Observations of New Phenomena in the Fluorescence Spectrum of a Diphosphopyridine Nucleotide-Linked Dehydrogenase

Winer, Alfred D.; Novoa, William B.; Schwert, George W.

doi:10.1021/ja01581a067

Cited by 48 publications

(9 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If these proteins would be saturated sequentially, an increase of fluorescence enhancement would result. Secondly, further increases of fiuorescence yield have been observed in the formation of ternary complexes by NAD-dependent dehydrogenases [52,53]. A third possibility is the contribution of non-cytosolic systems.…”

Section: Properties Of Cytosolic Nadh-binding Bitesmentioning

confidence: 99%

State of Oxidation-Reduction and State of Binding in the Cytosolic NADH-System as Disclosed by Equilibration with Extracellular Lactate/Pyruvate in Hemoglobin-Free Perfused Rat Liver

Bücher¹,

Brauser²,

Conze³

et al. 1972

Eur J Biochem

163

View full text Add to dashboard Cite

where subscripts e and c refer to extracellular and cytosolic spaces, respectively, several lines of evidence support the assumption that near-equilibrium conditions exist for processes 1 through 3 during the normoxic steady state of rat liver [l-31.Since 1965, in studies with the isolated hemoglobin free perfused organ [4,5], extensive use was made of this system in the direction from right to left, using the ratio interest was focussed on the other direction in order to obtain detailed information on the NADH system of liver oytosol which was previously not accessible. Extracellular [lactate]/[pyruvate] was used as experimental variable, and dependent changes in cytosolic NADH were observed by a variety of methods, in particular by continuous measurement of fluorescence and absorbance of perfused liver a t pyridine-nucleotide-specific wavelengths.By means of mathematical extrapolation of titration results in a double reciprocal plot derived on the basis of the mass action law, the maximal

show abstract

Section: Properties Of Cytosolic Nadh-binding Bitesmentioning

confidence: 99%

State of Oxidation-Reduction and State of Binding in the Cytosolic NADH-System as Disclosed by Equilibration with Extracellular Lactate/Pyruvate in Hemoglobin-Free Perfused Rat Liver

Bücher¹,

Brauser²,

Conze³

et al. 1972

Eur J Biochem

163

View full text Add to dashboard Cite

show abstract

“…A lactic dehydrogenase-DPNH com plex could be demonstrated by its characteristic fluorescence spectrum (80). Winer & Schwert (79) described quan titatively the influence of pH on the kinetics of lactate dehydrogenation, as suming a lactate-DPN-and a lactate-DPNH-protein complex respectively in which an SH group is involved.…”

Section: Oligo-and Polysaccharidesmentioning

confidence: 99%

Carbohydrate Metabolism

Holzer¹

1959

Annu. Rev. Biochem.

View full text Add to dashboard Cite

The following abbreviations are used: A CTH for adrenocorticotropic hormone; ADP for adenosine diphosphate; AMP for adenosine diphosphate; ATP for adenosine triphosphate; DPN for oxidized diphosphopyridine nucleotide; DPNH for reduced diphosphopyridine nucleotide; TPN for oxidized triphosphopyridine nucleotide; TPNH for reduced triphosphopyridine nucleotide; UDP for uridine diphosphate; UMP for uridine monophosphate; UTP for uridine triphosphate. • The author is grateful to Dr. H.-J. Boltze for the translation. 171Annu. Rev. Biochem. 1959.28:171-222. Downloaded from www.annualreviews.org Access provided by University of California -San Francisco UCSF on 02/04/15. For personal use only. Quick links to online content Further ANNUAL REVIEWSIn pancreatic j uice of the rat, the concentration of a-amylase is often so high that the enzyme crystallizes spontaneously (14).Cellulose metabolism has been investigated in a variety of molds and bacteria [(15 to 23); for a summary see (24)]. According to Hash & King (19,20), various �-glucosidases are present in the fungus Myrothecium verrucaria, the specificity of which is dependent on the chain length of the substrate. Cellobiose was not found to be an obligatory precursor of glucose in cellulose breakdown. "Cellulose dextrins" (hexasaccharides, etc.) may be formed from cellobiose by transglucosidation. High-speed-centrifuged par ticles from Acetobacter xylinum were shown by Glaser (21) to contain an enzyme which produces cellulose from UDP-glucose. Similar to glycogen synthesis from UDP-gl�cose a primer is required, such as cellodextrins.In various grasses, structure, synthesis, and breakdown of polyfructosans have been investigated in their dependence upon various, particularly sea sonal conditions by Schlubach (25). Avigad & Feingold (26) investigated the fructosides produced ' from sucrose by a corynebacterium. For the synthesis of hyaluronic acid in streptococci, it has been reported by Markovitz et al. (27) that glucose is a precursor of the glucuronic acid and glucosamine moieties. Tritiated UDP-glucuronic acid together with UDP-N-acetyl glucosamine, ATP, and N-acetyl glucosamine-l-phosphate were incubated with strain A streptococci and found to produce appreciable Annu. Rev. Biochem. 1959.28:171-222. Downloaded from www.annualreviews.org Access provided by University of California -San Francisco UCSF on 02/04/15. For personal use only. CARBOHYDRATE METABOLISM 173 amounts of hyaluronic acid. This indicates that the synthesis of hyaluronic acid is very likely to occur via the UDP compounds. The occurrence in group A streptococci of the UDP compounds mentioned has been ascertained chromatographically (28).Glaser & Brown (29) reported that the formation of chitin in a cell-free preparation from Neurospora crassa utilizes UDP-N-acetyl glucosamine, and requires an acetyl glucosamine polymer as a primer:UDP-acetyl glucosamine + (acetyl glucosamine),,� UDP + (acetyl g!ucosamine)n-t1 (2) The decomposition of chitin has been investigated by Berger & Reynolds (30) with a chiti...

show abstract

“…It is, of course, not necessary to have the "reporter" compound covalently attached to the enzyme. Changes in the fluorescence of coenzymes reflecting the conformative response to other ligands have been observed and studied (118,698,865). In such systems the coenzyme may be said to serve as a fluorescent probe of the conformation of the active site.…”

Section: '-Umpmentioning

confidence: 99%

Conformational Adaptability in Enzymes

Citri¹

1973

Advances in Enzymology - And Related Areas of Molecular Biology

View full text Add to dashboard Cite

Observations of New Phenomena in the Fluorescence Spectrum of a Diphosphopyridine Nucleotide-Linked Dehydrogenase

Cited by 48 publications

References 2 publications

State of Oxidation-Reduction and State of Binding in the Cytosolic NADH-System as Disclosed by Equilibration with Extracellular Lactate/Pyruvate in Hemoglobin-Free Perfused Rat Liver

State of Oxidation-Reduction and State of Binding in the Cytosolic NADH-System as Disclosed by Equilibration with Extracellular Lactate/Pyruvate in Hemoglobin-Free Perfused Rat Liver

Carbohydrate Metabolism

Conformational Adaptability in Enzymes

Contact Info

Product

Resources

About