thiamine, pyridoxine, nicotinic acid, folic acid, ascorbic acid. Without thiamine practically no growth of the fungus took place ; omission of pyridoxine had nearly as adverse an effect. Raffinose and cellobiose were the best carbon sources ; glucose also gave a good yield of mycelium. Statistical analysis of factorial experiments indicated that a correct balance between the mineral constituents of the medium and between the carbon and nitrogen sources is essential in order to obtain a high yield of mycelium.Apple scab is one of the commonest orchard diseases in temperate regions. The disease attacks all parts of the tree and when infection of the fruit is severe the yield of marketable apples may be decreased by as much as 50 yo.This disease is caused by the fungus Venturia inaequalis (Cooke) Winter, an . ascomycete. The conidial phase, which is the more important stage from a pathological standpoint, was a t one time called Fusicladium dendriticum (Wallr.) Fol.; this stage is found on all living parts of the host. The perithecial stage is less noticeable and occurs only on over-wintered fallen leaves. Most of the work done on Venturia inaequalis in the past has concentrated on the genetics of disease resistance and susceptibility. Johnstone (1931) suggested that resistance to the fungus was not due to any single factor and appeared to depend on the physiological relationship between host and parasite, while the work of Keitt & Langford (1941) indicated that it might be connected with the nutrition of the fungus. The nutritional requirements of V. inaequalis have not previously been comprehensively investigated; the present paper describes results of work along these lines.
lMETHODSStock cultures of Venturia inaequalis were grown on malt-extract agar slopes containing apple leaf decoction; this MEAL medium was prepared as follows. Air-dried 'Bramley's Seedling' apple leaves (25 g.) were boiled in 500 ml. distilled water for 30 min. Malt extract ( 5 g.) and agar (25 g,) were dissolved in distilled water and the filtered leaf decoction added to this mixture, which was then made up to a final volume of 1 1. The inoculum for all experiments was prepared by adding 15 ml. sterile distilled water to a 6 x 2 in. tube 26 G. Microb. XII