A Study on the Detection of Human Reagins with Rat Peritoneal Mast Cells

A Modified Rat Mast Cell Technique for the Detection of Human IgE-Type Reagins

“…For this purpose, glutaraldehyde was used as the fixative agent. The present study shows that the results employing the fixation procedure were similar to those using the previously described technique [3]. This indicated that the fixation procedure had little, if any, effect on mast cell changes that occurred in the presence of human scrum and antigen or human serum or antigen alone.…”

“…It lias been previously shown [3] that normal rat serum is effective in reducing non-specific mast cell changes provoked merely by contact with human sera. In the initial phases of this study it was found that similar non-specific effects took place in the absence of the rat sera during the fixation of the rat mast cells.…”

“…As lias been previously described [1][2][3][4][5], rat peritoneal mast cells undergo specific morphological changes in the presence of human allergic sera and specific antigen. The results of these studies indicated that IgE-type reagins were involved in the cytopathic changes.…”

“…As part of these studies [3], it was observed that once the mast cells were removed from the peritoneal cavity of the rat they could undergo spontaneous morphological changes approximately 2 h after removal. Thus, the number of allergic sera that could be assayed with appro priate controls using the rat mast cell technique was restricted by this time limit.…”

The Effect of Laundry Detergent Containing <i>B. subtilis</i> Enzyme in Producing Sensitization Reactions in Guinea Pigs

“…The purpose of the present study, was to simulate conditions of consumer use of enzymecontaining detergents in the guinea pigs in order to establish whether ex posure of these animals to an alcalase-containing detergent by inhala tion, topical and abraded skin application and oral ingestion routes would yield allergic manifestations. Examination of these animals on long-term exposure revealed sensitization of the jejunum and the pres ence of circulating antibodies to alcalase as determined by the rat mast cell technique [12][13][14].…”