Observations on basophil degranulation as an indicator of antigen-antibody reaction

A Study on the Detection of Human Reagins with Rat Peritoneal Mast Cells

“…Rabbit basophils were reported to undergo degranu lation in the presence of allergic sera and antigen [12][13][14][15][16][17]. The reliability of this reaction to distinguish allergic from non-allergic sera has recently been questioned [18][19][20] due to high degranulating activity of control sera (in the absence of antigen) and lack of re producibility. In addition, there was no apparent relationship between the basophil degranulating and reaginic activities of Scphadex G-200 or DEAE-Sephadex fractions of allergic human sera [21].…”

The Serological Diagnosis of Penicillin Allergy

“…The technique was later modified (84,85). The test must be interpreted as an in vitro equivalent of basopenia in certain allergic reactions (76), but it has been regardetl witb great scepticism (12,27). It is tecbnically clilhcult, antl it is not known whether it bears a tiirect etirrelation to the occurrence of skin-sensiti/.ing or agglutinating antibodies.…”

Role of Human Reaginic and Hemagglutinating Antibodies in the Indirect Rabbit Basophil Degranulation Reaction

“…However, the immunoglobulin nature of the human, drug-specific antibody mediating rabbit basophil degranulation, and its correspondence with the rcagin class, have not yet been elucidated. Furthermore, the relatively few applications [7][8][9] of the rabbit basophil dcgranulation test which have been made for in vitro detection of the various pollinoses, for example, ragweed pollen allergy, have given equivocal or contradictory results. Indeed, K ravis et al [9] have questioned whether the antibody mediating basophil degranulation is related in any way to the reaginic-type anti body.…”