2010
DOI: 10.1007/s13277-010-0114-9
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O-glycosylation of MUC1 mucin in prostate cancer and the effects of its expression on tumor growth in a prostate cancer xenograft model

Abstract: MUC1 mucin is up-regulated and aberrantly glycosylated in many human epithelial carcinomas. Over-expression of MUC1 has also been implicated in prostate cancer, but neither the role of MUC1 in the cancer progression nor the mucin O-glycosylation has been fully elucidated. In this study, we characterized the O-glycans on MUC1 when over-expressed in the human prostate cancer cell line C4-2B(4). We found that the main O-glycan consisted of the neutral core 2 oligosaccharide Galβ3(Galβ3/4GlcNAcβ6)GalNAc-ol, with m… Show more

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Cited by 18 publications
(15 citation statements)
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References 32 publications
(40 reference statements)
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“…PC-3 and DU145 cells are expected to have only core 1-based O-glycans and appear to be restricted in their ability to form branched chains since the activity of C2GnT1 was undetectable in spite of mRNA expression. In contrast, LNCaP cells are capable of synthesizing a multitude of O-glycan structures with core 1 and 2 structures [51]. …”
Section: Discussionmentioning
confidence: 99%
“…PC-3 and DU145 cells are expected to have only core 1-based O-glycans and appear to be restricted in their ability to form branched chains since the activity of C2GnT1 was undetectable in spite of mRNA expression. In contrast, LNCaP cells are capable of synthesizing a multitude of O-glycan structures with core 1 and 2 structures [51]. …”
Section: Discussionmentioning
confidence: 99%
“…The mucin family encompasses a diverse set of high molecular weight glycoproteins characterized by the presence of O-linked oligosaccharides to serine or threonine residues [3, 4]. MUC1 protein expression has been found to be significantly elevated in several cancers including CaP [4, 5] and is usually accompanied by altered glycosylation [6, 7]. In addition, MUC1 expression in cancer is usually characterized by a diffuse cytoplasmic staining pattern compared to apically restricted expression typically found in normal tissues [8–11].…”
Section: Introductionmentioning
confidence: 99%
“…In addition, MUC1 expression in cancer is usually characterized by a diffuse cytoplasmic staining pattern compared to apically restricted expression typically found in normal tissues [8–11]. MUC1 over-expression has been reported to allow malignant cells to evade host immunological defenses and to promote metastasis through a loss of cell–cell and cell–extracellular matrix contact [7, 1216]. …”
Section: Introductionmentioning
confidence: 99%
“…[13][14][15][16][17] Eight core structures have been described for mucin-type oligosaccharides (Table S- sialic acids (Neu) and/or sulfate groups. 1,5,18 The combination of different core structures, chain elongation, branching, linkages and the variety of peripheral and terminal residues results in a high degree of mucin oligosaccharide heterogeneity, 1 with dozens to hundreds of diverse oligosaccharides densely substituting the mucin protein backbone 19 in a 'bottle-brush' configuration 20 ( Figure 1A).…”
Section: Introductionmentioning
confidence: 99%