OBTENÇÃO DE HIDROLISADOS DE CONCENTRADO PROTEICO DE SORO DE LEITE COM ATIVIDADE INIBITÓRIA DA ENZIMA CONVERSORA DE ANGIOTENSINA: AÇÃO DAS PROTEASES DO Bacillus licheniformis E DO Aspergillus oryzae

SILVESTRE, M. P. C.; Morais, Harriman Aley; SILVA, Viviane Dias Medeiros; SILVA, Mauro Ramalho

doi:10.5212/publ.exatas.v.17i2.0003

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…However, the optimal hydrolysis conditions vary depending on the type of whey and enzyme used, in addition, the hydrolysis conditions (temperature, pH, E:S ratio, and hydrerolysis time). In a study performed by da Silva et al (2009), the best conditions obtained for WPC hydrolysis by Aspergillus oryza protease were E:S 1:100, pH 7, 50°C, 5 h, while in another study for Silva et al (2010) outstanding hydrolysis conditions for hydrolyzing WPC by using papain and pancreatin were (E:S 2:100, pH 7, 55°C, 5 h) and (3:100; pH 7, 50°C, 5 h) respectively. Also, in another study carried out by Mota et al (2006), the best conditions received for β-lg hydrolysis for ve h by pancreatic trypsin with an activity of 1020 BAEE units/mg protein were pH 8, 50°C.…”

Section: Optimization Of Wpc Hydrolysismentioning

confidence: 96%

Whey Protein Concentrate Hydrolyzed by Microbial Protease: Process Optimization and Evaluation of Its Dipeptidyl Peptidase Inhibitory Activity

Haj Mustafa,

Soleimanian-Zad,

Albukhaty

2023

Waste Biomass Valor

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Whey proteins are abundant in peptides that possess various biological activities. In order to enhance the biological properties of protein hydrolysates, it is essential to optimize the conditions of the hydrolysis process as much as possible. Firstly, we have determined the optimal conditions for hydrolysis of whey protein concentrate (WPC) by by ltered trypsin-like protease (FTLP) in vitro conditions. Then, the ability of obtained whey protein hydrolysates against inhibit dipeptidyl peptidase-4 (DiPP4) in vitro conditions was examined. The optimum point for WPC hydrolysis by FTLP with the predicted optimal level of Degree of hydrolysis (DH, 42.9 %) was at an E:S ratio of 5:100 (w/w), 8.6 h, and a temperature of 40 °C. The factual DH under ideal conditions was 42.04 %, indicating the e ciency of the selected model (P ≤ 0.05).The ndings indicated that hydrolysates of WPC generated by FTLP, including both the unfractionated section and the fractions obtained via ultra ltration using 10-and 5-kDa cut-off membranes, exhibited anti-diabetic characteristics. However, the fractions exhibited greater inhibitory effects against the DiPP4 enzyme, with IC50 values of 1.98, 1.19, and 0.9 mg/mL for the unfractionated section, 10-kDa fraction, and 5-kDa fraction, respectively. Moreover, the results indicated that probiotic L. plantarum subsp. plantarumPTCC 1896 or its components may provide opportunities for future management of typediabetes by inhibiting DiPP4.

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Section: Optimization Of Wpc Hydrolysismentioning

confidence: 96%

Whey Protein Concentrate Hydrolyzed by Microbial Protease: Process Optimization and Evaluation of Its Dipeptidyl Peptidase Inhibitory Activity

Haj Mustafa,

Soleimanian-Zad,

Albukhaty

2023

Waste Biomass Valor

View full text Add to dashboard Cite

show abstract

Optimization of hydrolysis of whey protein concentrate using trypsin-like microbial protease and evaluation of dipeptidyl peptidase inhibitory activity of the obtained hydrolyzed product

Mustafa

Soleimanian‐Zad

Albukhaty

2023

Preprint

View full text Add to dashboard Cite

Whey proteins are abundant in peptides that possess various biological activities. In order to enhance the biological properties of protein hydrolysates, it is essential to optimize the conditions of the hydrolysis process as much as possible. Firstly, we have determined the optimal conditions for hydrolysis of whey protein concentrate (WPC) by by filtered trypsin-like protease (FTLP) in vitro conditions. Then, the ability of obtained whey protein hydrolysates against inhibit dipeptidyl peptidase-4 (DiPP4) in vitro conditions was examined. The optimum point for WPC hydrolysis by FTLP with the predicted optimal level of Degree of hydrolysis (DH, 42.9 %) was at an E:S ratio of 5:100 (w/w), 8.6 h, and a temperature of 40 °C. The factual DH under ideal conditions was 42.04 %, indicating the efficiency of the selected model (P ≤ 0.05). The findings indicated that hydrolysates of WPC generated by FTLP, including both the unfractionated section and the fractions obtained via ultrafiltration using 10- and 5-kDa cut-off membranes, exhibited anti-diabetic characteristics. However, the fractions exhibited greater inhibitory effects against the DiPP4 enzyme, with IC50 values of 1.98, 1.19, and 0.9 mg/mL for the unfractionated section, 10-kDa fraction, and 5-kDa fraction, respectively. Moreover, the results indicated that probiotic L. plantarum subsp. plantarumPTCC 1896 or its components may provide opportunities for future management of type-Ⅱ diabetes by inhibiting DiPP4.

show abstract

OBTENÇÃO DE HIDROLISADOS DE CONCENTRADO PROTEICO DE SORO DE LEITE COM ATIVIDADE INIBITÓRIA DA ENZIMA CONVERSORA DE ANGIOTENSINA: AÇÃO DAS PROTEASES DO Bacillus licheniformis E DO Aspergillus oryzae

Cited by 2 publications

References 27 publications

Whey Protein Concentrate Hydrolyzed by Microbial Protease: Process Optimization and Evaluation of Its Dipeptidyl Peptidase Inhibitory Activity

Whey Protein Concentrate Hydrolyzed by Microbial Protease: Process Optimization and Evaluation of Its Dipeptidyl Peptidase Inhibitory Activity

Optimization of hydrolysis of whey protein concentrate using trypsin-like microbial protease and evaluation of dipeptidyl peptidase inhibitory activity of the obtained hydrolyzed product

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