Obtention de plantes haploïdes par culture <i>in vitro</i> d'ovaires et d'ovules non fécondés de tournesol (Helianthus annuus L.)

Gélébart, Pascal; San, L.H.; Martin, M.

doi:10.1051/agro:19870202

Cited by 27 publications

(6 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gynogenically derived haploid plants can be obtained in monocotyledonous species such as onion (Bohanec and Jakse 1999), leek (Schum et al 1993), shallot (Cohat 1994) and maize (Bordes et al 1997) as well as in dicotyledonous species such as sugarbeet (Ferrant and Bouharmont 1994), sunflower (Gelebart and San 1987) and gerbera (Tosca et al 1995). However, only a few results have been published on haploid induction in Cucurbitaceae.…”

Section: Introductionmentioning

confidence: 99%

Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber ( Cucumis sativus L.)

Gémes-Juhász¹,

Balogh

Ferenczy

et al. 2002

Plant Cell Reports

View full text Add to dashboard Cite

Gynogenic plants of pickling cucumber were successfully produced by in vitro culture from unpollinated ovules. Haploids and spontaneous doubled haploids originated directly through embryogenesis. The cucumber ovaries were placed on induction media containing thidiazuron and cultured in the dark for 2-5 days, at 24°C, 28°C or 35°C. After induction, the material was transferred to regeneration media containing α-naphthaleneacetic acid and 6-benzylaminopurine. Results from the regeneration test indicated that the heat treatment increased the efficiency of gynogenesis in the optimal developmental stage of the female gametophyte. The highest number of embryos occurred following the35°C induction treatment. The maximum frequency of gynogenesis was 18.4%, while maximum plant regeneration was 7.1%. The flow cytometry analysis showed that 87.7% of the regenerants were haploid. On the basis of histological studies carried out on the female gametophyte, the best developmental stage for haploid induction seems to be the cellularization stage of embryo-sac formation, when the nuclei are already in position, the membranes have sometimes developed, and the cells are quite uniform in shape and structure.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber ( Cucumis sativus L.)

Gémes-Juhász¹,

Balogh

Ferenczy

et al. 2002

Plant Cell Reports

View full text Add to dashboard Cite

show abstract

“…Nowadays, homozygous lines can be quickly obtained by in vitro androgenesis or gynogenesis in many species. Haploid plants have been obtained through gynogenesis in Hordeum vulgare [9], Oryza sativa [1], Gerbera jamesonii [10], Nicotiana tabacum [13], Triticum aestivum [12], Beta vulgaris [5], Zea mays [11], Helianthus annuus [3]. In Allium cepa, culture of unfertilized ovules and ovaries has already been tried [4] but the experiments failed to produce plants.…”

Section: Introductionmentioning

confidence: 99%

Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules

Campion¹,

Alloni²

1990

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Haploid plants have been obtained from unpollinated ovules of onion cultured in vitro as here reported for the first time. Callus was not formed at any step of the process and the young developing embryos sprouted by splitting the ovules. From a total of about 26 000 cultured ovules, 13 embryos were obtained and 7 of them developed into plants. Cytological observation of root apexes showed that 3 embryos out of 7 regenerated haploid plants (8 chromosomes). The yield of embryo induction was quite low (0.28% the best treatment) so further experiments are in progress to increase it.

show abstract

“…Haploids of sunflower have already been obtained via anther culture (ALISSA et al 1985, BoHOROvA et al 1985, Mix 1985, MEZZAROBBA and JoNARD 1986 as well as by in vitro culture of nonfertilized ovaries and ovules (GELEBART and SAN 1987). The major aim of our study was to compare different methods of sunflower anther culture described in the literature and to adapt one of them to relevant, valuable sunflower genotypes.…”

mentioning

confidence: 99%

Shoot Regeneration from Anther Culture of Sunflower (Helianthus annuus) and Some Interspecific Hybrids as Affected by Genotype and Culture Procedure

Gürel

Nichterlein²,

Friedt³

1991

Plant Breeding

View full text Add to dashboard Cite

Anther culture has been demonstrated to be an applicable technique for the development of doubled haploid, i. e. homozygous lines of many crop species. In some species, androgenetic doubled haploids have already been shown to be a useful tool for breeding. However, anther culture results in sunflower have been rather unsatisfactory up to now.As in other species, anther culture response of sunflower {Helianthus sp.) is strongly affected by physical, nutritional, physiological and genetical factors. By testing a number of different culture parameters, i. e. donor plant stages, culture media and conditions, and appropriate schedule could be worked out for the successful regeneration of shoots -at least for a number of sunflower lines and interspecific hybrids.

show abstract

Obtention de plantes haploïdes par culture in vitro d'ovaires et d'ovules non fécondés de tournesol (Helianthus annuus L.)

Cited by 27 publications

References 1 publication

Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber ( Cucumis sativus L.)

Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber ( Cucumis sativus L.)

Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules

Shoot Regeneration from Anther Culture of Sunflower (Helianthus annuus) and Some Interspecific Hybrids as Affected by Genotype and Culture Procedure

Contact Info

Product

Resources

About