Observations on the pathogenesis of peritoneal adhesions: a light and electron microscopical study

Milligan, DW; Raftery, Andrew T.

doi:10.1002/bjs.1800610406

Cited by 165 publications

(84 citation statements)

References 18 publications

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“…Our study found that collagen was most prominent on Day 7, which is consistent with findings of other studies. Collagen synthesis, which reached its highest level between the fifth and seventh postoperative days (Haney and Doty, 1994;van Hinsbergh et al, 1990), allowed for adhesion maturation (Hickey, 2001;Milligan and Raftery, 1974). After 1 week, the fibrosis, which was vital to adhesion formation at this time, was less obvious in the NOCC group.…”

Section: Discussionmentioning

confidence: 99%

Use of N,O-carboxymethyl chitosan to prevent postsurgical adhesions in a rabbit double uterine horn model: a randomized controlled design

Ren

Zhao

Zhu

2016

Sci. China Life Sci.

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In previous studies, N,O-carboxymethyl chitosan has been shown to decrease the incidence and intensity of abdominal adhesions. In the present study, adhesions were induced in 220 rabbits using a double uterine horn model. Rabbits were randomized to receive an operation only or an operation+medical chitosan intraperitoneally. Twenty-two rabbits from each group were euthanized at one of five different times (Day 3, 7, 14, 28, or 42), and adhesion formation was given gross and histopathological scores. Reductions were observed in adhesion extent (P=0.0337) and tenacity (P=0.0271) as well as inflammation (P<0.0001) on Day 3 when medical chitosan was applied. Prior to Day 14, fibrosis was less obvious in the medical chitosan group (P<0.0005). The tenacity scores were significantly lower in the medical chitosan group following Day 14 (P<0.05), while the type scores were lower in the medical chitosan group following Day 28 (P<0.03). Thus, medical chitosan decreased both the gross and the histopathological scores of the induced adhesions.

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Section: Discussionmentioning

confidence: 99%

Use of N,O-carboxymethyl chitosan to prevent postsurgical adhesions in a rabbit double uterine horn model: a randomized controlled design

Ren

Zhao

Zhu

2016

Sci. China Life Sci.

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“…5-10. günlerde kollajen birikimi olur ve fibroblastlar adezyonlar içinde organize olmaya başlarlar. Yaralanmadan 1-2 ay sonra ise kollajen fibriller organize olurlar ve böylece adezyon, fibroz band yapısına olgunlaşır [13,22,23]. Böylece, adezyon oluşum mekanizması tamamlanır (Şekil 5).…”

Section: Cerrahi̇ Adezyon Oluşumunun Sebepleri̇unclassified

Cerrahi Adezyonun Önlenmesi için Yeni Bir Yaklaşım: Nanolifli Cerrahi Adezyon Bariyerleri

Şafak¹,

Karaca²,

Özalp³

2017

Tekstil ve Mühendis

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“…Mast cells have been implicated in the aethiopathology of post-operative peritoneal adhesions as well as eosinophils, macrophages, and neutrophils by influencing some of the fibroblasts and myofibroblasts biochemical-functional properties. In adhesion formation process it is possible to identify a surgical trauma in conjunction with ischemia with infection that results in an acute fibroproliferative inflammatory response [4]. Post-operative adhesion may represent the final results of an exaggerated repair mechanism.…”

Section: Introductionmentioning

confidence: 99%

Mast cells in peritoneal fluid in rats with experimentally induced peritoneal adhesions.

Hermanowicz

Dębek²,

Oksiuta³

et al. 2010

Folia Histochem Cytobiol.

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Mast cells (MC) produce, store and release many biologically active substances, especially inflammatory factors, chemotactic substances for neutrophiles, cytokines and prostaglandins. They play very important role in fibrinosis and they are an important factor in peritoneal adhesions formation and lysis. In this study we tried to evaluate role of mast cells in peritoneal adhesions formation. We estimated number of mast cells in peritoneal fluid in rats with experimentally developed peritoneal adhesions. The number of mast cells per ml was counted in flow cytometry in specimens of peritoneal fluid taken from operated rats. The fluid was taken in standarized conditions the same for each group at the first operation and during reoperation. Peritoneal cavity was washed with 0.9% Saline solution. MC were visualized using indirect immunohistochemical method LSAB with mouse antibody. The animals were divided into 4 groups. 1 st group was control group (n=20) on which the abdomen was opened and closed without any manipulations, and the reoperation was done after 72 hours. The other groups (2, 3, 4; n=20 for each group) were operated and scarification of the partial peritoneum and serosa was performed. The rats were brought back to conscious and then were reoperated respectively after 24, 72 and 168 hours after first surgery. After the laparotomy and damage of the peritoneum we observed formation of the peritoneal adhesions between intestine loops and between intestines and damaged parietal peritoneum. Also the higher number of mast cells was observed in the groups of animals with damaged peritoneum. The highest number of peritoneal adhesions was observed in the group of animals reoperated after 72 hours. After 72 and 168 hours the higher number of MC and neutrophils was observed. The difference was statistically significant. The percentage of mast cells was increasing during the experiment duration. It was different from other cells populations which decreased after 168 hours. The MC and neutrophils were cell population which changed significantly after manipulations in peritoneal cavity. It is very probable that they play an important role in peritoneal adhesions formation.

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Observations on the pathogenesis of peritoneal adhesions: a light and electron microscopical study

Cited by 165 publications

References 18 publications

Use of N,O-carboxymethyl chitosan to prevent postsurgical adhesions in a rabbit double uterine horn model: a randomized controlled design

Use of N,O-carboxymethyl chitosan to prevent postsurgical adhesions in a rabbit double uterine horn model: a randomized controlled design

Cerrahi Adezyonun Önlenmesi için Yeni Bir Yaklaşım: Nanolifli Cerrahi Adezyon Bariyerleri

Mast cells in peritoneal fluid in rats with experimentally induced peritoneal adhesions.

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