Observations on Stylar Transmitting Tissue Cells and cDNA Cloning of S-RNase in a Self-fruitful Apple 'Hirodai Ichigo'.

Determination and confirmation ofS-RNase genotypes of apple pollinators and cultivars

“…We confirmed the S 2 S 5 genotype of 'Greensleeves', which had been determined by stylar protein analysis (Bošković and Tobutt, 1999). The S-RNase genotype of 'Hirodai Ichigou' had been identified as S a S e or S 2 S 7 (Li et al, 2002). However, as S e corresponds to S 28 , not S 7 (Matsumoto et al, 2003b), its genotype should be changed to S 2 S 28 .…”

S-RNases from Self-incompatible and -compatible Apple Cultivars: Purification, Cloning, Enzymic Properties, and Pollen Tube Growth Inhibitory Activity

“…This sequence, however, is not an artifact due to any experimental error in sequencing because the Sa-RNase of Hirodai-Ichigo, which is a self-fruiting cultivar of chance seedling origin discovered at the farm of Hirosaki University, showed the same sequence. 31) It is not clear whether this mutation is characteristic in mutants changed into self-fruiting cultivars. It may be necessary to reexamine cDNA cloning of Sa-RNase in GD.…”

“…The self-fruiting in MG was increased with self-pollination rather than non-pollination (about 60z and 8z, respectively), and the seed formation was observed in self-pollination, but not in parthenocarpy. 31) Therefore, we have thought that MG apparently has a self-compatible property.…”

“…In another study of ours, however, a self-fruiting cultivar, Hirodai-Ichigo, expressed almost the same amount of S-RNases as SD. 31) Therefore, the amount of the S-RNases in MG may be responsible for showing self-compatibility, but it may be sure that the amount of S-RNases is not the only determinant conferring the self-compatible property to MG.…”