Oat Bran, Rye Bran, and Soybean Hull Increase Goblet Cell Volume Density in the Small Intestine of the Golden Hamster a Histochemical and Stereologic Light-Microscopic Study

Lundin, E.; Zhang, Jie‐Xian; Huang, Chaoran; Reuterving, Carl-Olof; Hallmans, Göran; Nygren, Charlotte; Stenling, Roger

doi:10.3109/00365529309096039

Cited by 23 publications

(18 citation statements)

References 33 publications

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“…The precise mechanisms by which xylanase affects mucin synthesis are not clear, but our results provide evidence of an association between lower intestinal viscosity and enhanced mucin output. Recent studies have shown xylanase activity in wheat bran [20], and our findings are consistent with previous observations that consumption of dietary bran stimulates mucin production in the small intestine [16] and the colon [2], thus enhancing protection of the mucosa. When the effects of a wheat-based diet on goblet cell mucins were compared with the effects of the maizebased diet, there were no noticeable differences in acidic mucins either in the small intestine, caecum or large intestine.…”

Section: Discussionsupporting

confidence: 81%

The influence of diet on the mucin carbohydrates in the chick intestinal tract

Sharma¹,

Fernandez

Hinton

et al. 1997

CMLS, Cell. mol. life sci.

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The presence of nutrients in the intestinal lumen is a major factor influencing bacterial colonization in poultry. This study was conducted to investigate the effects of poultry feed on viscosity of intestinal contents and on mucin carbohydrates by comparing jejunal supernatants and the histochemical composition of goblet cells in chicks reared to 5 weeks of age on either a conventional maize-based diet or a wheat-based diet or a wheat diet supplemented with 0.1% xylanase. Regional differences in the distribution of the neutral, carboxylated and sulphated mucins were demonstrated using conventional histochemical techniques, while a panel of lectins was used to study alterations in glycoconjugate synthesis of mucins in chicks fed different diets. 1) Feeding a diet supplemented with xylanase lowered the viscosity but increased the amount of neutral, carboxylated and sulphated mucins in the jejunum. 2) In chicks fed a maize-based diet, neutral mucins increased in the surface and upper crypt goblet cells of the small and large intestines but decreased in the caecum. 3) Feeding a diet supplemented with xylanase modified crypt-surface glycosylation of N-acetylglucosamine residues and resulted in loss of sialic acid residues in the small and large intestines. These results indicate that the constituents of poultry feed, in particular the consumption of a diet supplemented with xylanase, lead to changes in intestinal viscosity and mucin composition which are associated with alterations in the goblet cell glycoconjugates of the chick intestinal tract.

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Section: Discussionsupporting

confidence: 81%

The influence of diet on the mucin carbohydrates in the chick intestinal tract

Sharma¹,

Fernandez

Hinton

et al. 1997

CMLS, Cell. mol. life sci.

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“…Results from this study confirm previous observations that consumption of dietary xylanase by decreasing intestinal viscosity stimulates mucin production in the small intestine, caecum and large intestine [21]. Our observations are also consistent with the finding that consumption of dietary bran stimulates mucin production in the small intestine and the colon, so enhancing protection of the mucosa [19,35]. Although lectin histochemical studies have been used to investigate the distribution of glycoconjugates on the intestinal epithelium [16,23,24], no data on the effects of diet-microbial interaction on the carbohydrate residues of the chick intestinal goblet cells have been reported.…”

Section: Research Articlesupporting

confidence: 82%

Diet influences the colonisation of Campylobacter jejuni and distribution of mucin carbohydrates in the chick intestinal tract

Fernandez¹,

Sharma

Hinton³

et al. 2000

CMLS, Cell. Mol. Life Sci.

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The objectives of this study were to evaluate the effect of diet on the colonisation by Campylobacter jejuni of the chick caeca, and to determine whether the viscosity of the intestinal contents and mucin carbohydrates were altered by the diet. The diets investigated were maize based, wheat-based or wheat-based supplemented with xylanase. The xylanase-supplemented diet reduced the viscosity and lowered the numbers of Camp. jejuni. Feeding the enzyme-supplemented diet increased the amount of neutral and sulphated mucins in the goblet cells of the small and large intestines and caecum. An abundance of sulphated and carboxylated mucins was seen in the surface goblet cells of the large intestine with the maize- and wheat-based diets. Both the diet supplemented with xylanase and the maize diets increased crypt-surface glycosylation of the sialic acid residues. The analysed data from the combined sites showed significant differences in the amount of neutral and acidic mucins when comparing the wheat and the wheat plus xylanase diets. However, no changes were shown in the staining intensity of sulphated mucins between the three diets. Significant differences in the glycosylation of sialic acid and in the N-acetylglucosamine residues were shown between dietary groups. These results provide evidence that the wheat diet supplemented with xylanase leads to greater changes in the mucin composition and carbohydrate expression of goblet cell glycoconjugates, which are associated with a reduction in intestinal viscosity and decreased numbers of Camp. jejuni.

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“…Changes in goblet cell density (Lundin et al, 1993) and mucin synthesis (Satchithanandam, Klurfeld, Calvert, & Cassidy, 1996) were reported in earlier studies as a result of dietary fibre supplementation to diets. In the present study, diets supplemented with various NDCs had no significant diet, day and diet · day interaction effect on either crypt depth (P = 0.852) or goblet cells per crypt (P = 0.792) in rats ( Fig.…”

Section: Colon Crypt Depth and Goblet Cellsmentioning

confidence: 91%

Evaluation of gastrointestinal transit in rats fed dietary fibres differing in their susceptibility to large intestine fermentation

Paturi

Butts

Monro

et al. 2012

Journal of Functional Foods

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Non-digestible carbohydratesShort-chain fatty acids Titanium dioxide Transit time A B S T R A C TThe rate and extent of in situ digesta transit after ingestion of diets containing dietary fibres differing in their susceptibility to large intestine fermentation were investigated. One hundred and twenty rats were fed diets containing 7.5% cellulose, inulin, potato fibre or maize starch for 3 days, then the same diets with titanium dioxide (TiO 2 ) for 3 days, followed by diets without TiO 2 for 2 days. In all diets, TiO 2 ratios rapidly increased within 24 h and reached a maximum level in duodenum, caecum and colon within 2-3 days. Inulin, potato fibre and maize starch-fed rats showed higher levels of caecal short-chain fatty acids, lower faecal polysaccharide concentrations, and reduced faecal output than the rats fed cellulose. Inulin was highly susceptible to caecal microbial fermentation compared to the other dietary fibres. Transit of these dietary fibres through the GI tract was rapid, and the rate of digesta transit was not affected by dietary fibre fermentability in the large intestine.

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Oat Bran, Rye Bran, and Soybean Hull Increase Goblet Cell Volume Density in the Small Intestine of the Golden Hamster a Histochemical and Stereologic Light-Microscopic Study

Cited by 23 publications

References 33 publications

The influence of diet on the mucin carbohydrates in the chick intestinal tract

The influence of diet on the mucin carbohydrates in the chick intestinal tract

Diet influences the colonisation of Campylobacter jejuni and distribution of mucin carbohydrates in the chick intestinal tract

Evaluation of gastrointestinal transit in rats fed dietary fibres differing in their susceptibility to large intestine fermentation

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