Obtaining antimicrobial peptides by controlled peptic hydrolysis of bovine hemoglobin

Composition and biological activities of slaughterhouse blood from red deer, sheep, pig and cattle

“…While the plasma and red blood cell fractions in this study did not demonstrate strong antimicrobial activity, it has been documented that antimicrobial peptides can be derived from the haemoglobin fragments of red blood cells, for example through peptic hydrolysis of bovine haemoglobin . Native ovine haemoglobin tetramer displayed no activity at the highest tested concentration of 30 mg mL −1 , but the separation of subunits at 0.5–2.0 mg mL −1 provided moderate activity against E. coli and S. aureus .…”

Generation of bioactive peptide hydrolysates from cattle plasma using plant and fungal proteases

“…Antimicrobial peptides derived from the hemoglobin fragments of animal red blood cells have been reported, for example when bovine hemoglobin was hydrolysed using pepsin (Adje, Balti, Kouach, Dhulster, Guillochon, & Nedjar-Arroume, 2011;Froidevaux, Krier, Nedjar-Arroume, Vercaigne-Marko, Kosciarz, Ruckebusch, et al, 2001), or from the neutrophils (white blood cells) separated from animal blood (Anderson & Yu, 2008;Treffers, Chen, Anderson, & Yu, 2005;Wessely-Szponder, Majer-Dziedzic, & Smolira, 2010).…”

Production of an antimicrobial peptide derived from slaughterhouse by-product and its potential application on meat as preservative

“…About cruor, it is the fraction which gives blood its red color, representing 45% of the whole blood, and it contains mainly hemoglobin, a globular tetramer (64,500 Da) composed of four protoporphyrin groups, each including an iron atom in their center. This protein is broadly described as a rich source of bioactive peptides after enzymatic hydrolysis (Adje et al, 2011;Gomes et al, 2010;NedjarArroume et al, 2008). The most described activity is antimicrobial (Choisnard et al, 2002;Daoud et al, 2005;Froidevaux et al, 2001).…”