NUTRITIONAL STUDIES ON LACTOBACILLUS HETEROHIOCHI

Indigenous Poultry Feed Microflora Response to Ethyl Alcohol and Buffered Propionic Acid Addition

“…Concentrations of ethyl alcohol in liquids above 15% generally cause inactivation of most organisms in a vegetative state of metabolism, although spores from spore forming bacteria are much more resistant (Dagley et al 1950;Hugo 1967;Harold 1970;Ingram and Buttke 1984;Caldwell and Murray 1986). However, several species of the genus Lactobacillus and the bacterium Zymomonas mobilis which produces ethyl alcohol can grow in much higher concentrations of ethyl alcohol (Demain et al 1961;Uchida 1975a,b;Carey and Ingram 1983). It is also possible that the various organic materials in the feed such as proteins and other biomolecules are somewhat protective as reduced antiseptic activities of alcohols have been reported when they were used in the presence of organic material (Gershenfeld et al 1951).…”

Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei

“…Effects of cysteine, ascorbic acid and milk on the growth of L. casei CCDM 198 and biogenic amines degradation: To support bacteria growth and/or biogenic amines degradation, 1% (w/v) cysteine (concentration recommended by supplier Laktoflora) or 0.1% (w/v) ascorbic acid (Demain et al, 1961) were added to a 50 mL MRS/2 medium (composition described in growth conditions) before inoculation.…”

“…Effects of media on growth pattern of selected LAB strains: Many studies have demonstrated the positive effect of oxygen scavenging agents (cysteine and ascorbic acid) on viability of probiotic bacteria (Dave and Shah, 1997;Demain et al, 1961;Rickes et al, 1949;Shah, 2000). Oxygen reduces the growth of these bacteria, and the use of cysteine and ascorbic acid may lower redox potential by scavenging oxygen, thus affecting their growth (Shah, 2000).…”