O-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme

Acet, Ömür; Baran, Talat; Erdönmez, Demet; Aksoy, Neşe Hayat; Alacabey, İhsan; Menteş, Ayfer; Odabaşı, Mehmet

doi:10.1016/j.chroma.2018.03.022

Cited by 53 publications

(17 citation statements)

References 40 publications

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“…In the last decade, there has been an increasing trend in the enrichment of biomarkers with IMAC in various biotechnological and biomedical applications before mass spectrometric analysis for their detection in biological samples [7, 9, 42–44, 47–50, 14,15,51–60]. IMAC has become an important technique in the purification of histidine‐tagged proteins and phosphoproteins in recent years.…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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“…[100] Fourier transform infrared spectroscopy (FT-IR) shows the differences between carboxymethyl chitosan and HPFC (Figure 5d). The stretching vibration of OÀ H shows strong peaks at 3556 cm À 1 ; the peaks at 3478 cm À 1 , 3416 cm À 1 and 3233 cm À 1 are due to NÀ H for primary and secondary amines; [101] and the peak at 1617 cm À 1 also illustrates the presence of NÀ H. The peak at 1640 cm À 1 is attributed to the C=N bond, [102,103] which indicates the successful grafting of polyethylenimine onto carboxymethyl chitosan by glutaraldehyde during the Schiff base reaction.…”

Section: Hyperbranched Polyethylenimine-functionalized Carboxymethyl mentioning

confidence: 99%

Removal of Mercury Ions from Aqueous Solutions by Crosslinked Chitosan‐based Adsorbents: A Mini Review

Zhang

Crini

Lichtfouse

et al. 2020

The Chemical Record

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Abatement of mercury emissions in air and waters has become a global challenge due to the toxicity of mercury species for life, yet actual remediation techniques are limited. In particular, adsorption of mercury ions onto solids is widely used but most adsorption techniques are not specific, and in turn, removal efficiency is lower. Adsorbents developed so far include activated carbon, clay, bentonite, cellulose and chitosan. Chitosan derivatives have recently attracted research attention for water purification because their molecular frames contain a large amount of À NH 2 and À OH groups that can chelate with metal ions specifically. This manuscript reviews recent advances in chitosan-based adsorbents designed to remove mercury ions from wastewater. Focus is placed on their design, synthesis, characterization, adsorption properties, adsorption mechanisms and applications.

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“…Cryogels are formed by the polymerization of gelation agents at minus temperatures [21,22]. They ensure some facilities such as little residence time because of short diffusion route, low-pressure drop etc.…”

Section: Introductionmentioning

confidence: 99%

“…Particle embedded cryogels have been taken attention of scientists because of the combination of significant properties of cryogels and embedded particles. Cryogels offer perfect mass flow with supermacroporous structures whereas pumice particles present high adsorption capacity with high surface area and ligand density [22]. The immobilization of enzymes has been conducted onto some solid surfaces in the literature such as carbon nanotube [25], activated carbon [26], nanozeolite [27], silica material [21] and pumice particle [23].…”

Section: Introductionmentioning

confidence: 99%

α-Amylase Immobilized Composite Cryogels: Some Studies on Kinetic and Adsorption Factors

Acet

Inanan

Acet

et al. 2021

Appl Biochem Biotechnol

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Stability of enzymes is significant factor for their industrial feasibility. α-amylase is an important enzyme for some industries i.e., textile, food, paper and pharmaceutics.. Pumice particles (PPa) are non-toxic, natural, low-cost alternative adsorbents with high adsorption capacity. In this study, Cu 2+ ions were attached onto pumice particles (Cu 2+ -APPa). Then, Cu 2+ -APPa embedded composite cryogel was synthesized (Cu 2+ -APPaC) via polymerization of gelforming agents at minus temperatures. Characterization studies of the Cu 2+ -APPaC cryogel column was performed by X-ray fluorescence spectrometer (XRF), scanning electron microscopy (SEM), and Brunauer, Emmett, Teller (BET) apparatus. The experiments were carried out in a continuous column system. α-amylase was adsorbed onto Cu 2+ -APPaC cryogel with maximum amount of 858.7 mg/g particles at pH 4.0. Effects of pH and temperature on the activity profiles of the free and the immobilized α-amylase were investigated, and results indicate that immobilization did not alter the optimum pH and temperature values. kcat value of the immobilized α-amylase is higher than that of the free α-amylase while KM value increases by immobilization. Storage and operational stabilities of the free and the immobilized αamylase were determined for 35 days and for 20 runs, respectively.

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O-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme

Cited by 53 publications

References 40 publications

Recent trends in sorbents for bioaffinity chromatography

Recent trends in sorbents for bioaffinity chromatography

Removal of Mercury Ions from Aqueous Solutions by Crosslinked Chitosan‐based Adsorbents: A Mini Review

α-Amylase Immobilized Composite Cryogels: Some Studies on Kinetic and Adsorption Factors

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