Nutritional studies on fruit trees

Mulder, D.

doi:10.1007/bf01373640

Cited by 9 publications

References 4 publications

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Variation in mineral composition of Cox's Orange Pippin apples

Wilkinson¹,

Perring²

1961

J Sci Food Agric

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and Ultrasorb S.C. 120!240. (To decolorise the extracts, the weight of charcoal needed is about ten times that of the plant material extracted.)The following solutions are then percolated through the column at the rate of about I ml. pcr rnin. : (i) The plant extract containing Fe-EDTA, washed through with IOO ml. of water ; (ii) IOO ml. of 5% ethanol ; (iii) 250 ml. of 25:& ethanol (this elutes the Fe-EDTh in high yield but not always quantitatively). [The column eluates arc colourless exccpt a t stage (iii) where traces of plant colouring matter also appear.! The eluate from stage (iii) is evaporated down and spatted on to the paper chromatograms. Pa$er chroinatographyAfter development with the phenol-water system as described above, iron chelates show as dark spots on the dried chromatogram in ultra-violet light. The paper is then stained b y the following procedure : (i) dip in h'iS0, solution, 0.01';; in methanol ; (ii) remove most of the solvent in a cool air stream and then expose the papcr to NH, vapour ; (iii) dip in dimethylglyoxime solution, 0.1:; in ethanol. The chelating agents then S~O M . as white spots on a pink background. Acknowledgment rock, Grays, Esses. The activated carbon used \vas the gift of the Rritiah Carbo-Sorit LTnion Ltd., \Vest Thur-The Rcscarch Station Long Asliton Rristol Rvccived IS January, t$w ; aiiirndd manuscript 2 5 May, IOGO

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