Determination and confirmation of<i>S-</i>RNase genotypes of apple pollinators and cultivars

Matsumoto, Shogo; Eguchi, Takahiro; Bessho, Hideo; Abe, K

doi:10.1080/14620316.2007.11512236

Cited by 22 publications

(16 citation statements)

References 18 publications

(22 reference statements)

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“…A total of 14 different S‐alleles were analysed in the mandate cultivars and non‐mandate parents (Table 1). The S‐alleles found in 11 reference cultivars were identical with those previously reported (Broothaerts et al 2004; Matsumoto et al 2007) except that we did not screen for S 13 (present in ‘Gravensteiner’) or S 21 (present in ‘Ribston’). One of the mandate cultivars (‘Katja’) and three of the non‐mandate parents (‘Ingrid Marie’, ‘Cortland’ and ‘Worcester Pearmain’ had also been investigated before (Broothaerts et al 2004; Matsumoto et al 2007) and found to have the same S‐allele configurations as in the present study.…”

Section: Resultssupporting

confidence: 76%

“…Possibly, there are many, albeit rather rare, S‐alleles in apple that have not yet been detected. Broothaerts et al (2004) report allelic configurations that include 3% unidentified alleles and Matsumoto et al (2007) have 4% unidentified alleles. In our material, number of unidentified alleles is much higher, 19%.…”

Section: Discussionmentioning

confidence: 99%

“…If both S‐alleles are shared, the cultivars are usually incompatible and do not yield at all except for occasional fruits obtained by bypassing the self‐incompatibility system through pollination between genetically incompatible genotypes or through selfing. In apple, allele‐specific primer pairs have been developed and used in a number of cultivar screenings (Janssens et al 1995; van Nerum et al 2001; Broothaerts 2003; Broothaerts et al 2004; Melounova et al 2005; Matsumoto et al 2007). Information about S‐locus allele composition can be used for identification of apple cultivars and determination of parentage (Kitahara et al 2005) if complemented with other marker systems.…”

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confidence: 99%

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Molecular characterisation of indigenous Swedish apple cultivars based on SSR and S-allele analysis

Garkava-Gustavsson

Brantestam²,

Sehic

et al. 2008

Hereditas

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Trees of 68 apple cultivars, aimed for preservation by the 'National Program for diversity of cultivated plants' as mandate cultivars, were analysed using a set of 10 SSR (simple sequence repeat) primer pairs and the self-incompatibility (S-)locus to evaluate genetic diversity and reveal inter-cultivar relationships. The 12 polymorphic SSR loci exhibited 2 to 15 alleles, with expected heterozygozity (H(e)) ranging from 0.36 to 0.88 and a mean of 0.74. Numerous alleles were classified as rare or unique (35% and 18% respectively). For the S-locus, a total of 14 alleles were identified in this study. Five alleles, S1-S3, S5 and S7 had frequencies ranging from 11 to 18%, whereas the remaining 9 alleles were below 6%. All sexually obtained cultivars could be distinguished with the set of SSR loci. Sports were identical with their progenitors in two cases, but differed in one SSR allele in a third case. An SSR-based dendrogram, based on Roger's genetic distances, did not reveal any clear pattern of clustering. The genetic distances were, however, correlated with a corresponding matrix obtained in a previously conducted RAPD-based study of the same cultivars. Non-mandate parents of Swedish mandate cultivars together with some other reference cultivars were included in this study to check the accuracy of allele scoring, verify parentage and compare the results of this study with those presented in previously published studies. Some discrepancies in allele sizing were revealed and the possibilities of avoiding this problem are discussed.

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Section: Resultssupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular characterisation of indigenous Swedish apple cultivars based on SSR and S-allele analysis

Garkava-Gustavsson

Brantestam²,

Sehic

et al. 2008

Hereditas

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“…S 26 was first isolated from the crab apple 'Baskatong' and has been shown to occur exclusively in crab apples (Broothaerts 2003;Matsumoto et al 2007;Verdoodt et al 1998). This is the first study reporting the presence of S 26 in M.×domestica cultivars.…”

Section: Breeding and Crop Evolutionary Perspectivesmentioning

confidence: 91%

“…Until now, several genotyping methods based on the use of S-allele specific primers were published (Broothaerts 2003;Broothaerts et al 2004;Janssens et al 1995;Kitahara et al 2000;Matsumoto et al 2007). However, specific primer-based methods could sometimes generate false-negative/positive results (Ershadi and Talaii 2007;Van Nerum et al 2001).…”

Section: Communicated By E Dirlewangermentioning

confidence: 99%

S-genotyping of old apple cultivars from the Carpathian basin: methodological, breeding and evolutionary aspects

Halász

Hegedűs

György

et al. 2011

Tree Genetics & Genomes

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Apple exhibits self-incompatibility controlled by the multiallelic S-locus. Twenty-three old apple cultivars were S-genotyped using three different approaches (allele-specific polymerase chain reaction (PCR) + cleaved amplified polymorphic sequences (CAPS), consensus PCR + sequencing and consensus PCR+CAPS) to compare the robustness and reliability of these techniques and characterise genotypes from the Carpathian basin that might be useful in resistance breeding. Best results were obtained using the ASPF3 and ASPR3S consensus primer pair that detected 96% of all alleles carried by the 23 cultivars tested. Flow cytometry analysis was also needed to control the completeness of the genotypes as was seen in case of a tetraploid cultivar with only three assigned S-alleles. The genetic disparity between the old Carpathian basin and modern apple cultivars was indicated by differences in allele frequency data (S 9 , S 24 and S 26 ) as well as single nucleotide polymorphisms in S 1 , S 2 , S 7 S 24 and S 26 and indels in S 20 and S 26 alleles. An alignment of partial genomic sequences indicated trans-specific and transgeneric evolution of S-ribonuclease alleles in the Maloideae subfamily (S 26 and S 28 ) and a possibly recent introgression event (S 1 ) between Malus×domestica and Malus sylvestris. These data suggest that the genome of old cultivars from the Carpathian basin was enriched by several Malus taxa and are free from the consequences of modern breeding. These cultivars may contribute to the widening of the genetic basis of cultivated apple and prevent genetic erosion in future commercial cultivars.

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Apple

Brown¹

2011

Fruit Breeding

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Determination and confirmation ofS-RNase genotypes of apple pollinators and cultivars

Cited by 22 publications

References 18 publications

Molecular characterisation of indigenous Swedish apple cultivars based on SSR and S-allele analysis

Molecular characterisation of indigenous Swedish apple cultivars based on SSR and S-allele analysis

S-genotyping of old apple cultivars from the Carpathian basin: methodological, breeding and evolutionary aspects

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