possibly trivializes static paintbox computer art by changing the viewer's role from passive observer to active participant. Interactivity is discussed as a step toward artificial or virtual realities and a means to define new possibilities in real-time performance.
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