Observations on Paroxysmal Hemoglobinuria 1

Mackenzie, George M.; Frühbauer, Emily

doi:10.1172/jci100219

Cited by 12 publications

(5 citation statements)

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“…Agglutination following the cold phase was slight and the cells were easily resuspended, in contrast to the marked agglutinating properties of hemolytic amounts of isoantibodies or to cold agglutinins. It is further noted that hemolysin activity was present in a fraction which contained little or none of the Kline antibody, corroborating observations made many times previously (8,27) that the D-L antibody is distinct from the antibody responsible for the serologic tests for syphilis.…”

Section: H the Kinetics Of The Hemolytic Reactionsupporting

confidence: 86%

“…DISCUSSION There have been many conflicting reports concerning the properties of the D-L antibody and the characteristics of the hemolytic reaction. A number of early investigators (7,8) in their original observations demonstrated that hemolysis occurred even though fresh serum was not present in the initial cold phase. Hoover and Stone, Moss, and Cooke (4-6) demonstrated that fresh serum as a source of complement was required in the cold phase with the antibodies they were studying.…”

Section: H the Kinetics Of The Hemolytic Reactionmentioning

confidence: 99%

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Studies on Immune Human Hemolysis

Hinz

Picken

Lepow

1961

The Journal of Experimental Medicine

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Serum complement is of potential importance to clinical medicine and to human biology in immune reactions and hypersensitivity states. However, most studies on the characteristics and mechanism of action of complement have been performed on systems largely of non-human origin (1, 2). The present studies were undertaken to study a specific immune human hemolytic disorder and to use this as a model system for the study of human complement in a reaction system involving human erythrocytes and an antibody of human origin. The Donath-Landsteiner (D-L) reaction (3), responsible for the clinical syndrome of paroxysmal cold hemoglobinuria, is a reaction which requires for hemolysis that erythrocytes be incubated with antibody and fresh serum complement first at low temperatures, usually near 0°C., and then at higher temperatures, usually 37°C. It was chosen as the model for these studies because: (a) it involves human ceils and antibody and avoids the use of reagents from multiple species (rabbit antibody, sheep red cells, guinea pig complement); (b) the D-L antibody is an active hemolysin but a weak agglutinin in contrast to the isoantibodies and cold agglutinins; and (c) the biphasic nature of the reaction permits the separation of the early and late phases of complement action.The studies in the present paper were undertaken to define the kinetics of the Donath-Landsteiner reaction and served as the basis for the observations on the mechanism of action of complement in the succeeding paper. The data indicate that many conflicting earlier observations regarding the role of complement in the reaction (3--8) may be explained on the basis of variation in (a) potency and amount of antibody used, (b) sensitivity of cells, (c) amount

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Section: H the Kinetics Of The Hemolytic Reactionsupporting

confidence: 86%

Section: H the Kinetics Of The Hemolytic Reactionmentioning

confidence: 99%

Studies on Immune Human Hemolysis

Hinz

Picken

Lepow

1961

The Journal of Experimental Medicine

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“…As a result of infection with the spirochete of syphilis (2,3), the patient produces a factor that, under proper conditions, hemolyzes his own erythrocytes. Hemoglobinuria is thus a dramatic manifestation of the ability of an infectious agent to induce the body to destroy one of its tissues.…”

mentioning

confidence: 99%

“…Hemoglobinuria is thus a dramatic manifestation of the ability of an infectious agent to induce the body to destroy one of its tissues. Such "auto-immunization" is not dependent on any specific blood group antigen (2,3), and the serum antibody is active not only against the patient's erythrocytes but also against erythrocytes from other individuals.…”

mentioning

confidence: 99%