Observation on the capping of spleen lymphocytes from scrapie infected mice after treatment with anti Ig

Golden Syrain hamsters were inoculated intracerebrally with the hamster-adapted 263K strain of scrapie virus, and the evolution of in vitro cell fusing activity induced by brain suspensions was compared with brain infectivity titers and histological changes. Cell-fusing activity abruptly appeared 4 weeks after inoculation, 1 week before the earliest detectable histopathological changes, at an infectivity level of 7.6 log 50% lethal doses per g of brain. Cell-fusing activity was sustained throughout the remaining 4 weeks of the incubation period and the subsequent 1- to 3-week stage of clinical illness but did not increase with the logarithmic progression of infectivity, which reached a level of 11 log 50% lethal doses per g in the agonal stage of disease. Gliosis was most sensitively detected by a monoclonal antibody reacting with astrocyte intermediate filaments in an indirect immunofluorescence test, anticipating histological recognition of gliosis and spongiform change by 1 to 2 weeks. In vitro cell-fusing activity is thus one of the earliest known biological markers (apart from infectivity itself) of experimental scrapie infection.

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Observation on the capping of spleen lymphocytes from scrapie infected mice after treatment with anti Ig

Cited by 3 publications

References 4 publications

Further studies on changes in immunoglobulin G in the sera and CSF of Herdwick sheep with natural and experimental scrapie

Further studies on changes in immunoglobulin G in the sera and CSF of Herdwick sheep with natural and experimental scrapie

Pathogenesis of mouse scrapie: Distribution of agent in the pulp and stroma of infected spleens

Experimental scrapie in golden Syrian hamsters: temporal comparison of in vitro cell-fusing activity with brain infectivity and histopathological changes

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