Inoculation of cowpea with biofertilizers (rhizobia + mycorrhiza), and spraying them with the mycoinsecticide Metarhizium anisoplae has been shown to improve growth and grain yield. However, the treatment may impair the technological properties of the grains, which have not yet been investigated. This study is a followup to our recent study on the effect of selected biofertilizers and mycoinsecticide in three agroecological zones of Cameroon, aimed at evaluating the physical properties and the protein content of cowpea seeds as affected by these bio-inoculants. Spraying cowpea plants at flowering with the mycoinsecticide M. anisopliae and/or inoculating cowpea seeds at sowing with biofertilizers (rhizobia, mycorrhizae) had no significant effect on the protein content, water absorption capacity, and the percentage of seed coat. They instead significantly affected the physical properties of seeds, such as the seed weight, length, geometric diameter, and sphericity, but this varied from one agroecological zone to another. Whereas the seed weight was increased in agroecological zone V, a significant decrease was observed in zones I and II. The changes in weight reflected those of diameter and volume as confirmed by the correlation analysis. Application of biofertilizers had no significant effect on the water absorption capacity, protein, and coating percentage of cowpea seeds, suggesting that the main qualities of cowpea seeds for industrial utilization remained the same. The increased yield generally associated with these treatments may result from changes in the geometric size, which depends on the agroecological zone.