This paper presents a multi-dimensional measure of organisational climate covering two domains of health behavior (healthy eating, physical exercise). To develop and validate the climate measure, we conducted three independent studies. In Study 1, items were generated and evaluated in expert ratings and confirmatory factor analyses (N 5 483 employees), resulting in a total of 24 items comprising three dimensions (values and expectations, organisational practices, communication) within the domains of healthy eating and physical exercise. Study 2 crossvalidated the factor structure of this measure of organisational health behavior climate (OHBC), and examined its nomological net. Based on data from 464 employees, we found significant correlations among OHBC and other dimensions of organisational climate (e.g. employee welfare, safety climate), healthy eating and exercise identity, eating behavior, and body mass index (BMI). Study 3 demonstrated within-organisation agreement and between-organisation differences in OHBC, and replicated the factor structure with a multi-level data set (N 5 1,077 employees from 48 organisations). This new measure assesses organisational eating climate and exercise climate in a comprehensive way, opening up multiple avenues for future research and evaluation studies in organisations.