Observations on Traditional Phytotherapy among the Inhabitants of Lahaul Valley through Amchi System of Medicine—A Cold Desert Area of Himachal Pradesh in North Western Himalayas, India

Sharma, Parveen; Thakur, Surender Kumar; Manuja, S.; Rana, Rumana; Kumar, Pardeep; Sharma, Sanjay; Chand, J. N.; Singh, Ashok K.; Katoch, Krishan Kumar

doi:10.4236/cm.2011.23016

Cited by 26 publications

(10 citation statements)

References 7 publications

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“…The plant is very popularly used amongst many tribes across India and in the regions falling around the Himalayan belt for treating diseases. Selinum vaginatum is popular amongst Gujjar tribe, Jaad Bhotiya Community Bhotia tribe, Lahaul valley, tribals from Parbati valley, district Chamba of Western Himalaya, Bhotia tribe of Mana village, Nanda Devi Biosphere reserve, local inhabitants of Banaun, tribal inhabitants from Garhwal, Chamoli, Uttarakhand, South India and many other regions ( Arya et al., 2018 ; Balodi et al., 2018 ; Chauhan, 2014 ; Gairola et al., 2014 ; Garbyal et al., 2005 ; Kumar et al., 2019 ; Malik et al., 2015 ; Nand and Naithani, 2018 ; Radha and Puri, 2019 ; Radha et al., 2019 ; Rana et al., 2010 , 2013 , 2019 ; Rana and Samant, 2011 ; Semwal et al., 2010 ; Sharma et al., 2004 , 2011 , 2013 ; Singh et al., 2009 ; Thakur et al., 2017 ). However, the effect of Selinum vaginatum in diabetic complications has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Anti-diabetic, diabetic neuropathy protective action and mechanism of action involving oxidative pathway of chlorogenic acid isolated from Selinum vaginatum roots in rats

Saraswat

Sachan

Chandra

2020

Heliyon

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Phytopharmaceuticals have always reported vital roles in the field of medicine hence the need to investigate safe and efficient drugs for treating metabolic disorders is very significant. Roots of Selinum vaginatum have therapeutic benefits and are widely used by the people of the Rohtang region for treating diabetes and its associated complications. The present study focusses on the isolation of the bioactive from the S. vaginatum roots for estimating acute toxicity studies, anti-diabetic and diabetic neuropathy protective action along with the mechanism of action in STZ induced Wistar rats. The Selinum vaginatum roots were collected from the Rohtang region, Himalayas. Chlorogenic acid was isolated and underwent identification by UV, HPLC, 1 H NMR, C13 NMR, Mass, and FTIR spectroscopy methods. Chlorogenic acid was dosed at 10 and 20 mg/kg to observe the effects on experimentally induced diabetes and with time generated diabetic neuropathic complications. Biomarkers TNF-α, superoxide dismutase, nitrosative stress, lipid peroxide profile, and membrane-bound inorganic phosphate were analyzed. Histopathological evaluation of the liver and sciatic nerve was performed for all groups. Parameters like blood glucose levels, body weight, food intake, Thermal Hyperalgesia, Writhing, Cold Hyperalgesia Responses, Mechanical hyperalgesia, Grip Strength, Spontaneous Locomotor (Exploratory) Test, Neuromuscular Coordination tests, and lipid profile analysis showcased the anti-diabetic and diabetic neuropathy protective action of the drug. Inflammation, degradation, and necrosis were found to be reduced in the liver and sciatic nerve cells of treated groups. All the biomarkers used to analyze the oxidative pathway were significantly replenished indicates that chlorogenic acid produces these effects through this pathway.

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Section: Introductionmentioning

confidence: 99%

Anti-diabetic, diabetic neuropathy protective action and mechanism of action involving oxidative pathway of chlorogenic acid isolated from Selinum vaginatum roots in rats

Saraswat

Sachan

Chandra

2020

Heliyon

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“…O. hispida is used as a medicinal herb [ 30 , 31 ] exhibiting various biological properties including antibacterial activity [ 32 ]. The plant O. hispida has been reported to be used as blood purifier and for cuts, swells, wounds [ 33 ]. And it has also been reported for the treatment of abdominal ulcers, hair problems, bladder and kidney stones and rheumatism [ 34 ], pneumonia, typhoid fever and also used for dyeing hairs [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Antitumor evaluation of two selected Pakistani plant extracts on human bone and breast cancer cell lines

Engel

Ali

Adamus

et al. 2016

BMC Complement Altern Med

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BackgroundThe medicinal plants Vincetoxicum arnottianum (VSM), Berberis orthobotrys (BORM), Onosma hispida (OHRM and OHAM) and Caccinia macranthera (CMM) are used traditionally in Pakistan and around the world for the treatment of various diseases including cancer, dermal infections, uterine tumor, wounds etc. The present study focuses on the investigation of the selected Pakistani plants for their potential as anticancer agents on human bone and breast cancer cell lines in comparison with non-tumorigenic control cells.MethodsThe antitumor evaluation was carried out on human bone (MG-63, Saos-2) and breast cancer cell lines (MCF-7, BT-20) in contrast to non-tumorigenic control cells (POB, MCF-12A) via cell viability measurements, cell cycle analysis, Annexin V/PI staining, microscopy based methods as well as migration/invasion determination, metabolic live cell monitoring and western blotting.ResultsAfter the first initial screening of the plant extracts, two extracts (BORM, VSM) revealed the highest potential with regard to its antitumor activity. Both extracts caused a significant reduction of cell viability in the breast and bone cancer cells in a concentration dependent manner. The effect of VSM is achieved primarily by inducing a G2/M arrest in the cell cycle and the stabilization of the actin stress fibers leading to reduced cell motility. By contrast BORM’s cytotoxic properties were caused through the lysosomal-mediated cell death pathway indicated by an upregulation of Bcl-2 expression.ConclusionsThe antitumor evaluation of certain medicinal plants presented in this study identified the methanolic root extract of Berberis orthobotrys and the methanolic extract of Vincetoxicum arnottianum as promising sources for exhibiting the antitumor activity. Therefore, the indigenous use of the herbal remedies for the treatment of cancer and cancer-related diseases has a scientific basis. Moreover, the present study provides a base for phytochemical investigation of the plant extracts.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1215-9) contains supplementary material, which is available to authorized users.

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“…It has been found that dried root powder of CT cure severe joint pains. [10,11] It is also found effective in curing headache, stomach problems, measles, and boils. There is very scarce literature present regarding this plant, and little information is available about the biological effects of its constituents.…”

Section: Abstract a R T I C L E I N F Omentioning

confidence: 99%

Evaluation of Mutagenic and Antimutagenic Activity of Methanol Extract of Cousinia thomsonii against Cyclophosphamide

Khan

Bashir

Gulzar

et al. 2020

Int. J. Pharm. Sci. Drug Res.

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The present study was conducted to evaluate the mutagenic and antimutagenic potential of Cousinia thomsonii (CT) extract in bone marrow cells of male wistar rats using some important parameters like micronucleated polychromatic erythrocyte (MnPCE), mitotic index (MI), chromosomal aberrations (CA) and polychromatic erythrocyte to normochromatic erythrocyte ratio (PCE/NCE). 30 male rats of wistar strain were divided into 6 groups with 5 rats each group. Group 1 rats were taken as negative control having free access to distilled water and rat feed. Group 2 rats were taken as positive control treated with mutagen cyclophosphamide (CP) at dose of 60 mg/kg b wt. for 2 days. Group 3 and 4 were treated with CT extract at dose of 100 and 200 mg/kg b wt. for 20 days. Group 5 and 6 were treated with 100 and 200 mg/kg b wt of CT extract for first 18 days and for last 2 days with CP at dose concentration of 60 mg/kg. It was found that rats treated with CT extract alone did not produce any significant changes in MnPCE, PCE/NCE ratio, CA and MI when compared with control treated rats (group 1). However in group 5 and 6 rats treated with CT extract in combination with CP a protective effect was observed against the cyclophosphamide induced cellular mutagenicity. In concluding remark Cousinia thomsonii was found to show antigenotoxic potential and also produce protective antimutagenic effects against CP induced chromosomal damage.

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Observations on Traditional Phytotherapy among the Inhabitants of Lahaul Valley through Amchi System of Medicine—A Cold Desert Area of Himachal Pradesh in North Western Himalayas, India

Cited by 26 publications

References 7 publications

Anti-diabetic, diabetic neuropathy protective action and mechanism of action involving oxidative pathway of chlorogenic acid isolated from Selinum vaginatum roots in rats

Anti-diabetic, diabetic neuropathy protective action and mechanism of action involving oxidative pathway of chlorogenic acid isolated from Selinum vaginatum roots in rats

Antitumor evaluation of two selected Pakistani plant extracts on human bone and breast cancer cell lines

Evaluation of Mutagenic and Antimutagenic Activity of Methanol Extract of Cousinia thomsonii against Cyclophosphamide

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