Occupational Exposure to PAHS - Biological Monitoring of Hydroxylated Metabolites

Mannschreck, C.; Gündel, J.; Angerer, Jürgen

doi:10.1080/10406639608544644

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Long-term monitoring of average urinary 1-OHpyrene, as part of epidemiological studies of cancer mortality, is needed to establish an accurate healthbased limit value of this biomarker for occupational PAH exposure. Carbon electrode worker 0.5-62 1.0-117 Bentsen et al (1998), Mannschreck et al (1996), Petry et al (1996), Ovrebo et al (1994), Ferreira et al (1994) and Angerer et al (1997) Coke oven operator 0.2-176 0.4-334 Strunk et al (2002), Wu et al (1998a, b), Kuljukka et al (1997), Mielzynska et al (1997), Pyy et al (1997), VanRooij et al (1993, Zhao et al (1990), Ferreira et al (1994) and Moen and Ovrebo (1997) …”

Section: Discussionmentioning

confidence: 96%

Comparison of 1-hydroxypyrene exposure in the US population with that in occupational exposure studies

Huang

Grainger

Patterson

et al. 2004

Int Arch Occup Environ Health

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Urine samples collected in 1999 and 2000 as part of the National Health and Nutrition Examination Survey (NHANES) were analyzed for 14 monohydroxy polycyclic aromatic hydrocarbons (PAHs), and, for the first time, reference range values were calculated for these metabolites in the US population. Pyrene is a major component of most PAH mixtures and often is used as a surrogate for total PAH exposure. We detected 1-hydroxypyrene (1-OHpyrene), a metabolite of pyrene, in more than 99% of the samples. The overall geometric mean concentration for 1-OHpyrene in the USA was 79.8 ng/l, with a 95% confidence interval (CI) of 69.0-92.2 ng/l. The overall geometric mean creatinine-adjusted urinary 1-OHpyrene levels in the USA was 74.2 ng/g creatinine (0.039 micromol/mol), with a 95% CI of 64.1-85.9 ng/g creatinine (0.034-0.046 micromol/mol). There were no statistically significant differences among age, gender, or race/ethnicity groups. Adult smokers in the USA have urinary 1-OHpyrene levels three times higher than those of non-smokers. This difference was statistically significant. In this paper, we compare the reference range of urinary 1-OHpyrene levels with levels reported from various occupations by other researchers.

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Section: Discussionmentioning

confidence: 96%

Comparison of 1-hydroxypyrene exposure in the US population with that in occupational exposure studies

Huang

Grainger

Patterson

et al. 2004

Int Arch Occup Environ Health

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“…32 P-post-labeling assays can detect total aromatic DNA adducts, giving a measure of exposure to total PAH (4,11,12). Quantification of various PAH in urine has been suggested to be a useful biomarker, either after reversed metabolism (enzymatic hydrolysis) to parent PAH (13) or by determining a variety of hydroxylated PAH (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Urinary PAH Metabolites as Biomarkers of Exposure in Aquatic Environments

Fillmann

Watson

Howsam

et al. 2004

Environ. Sci. Technol.

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While immunoassays have been extensively applied to evaluate environmental contamination, to date they have rarely been used for the analysis of biological fluids outside of human medicine. These media are important because pollutants such as polycyclic aromatic hydrocarbons (PAHs) and their metabolites become concentrated in tissues, body fluids, and excreta, thereby offering a measure of exposure to biologically available contaminants. Such analyses also provide a nondestructive tool for monitoring exposure. Crabs (Carcinus maenas) were exposed to phenanthrene and pyrene (separately) at concentrations ranging from 0 to 200 microg L(-1). After 48 h, urine samples were taken and analyzed by immunoassay and UV-fluorescence spectrophotometry. Urinary levels (calibrated against hydroxylated metabolites) proved to be dose dependent for both compounds, and good agreementwas demonstrated between the immunoassay and the fluorescence techniques. The cross reactivity of the immunochemical technique (ELISA) for pyrene and hydroxy-metabolites was lower than for phenanthrene. HPLC analyses demonstrated that urine from the crabs exposed to pyrene contained mainly conjugate PAH metabolites whose concentrations (the sum of the four main pyrene metabolites/conjugates) showed very good agreement with the ELISA (r2 > 0.94) and fluorescence (r2 > 0.91) data. Environmental samples were also analyzed by ELISA and UV-fluorescence, and both techniques detected PAH (mainly petrogenic) contamination in the urine samples from a polluted harbor. These data demonstrate the potential of urine analyses by ELISA and UV-fluorescence to measure exposure of crabs to PAH.

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“…180 Angerer et al m Angerer et al m Angerer et a/. 180 Mannschreck et al 20 Bentsen-Farmen et al 183 Bentsen-Farmen et al 183 Angerer etal. m Angerer et a/.…”

Section: -Hydroxypyrene and Its Glucuronideunclassified

Biomonitoring of Polycyclic Aromatic Hydrocarbons — Human Exposure

Seidel¹

2005

The Carcinogenic Effects of Polycyclic Aromatic Hydrocarbons

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Occupational Exposure to PAHS - Biological Monitoring of Hydroxylated Metabolites

Cited by 11 publications

References 10 publications

Comparison of 1-hydroxypyrene exposure in the US population with that in occupational exposure studies

Comparison of 1-hydroxypyrene exposure in the US population with that in occupational exposure studies

Urinary PAH Metabolites as Biomarkers of Exposure in Aquatic Environments

Biomonitoring of Polycyclic Aromatic Hydrocarbons — Human Exposure

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