Observation of circulating tumour cells in patients with non-small cell lung cancer by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction in peroperative period

Ge, Ming; De, Shi; Wu, Qing; Wang, Mei; Li, Liang Bin

doi:10.1007/s00432-005-0059-3

Cited by 38 publications

(41 citation statements)

References 17 publications

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“…An increase in CTC during and/or after surgery has also been observed in studies that assessed peripheral blood samples using different detection methods (18)(19)(20)(21). Interestingly, CK19 and CEA transcripts have shown a different peri-operative kinetic when used as markers of CTC dissemination (20). The value of CK19 mRNA during the operation was significantly higher than that of pre-operation and postoperation; in contrast, the post-operative levels of CEA mRNA were significantly higher than those of preoperation and those of operative day.…”

Section: Detection and Prognostic Value Of Ctc In Early Nsclcmentioning

confidence: 61%

“…In this respect, Hashimoto and collaborators (15) found that the median number of CTC in PV samples increased from 4 to 60 in 30 consecutive primary lung cancer patients who underwent lobectomy through open thoracotomy. An increase in CTC during and/or after surgery has also been observed in studies that assessed peripheral blood samples using different detection methods (18)(19)(20)(21). Interestingly, CK19 and CEA transcripts have shown a different peri-operative kinetic when used as markers of CTC dissemination (20).…”

Section: Detection and Prognostic Value Of Ctc In Early Nsclcmentioning

confidence: 72%

“…Hashimoto and collaborators (15) reported that the increase in CTC count after surgery was not significantly associated with the sequence of vessel interruption (pulmonary artery first versus PV first). In contrast, Ge and co-workers (20) found higher levels of CEA mRNA in patients had the ligation of the pulmonary artery first as compared with PV first. However, it must be emphasized that no difference in survival using different sequences of pulmonary vessel ligation has been described (18).…”

Section: Detection and Prognostic Value Of Ctc In Early Nsclcmentioning

confidence: 79%

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Clinical utility of circulating tumor cells in patients with non-small-cell lung cancer

Gallo

Luca

Maiello

et al. 2017

Transl. Lung Cancer Res.

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Several different studies have addressed the role of the circulating tumor cells (CTC) in nonsmall-cell lung cancer (NSCLC). In particular, the potential of CTC analysis in the early diagnosis of NSCLC and in the prediction of the outcome of patients with early and advanced NSCLC have been explored. A major limit of these studies is that they used different techniques for CTC isolation and enumeration, they employed different thresholds to discriminate between high-and low-risk patients, and they enrolled heterogeneous and often small cohort of patients. Nevertheless, the results of many studies are concordant in indicating a correlation between high CTC count and poor prognosis in both early and advanced NSCLC. The reduction of CTC number following treatment might also represent an important indicator of sensitivity to therapy in patients with metastatic disease. Preliminary data also suggest the potential for CTC analysis in the early diagnosis of NSCLC in high-risk individuals. However, these findings need to be confirmed in large prospective trials in order to be transferred to the clinical practice.The molecular profiling of single CTC in NSCLC might provide important information on tumor biology and on the mechanisms involved in tumor dissemination and in acquired resistance to targeted therapies. In this respect, xenografts derived from CTC might represent a valuable tool to investigate these phenomena and to develop novel therapeutic strategies.

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Section: Detection and Prognostic Value Of Ctc In Early Nsclcmentioning

confidence: 61%

Section: Detection and Prognostic Value Of Ctc In Early Nsclcmentioning

confidence: 72%

Section: Detection and Prognostic Value Of Ctc In Early Nsclcmentioning

confidence: 79%

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Clinical utility of circulating tumor cells in patients with non-small-cell lung cancer

Gallo

Luca

Maiello

et al. 2017

Transl. Lung Cancer Res.

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“…Several studies [16][17][18][19][20][21][22][23][24][25] , published in this area with a focus on lung cancer, have provided knowledge of the clinical significance of tumor markers for the detection of MRD. Of these the most specific marker appears to be Lunx protein 16,17,25 , although some other markers appear to be applicable for MRD detection in lung cancer, especially CEA (ref.…”

Section: Discussionmentioning

confidence: 99%

“…Cells expressing CK19 were already circulating during the operation, and cells that expressed CEA were frequently disseminated only in the postoperative period. Surgical manipulation may lead to the dissemination of tumor cells into the blood circulation but this could be prevented by ligation of the pulmonary vein before pulmonary artery ligation 24 . In 2002, Masato et al focused on small-cell lung cancer (SCLC), which tends to hematogeníc rapid progression.…”

Section: Detection Of Minimal Residual Disease In Lung Cancermentioning

confidence: 99%

Detection of minimal residual disease in lung cancer

Chudáček¹,

Bohanes²,

Klein³

et al. 2014

BIOMED PAP

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Effect of Vein-First vs Artery-First Surgical Technique on Circulating Tumor Cells and Survival in Patients With Non–Small Cell Lung Cancer

et al. 2019

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IMPORTANCEIt is important to develop a surgical technique to reduce dissemination of tumor cells into the blood during surgery.OBJECTIVE To compare the outcomes of different sequences of vessel ligation during surgery on the dissemination of tumor cells and survival in patients with non-small cell lung cancer. DESIGN, SETTING, AND PARTICIPANTSThis multicenter, randomized clinical trial was conducted from December 2016 to March 2018 with patients with non-small cell lung cancer who received thoracoscopic lobectomy in West China Hospital, Daping Hospital, and Sichuan Cancer Hospital. To further compare survival outcomes of the 2 procedures, we reviewed the Western China Lung Cancer database (2005-2017) using the same inclusion criteria.INTERVENTIONS Vein-first procedure vs artery-first procedure. MAIN OUTCOMES AND MEASURESChanges in folate receptor-positive circulating tumor cells (FR + CTCs) after surgery and 5-year overall, disease-free, and lung cancer-specific survival.RESULTS A total of 86 individuals were randomized; 22 patients (25.6%) were younger and 64 (74.4%) older than 60 years. Of these, 78 patients were analyzed. After surgery, an incremental change in FR + CTCs was observed in 26 of 40 patients (65.0%) in the artery-first group and 12 of 38 (31.6%) in the vein-first group (P = .003) (median change, 0.73 [interquartile range (IQR), −0.86 to 1.58] FU per 3 mL vs −0.50 [IQR, −2.53 to 0.79] FU per 3 mL; P = .006). Multivariate analysis confirmed that the artery-first procedure was a risk factor for FR + CTC increase during surgery (hazard ratio [HR], 4.03 [95% CI,]; P = .005). The propensity-matched analysis included 420 patients (210 with vein-first procedures and 210 with artery-first procedures). The vein-first group had significantly better outcomes than the artery-first group for 5-year overall survival (73.6% [95% CI, 64.4%-82.8%] vs 57.6% [95% CI, 48.4%-66.8%]; P = .002), disease-free survival (63.6% [95% CI, 55.4%-73.8%] vs 48.4% [95% CI, 40.0%-56.8%]; P = .001), and lung cancer-specific survival (76.4% [95% CI, 67.6%-85.2%] vs 59.9% [95% CI, 50.5%-69.3%]; P = .002). Multivariate analyses revealed that the artery-first procedure was a prognostic factor of poorer 5-year overall survival (HR, 1.65 [95% CI, 1.07-2.56]; P = .03), disease-free survival (HR, 1.43 [95% CI, 1.01-2.04]; P = .05) and lung cancer-specific survival (HR = 1.65 [95% CI, 1.04-2.61]; P = .03). CONCLUSIONS AND RELEVANCELigating effluent veins first during surgery may reduce tumor cell dissemination and improve survival outcomes in patients with non-small cell lung cancer.TRIAL REGISTRATION ClinicalTrials.gov identifier: NCT03436329

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Observation of circulating tumour cells in patients with non-small cell lung cancer by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction in peroperative period

Cited by 38 publications

References 17 publications

Clinical utility of circulating tumor cells in patients with non-small-cell lung cancer

Clinical utility of circulating tumor cells in patients with non-small-cell lung cancer

Detection of minimal residual disease in lung cancer

Effect of Vein-First vs Artery-First Surgical Technique on Circulating Tumor Cells and Survival in Patients With Non–Small Cell Lung Cancer

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